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The map label for this gene is folE [H]

Identifier: 148826951

GI number: 148826951

Start: 192742

End: 193956

Strand: Direct

Name: folE [H]

Synonym: CGSHiGG_01125

Alternate gene names: 148826951

Gene position: 192742-193956 (Clockwise)

Preceding gene: 148826947

Following gene: 148826952

Centisome position: 10.21

GC content: 40.33

Gene sequence:

>1215_bases
ATGCTTACATTGGATCAAGCTCGCTCGAAAATGCTCGAACAGATTCCCTTCCCGACTCAAACTGAATACCTAAATTTAAA
AGAAGCCGCCAATCGAATTTGTGCAGAAGATATTATTTCGCCTATCAATGTTCCCTCTTTCGATAACTCAGCAATGGATG
GTTATGCTGTACGTTTATCGGATTTACAACAATCTTTAACCCTTTCTGTCGCAGGGAAATCTTTTGCAGGTAACCCATTC
CAAGGGGAATGGCCATCAAAAAGTGCGGTCAGAATTATGACTGGTGCGATGATTCCTGAAGGCGCAGATGCAGTGATAAT
GCAAGAGCAAGTAACACTCAATGAAGACGGCACCGTCACTTTTAGTGAATTACCTAAACCAAATCAAAACATCCGTCGTA
TTGGTGAAGATGTAAAGAAAGGCGATGTGGTATTAGAACAAGGCGCACCGCTCACCCCAGTTTCCTTGCCATTATTAGCC
TCTCTAGGCATTGCAGAAGTAAAATGCTATCGTCAATTAAAAGTCGGCGTACTCTCAACTGGCGATGAACTTGTGGAAGT
CGGCAAACCATTACAAAGCGGACAAATCTATGACACTAACCGTTTCACCGTAAAATTATTACTCGAAAAACTACACTGTG
AAGTCATTGATTTAGGACTATTACCTGATAACGAAGCTGAATTTGAAAAAGCATTTATTGCCGCACAAAGCCAAGCCGAT
TTAGTGATTACCAGTGGTGGTGTTTCCGTTGGTGAAGCGGATTTCACTAAAGCAGTATTGGAAAAAGTAGGCAAAGTAAA
TTTCTGGAAAATTGCAATCAAACCCGGCAAACCATTCGCTTTTGGTAAATTAGAAAATGCTTGGTTCTGTGGTTTACCCG
GCAATCCTGTTTCCGCATTAATAACATTTTATCAACTTGTTCAGCCATTAATCGCTAAACTTCAAGGCCAAAAACAATGG
AAAAAACCACCGCACTTTTCAGCCATTGCTACAATGAATTTGAAAAAAGCAGTGGGACGTTTAGACTTCCAACGGGGTTT
TTATTACATCAATGAACAAGGTCAAATGGAAGTACAATCAGTTGGTTTTCAAGGCTCTCATCTCTTTAGTGCTTTTGTAA
AAAGCAATTGTTTTATCGTGTTAGAACAAGAACGTGGCAATGTCTCTGCGGGCGAAACAGTGACAATTGAGCCTTTCAAT
CATTTATTAGGATAA

Upstream 100 bases:

>100_bases
AAAGGATAATAGGATGGTAAAATGAGAAAAATTGCAAATTTGAATGAATTAGGCTTTAGATATAGAATAGCTGACAAATT
TTATCAAATAAGGATTTTCT

Downstream 100 bases:

>100_bases
AAAATGATCGAACTTAGTCACGAAGAAGAATTGCGTTATAACCGTCAAATTATTCTTAAAAGCGTAGATTTTGACGGGCA
AGAAAAACTTAAAGCAAGCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLTLDQARSKMLEQIPFPTQTEYLNLKEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
QGEWPSKSAVRIMTGAMIPEGADAVIMQEQVTLNEDGTVTFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLHCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALITFYQLVQPLIAKLQGQKQW
KKPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQMEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG

Sequences:

>Translated_404_residues
MLTLDQARSKMLEQIPFPTQTEYLNLKEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
QGEWPSKSAVRIMTGAMIPEGADAVIMQEQVTLNEDGTVTFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLHCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALITFYQLVQPLIAKLQGQKQW
KKPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQMEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG
>Mature_404_residues
MLTLDQARSKMLEQIPFPTQTEYLNLKEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
QGEWPSKSAVRIMTGAMIPEGADAVIMQEQVTLNEDGTVTFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLHCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALITFYQLVQPLIAKLQGQKQW
KKPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQMEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=396, Percent_Identity=34.0909090909091, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI10880983, Length=396, Percent_Identity=34.0909090909091, Blast_Score=188, Evalue=8e-48,
Organism=Escherichia coli, GI1787049, Length=404, Percent_Identity=56.9306930693069, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25147659, Length=334, Percent_Identity=32.9341317365269, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17562606, Length=327, Percent_Identity=29.9694189602446, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136978, Length=375, Percent_Identity=29.6, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24638841, Length=375, Percent_Identity=29.6, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 44195; Mature: 44195

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLDQARSKMLEQIPFPTQTEYLNLKEAANRICAEDIISPINVPSFDNSAMDGYAVRLS
CCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHH
DLQQSLTLSVAGKSFAGNPFQGEWPSKSAVRIMTGAMIPEGADAVIMQEQVTLNEDGTVT
HHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEE
FSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLASLGIAEVKCYRQLKVGVLST
HHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEECC
GDELVEVGKPLQSGQIYDTNRFTVKLLLEKLHCEVIDLGLLPDNEAEFEKAFIAAQSQAD
CHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCC
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSAL
EEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCEEECCCCCHHHHH
ITFYQLVQPLIAKLQGQKQWKKPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQMEVQS
HHHHHHHHHHHHHHCCCHHCCCCCCCHHEEHHHHHHHHHHHHHHCCEEEECCCCCEEHHH
VGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFNHLLG
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCC
>Mature Secondary Structure
MLTLDQARSKMLEQIPFPTQTEYLNLKEAANRICAEDIISPINVPSFDNSAMDGYAVRLS
CCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHH
DLQQSLTLSVAGKSFAGNPFQGEWPSKSAVRIMTGAMIPEGADAVIMQEQVTLNEDGTVT
HHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEE
FSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLASLGIAEVKCYRQLKVGVLST
HHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEECC
GDELVEVGKPLQSGQIYDTNRFTVKLLLEKLHCEVIDLGLLPDNEAEFEKAFIAAQSQAD
CHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCC
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSAL
EEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCEEECCCCCHHHHH
ITFYQLVQPLIAKLQGQKQWKKPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQMEVQS
HHHHHHHHHHHHHHCCCHHCCCCCCCHHEEHHHHHHHHHHHHHHCCEEEECCCCCEEHHH
VGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFNHLLG
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]