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The map label for this gene is metF [H]

Identifier: 148826947

GI number: 148826947

Start: 189822

End: 190700

Strand: Direct

Name: metF [H]

Synonym: CGSHiGG_01105

Alternate gene names: 148826947

Gene position: 189822-190700 (Clockwise)

Preceding gene: 148826942

Following gene: 148826951

Centisome position: 10.06

GC content: 36.29

Gene sequence:

>879_bases
ATGAGCTACGCGAAAGAAATTGATACATTAAATCAACATATTGCAGATTTTAATAAAAAAATTAATGTCTCCTTTGAATT
TTTTCCACCTAAAAACGAAAAAATGGAAACCCTTCTATGGGATTCAATTCATCGTTTAAAAGTATTAAAGCCTAAATTTG
TGTCAGTCACTTACGGTGCAAATTCGGGAGAACGTGACCGCACTCACGGCATTGTGAAAGCCATTAAACAAGAAACTGGC
TTAGAAGCCGCACCACATTTAACTGGAATTGATGCCACACCTGAAGAATTAAAACAAATTGCGAGAGATTATTGGGATAG
TGGTATTCGCCGTATTGTTGCGTTACGCGGTGACGAACCTAAAGGTTACGCAAAAAAACCATTTTATGCGTCAGATCTTG
TGGAATTACTCCGTTCTGTCGCTGATTTTGATATTTCTGTAGCCGCTTATCCTGAAGTTCATCCAGAAGCAAAATCCGCA
CAAGCAGACTTAATTAATTTAAAACGTAAAATTGATGCAGGTGCAAACCACGTCATTACACAATTTTTCTTTGATATTGA
AAACTACCTACGTTTTCGTGATCGTTGTGCATCAATTGGTATTGATACTGAAATCGTACCCGGTATTTTACCTGTTACTA
ATTTTAAACAACTCCAAAAAATGGCATCATTCACTAATGTGAAAATTCCAGCGTGGTTAGTTAAAGCCTATGATGGTTTG
GATTATGATCCAACTACACGTAATCTTGTAGCAGCAAGTGTTGCAATGGATATGGTAAAAATTTTATCTCGCGAAGGCGT
GAATGACTTCCACTTTTATACATTAAATCGTAGTGAATTAACTTATGCTATCTGTCATATGTTAGGTGTAAGACCTTAA

Upstream 100 bases:

>100_bases
ATTAATAATAATCATATTTTTAATGAATAAATTGAAATTGACTCATATTAAAACCTATTTTATTGTACAAATACTATTTT
AGAATCCTAAAGGAAGCCTT

Downstream 100 bases:

>100_bases
TTATCACAATAATATATTTCTTTTCAAACACAAAATAAGGGCGATATTTCGCCCTTATACAAAACTAAAAAAAATTCAAC
CGCACTTTATTATTTTATGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DYDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Sequences:

>Translated_292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DYDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
>Mature_291_residues
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETGL
EAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQ
ADLINLKRKIDAGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGLD
YDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=33.5689045936396, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1790377, Length=290, Percent_Identity=73.1034482758621, Blast_Score=456, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=29.8561151079137, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=29.8561151079137, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=32.312925170068, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=301, Percent_Identity=29.2358803986711, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33069; Mature: 32938

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DYDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDAGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DYDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]