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The map label for this gene is azoR [H]

Identifier: 148826836

GI number: 148826836

Start: 93210

End: 93794

Strand: Reverse

Name: azoR [H]

Synonym: CGSHiGG_00490

Alternate gene names: 148826836

Gene position: 93794-93210 (Counterclockwise)

Preceding gene: 148826838

Following gene: 148826825

Centisome position: 4.97

GC content: 34.53

Gene sequence:

>585_bases
ATGAGCAACGTATTAGTATTAAAATCTAGCATTTCAGGAAATAACTCTCAGACTAATCAATTAGTTGATTATGCTGTCGA
AAAATTACAAGGTAATAACATTATCGTACGCGATTTATCTCAACAACCGCTTCCATATTTTGATACCGTAGCTGCAATTG
CTGTACGTGGGGAACCAAAAACAATAGAAGAAAAACAACTTTTGGCGTTGTCTGATGAATTAATCGAAGAATTAAAAAAC
GCACAGACATTAATAATTGGTGCACCAATGTATAATTTAAACATTCCAACACAATTAAAAAGCTATTTTGATTTTATCGC
ACGTCCGAGCGTTACTTTCCAATATACAGCAAAGGGTCCTGAAGGTTTGTTGAAAGGCAAAAAAGTGATTGTGTTATGTG
CATTTGGTGGGTTGTATGATGAAGAAAATTTAGTAACACAATATATGAAATCAATTTTAGGTTTTATTGGCATCACAGAT
GTGCAATTTGTTTATGCACAAGGCATTGGATTCGGGCCAGAGGCGATTGAAAAAGCCCAAGCGTCCGCAAAAAATAAAAT
TAATGAAATTGTAGCAGCCCTTTAA

Upstream 100 bases:

>100_bases
TTTACAGAAAGGAAATAATGTTTTTCTGACTAGGGGCTTTATATCCTAAAAATAAAGGCTAAAATAACCGCACTTTTAAA
TACACTGTTAAGGAATAAAA

Downstream 100 bases:

>100_bases
TCCTCAGATAATCTACAAAACATAAGAAAATGTATTACAAACAAAAAAGTGCGGTAATTTACCGCACTTTTTTTCTTATT
TCTTTCGTTCAACCCATTTT

Product: DNA topoisomerase I

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSNVLVLKSSISGNNSQTNQLVDYAVEKLQGNNIIVRDLSQQPLPYFDTVAAIAVRGEPKTIEEKQLLALSDELIEELKN
AQTLIIGAPMYNLNIPTQLKSYFDFIARPSVTFQYTAKGPEGLLKGKKVIVLCAFGGLYDEENLVTQYMKSILGFIGITD
VQFVYAQGIGFGPEAIEKAQASAKNKINEIVAAL

Sequences:

>Translated_194_residues
MSNVLVLKSSISGNNSQTNQLVDYAVEKLQGNNIIVRDLSQQPLPYFDTVAAIAVRGEPKTIEEKQLLALSDELIEELKN
AQTLIIGAPMYNLNIPTQLKSYFDFIARPSVTFQYTAKGPEGLLKGKKVIVLCAFGGLYDEENLVTQYMKSILGFIGITD
VQFVYAQGIGFGPEAIEKAQASAKNKINEIVAAL
>Mature_193_residues
SNVLVLKSSISGNNSQTNQLVDYAVEKLQGNNIIVRDLSQQPLPYFDTVAAIAVRGEPKTIEEKQLLALSDELIEELKNA
QTLIIGAPMYNLNIPTQLKSYFDFIARPSVTFQYTAKGPEGLLKGKKVIVLCAFGGLYDEENLVTQYMKSILGFIGITDV
QFVYAQGIGFGPEAIEKAQASAKNKINEIVAAL

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=201, Percent_Identity=53.2338308457711, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21233; Mature: 21102

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLVLKSSISGNNSQTNQLVDYAVEKLQGNNIIVRDLSQQPLPYFDTVAAIAVRGEPK
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEECCCCC
TIEEKQLLALSDELIEELKNAQTLIIGAPMYNLNIPTQLKSYFDFIARPSVTFQYTAKGP
CCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHHCCCCEEEEEECCCC
EGLLKGKKVIVLCAFGGLYDEENLVTQYMKSILGFIGITDVQFVYAQGIGFGPEAIEKAQ
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
ASAKNKINEIVAAL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SNVLVLKSSISGNNSQTNQLVDYAVEKLQGNNIIVRDLSQQPLPYFDTVAAIAVRGEPK
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEECCCCC
TIEEKQLLALSDELIEELKNAQTLIIGAPMYNLNIPTQLKSYFDFIARPSVTFQYTAKGP
CCCHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHHCCCCEEEEEECCCC
EGLLKGKKVIVLCAFGGLYDEENLVTQYMKSILGFIGITDVQFVYAQGIGFGPEAIEKAQ
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHH
ASAKNKINEIVAAL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA