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The map label for this gene is proA [H]

Identifier: 148827052

GI number: 148827052

Start: 314679

End: 315932

Strand: Direct

Name: proA [H]

Synonym: CGSHiGG_01815

Alternate gene names: 148827052

Gene position: 314679-315932 (Clockwise)

Preceding gene: 148827051

Following gene: 148827055

Centisome position: 16.67

GC content: 41.23

Gene sequence:

>1254_bases
ATGTTAGAACAAATGGGTAAACAAGCCAAAGATGCTGCATTTATCTTGGCTCAACTCACCACTGCTGAAAAAAATTGTGC
ATTATCCATTATTGCAGAACAACTCGAACAACAAGCACCGCTTATCTTAGCCGAAAACGCAAAAGATATTGAACTTGCCA
AACAAAATGGATTATCGGATGCCTTGATTGATCGCCTACTGCTCACACAAGAACGTTTACAAGGCATTGCTAATGATGTA
CGCCACGTTATTTCTCTAGCAGATCCCGTGGGGAAAATCATAGACGGCGGTACATTGGATAGCGGACTTAAAATCGAGCG
CGTACGCACTCCGCTCGGCGTCATTGGCACAATTTATGAAGCTCGCCCAAATGTGACCATTGATGTGGCAAGTCTTTGCC
TTAAAACTGGCAATGCGGTGATTTTACGCGGCGGTAAAGAAACACAATTTTCTAACAAGATTTTAATCGAAGTTGTGCAA
AATGCTTTAGAGCAAGCAGGCTTACCAAAATTTGCGGTGCAAGCCATTACCGATCCAAACCGTGAACTCGTTATGCAATT
ATTAAAACTAGATCGCTATGTGGATATGATTATTCCTCGTGGTGGTGCGGGTTTACATGAATTGTGTAAACAACATTCTA
CTATTCCTGTTATTGTGGGTGGCGTGGGTGTTTGCCATATTTTCGTTGAAAAAAGTGCGGATCAAAATAAAGCGGTTTTT
GTTATTGATAACGCCAAAACCCAACGTCCAAGCACTTGTAACACATTGGAAACATTGTTAGTTCAACATTCTATTGCTGA
AGAATTTTTACCTAAACTTGTCTCTCACCTTTCCGCTAAAAACGTAAAATATCATGCAAAATCCACCGCACTTAATATAT
TGAAACAAGCGGGTGCGAATGTCTGCGAAGTGACAGAAAAAGAATTGCGAAAAGAATGGGGATCATTGGATTTGAATGTG
GTTGTTGTGGAAGATATTCATGCCGCTATTGAGCATATTCGCCAATATGGTACGCAACATTCTGAAAGTATTTTAACGTC
CTCACAAAACCTAGCCCGTCAATTTATCAATCAAGTTGATGCGGCAGCCGTTTATGTGAATGCAAGCACACGCTTTACTG
ATGGCGGACAATTTGGATTAGGCGCAGAAGTTGCAGTGAGCACCCAAAAACTTCATGCTCGTGGCCCAATGGGATTAGAA
GCATTAACCAGTTATAAATGGGTTTGTGAAGGCGAATATACTGTCCGCAAATAA

Upstream 100 bases:

>100_bases
TCGAATGGGGGTTGCCGGTGTTTAATTACCAAGAACTCACATCGGAAGAACAAGATAAGCTGCCAAATAAAAAGAAATAA
ATACAATAAAGGGAATAACT

Downstream 100 bases:

>100_bases
AACCTAAAGTGCGGTAATTTTTAGAAGAGATTATTTGCATATAAAAAAGTCCGCGAAATGCGGACTTTTTCATTATCTTT
ATTTGTCAGATTTACCCAAA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLEQQAPLILAENAKDIELAKQNGLSDALIDRLLLTQERLQGIANDV
RHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYEARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQ
NALEQAGLPKFAVQAITDPNRELVMQLLKLDRYVDMIIPRGGAGLHELCKQHSTIPVIVGGVGVCHIFVEKSADQNKAVF
VIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLSAKNVKYHAKSTALNILKQAGANVCEVTEKELRKEWGSLDLNV
VVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVDAAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTSYKWVCEGEYTVRK

Sequences:

>Translated_417_residues
MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLEQQAPLILAENAKDIELAKQNGLSDALIDRLLLTQERLQGIANDV
RHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYEARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQ
NALEQAGLPKFAVQAITDPNRELVMQLLKLDRYVDMIIPRGGAGLHELCKQHSTIPVIVGGVGVCHIFVEKSADQNKAVF
VIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLSAKNVKYHAKSTALNILKQAGANVCEVTEKELRKEWGSLDLNV
VVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVDAAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTSYKWVCEGEYTVRK
>Mature_417_residues
MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLEQQAPLILAENAKDIELAKQNGLSDALIDRLLLTQERLQGIANDV
RHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYEARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQ
NALEQAGLPKFAVQAITDPNRELVMQLLKLDRYVDMIIPRGGAGLHELCKQHSTIPVIVGGVGVCHIFVEKSADQNKAVF
VIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLSAKNVKYHAKSTALNILKQAGANVCEVTEKELRKEWGSLDLNV
VVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVDAAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLE
ALTSYKWVCEGEYTVRK

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=414, Percent_Identity=37.1980676328502, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI62912457, Length=414, Percent_Identity=37.1980676328502, Blast_Score=268, Evalue=7e-72,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=62.5, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=36.6265060240964, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=36.8674698795181, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324899, Length=426, Percent_Identity=35.6807511737089, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI21357643, Length=414, Percent_Identity=35.5072463768116, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45406; Mature: 45406

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLEQQAPLILAENAKDIELAKQNGLSD
CCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCHH
ALIDRLLLTQERLQGIANDVRHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCHHHHHHHHH
ARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQNALEQAGLPKFAVQAITDPN
CCCCCEEHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
RELVMQLLKLDRYVDMIIPRGGAGLHELCKQHSTIPVIVGGVGVCHIFVEKSADQNKAVF
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCCEEE
VIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLSAKNVKYHAKSTALNILKQAGAN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCC
VCEVTEKELRKEWGSLDLNVVVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVD
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
AAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTSYKWVCEGEYTVRK
CEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCEEECCCEEECC
>Mature Secondary Structure
MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLEQQAPLILAENAKDIELAKQNGLSD
CCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHCCCHH
ALIDRLLLTQERLQGIANDVRHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCHHHHHHHHH
ARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQNALEQAGLPKFAVQAITDPN
CCCCCEEHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
RELVMQLLKLDRYVDMIIPRGGAGLHELCKQHSTIPVIVGGVGVCHIFVEKSADQNKAVF
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCCEEE
VIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLSAKNVKYHAKSTALNILKQAGAN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCC
VCEVTEKELRKEWGSLDLNVVVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVD
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
AAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTSYKWVCEGEYTVRK
CEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA