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The map label for this gene is pntA [H]

Identifier: 148826833

GI number: 148826833

Start: 86477

End: 88015

Strand: Direct

Name: pntA [H]

Synonym: CGSHiGG_00465

Alternate gene names: 148826833

Gene position: 86477-88015 (Clockwise)

Preceding gene: 148826832

Following gene: 148826834

Centisome position: 4.58

GC content: 42.76

Gene sequence:

>1539_bases
ATGTTAATTGGTGTACCTAGAGAGCTGCTTGAGAATGAGAGCCGTGTGGCGGCAACGCCGAAAACGGTTCAGCAAATATT
GAAATTGGGCTTTGACGTGATCGTGGAACACGATGCAGGTTTTAAAGCTAGTTTTGAAGATCAAGCATTTTTAGAGGCAG
GCGCGAAGATCGGTACGTCGGCTGAAATTTGGCAATCGGATATTATCTTCAAAGTAAATGCACCGACAGATGAAGAAATC
GCTCAAATGAAGGAAGGGGCAACTCTCGTGAGCTTTATTTGGCGCATGCAAAATCCTGAGTTAATGAAAAAACTCACCGC
GAAGAAAATTAATGTGTTAGCAATGGATGCGGTGCCTCGTATTTCTCGTGCGCAAGCATTGGATGCGCTTTCTTCTATGG
CGAATATTTCTGGTTATCGTGCTGTCATTGAAGCTGCGCATGAATTCGGTAGCTTCTTTACTGGGCAAATTACTGCTGCA
GGTAAAGTTCCACCAGCAAAAGTGTTGGTTATTGGTGCGGGTGTGGCTGGTTTGGCGGCAATTGGTGCAGCAAATAGCCT
TGGGGCGATTGTTCGTGCTTTTGACTCTCGTCCTGAAGTGAAAGAGCAAGTTCAGAGTATGGGCGCGAGCTTCTTAGAAA
TTGATTTCAAAGAAGAAGGTGGCAGTGGCGATGGTTATGCGAAAGTGATGTCGGAAGAATTTAACCGTCGTGCGATGGAG
CTTTATGCAGAACAAGCTAAAGAAGTCGATATTATTATTACTACGGCAGCCATTCCGGGTAAACCAGCGCCTCGTTTAAT
CACCAAAGAAATGGTGGATTCAATGAAACCAGGTTCGGTGATTGTGGATTTAGCAGCTGCAACTGGCGGTAACTGTGAAT
ATACACAAGCAGGGAAAGTTGTTACAACTGAAAACCAAGTGAAAGTGATTGGTTATACAGATTTCCCAAGCCGTTTACCG
ACGCAATCTTCTCAACTTTATGGCACAAACTTAGTCAATTTATTAAAACTTTTGTGTAAAGAGAAAGATGGCAATATCAA
TATTGATTTTGAAGATGTGGTTCTTCGTGGTGTAACTGTTGTTCGTGATGGGGAAGAAATTCCACCAGCACAAATTCAAG
TGTCTGCACAACCAAAACAAGAAACTAAAGCTGCACCAGTAGCAGAGAAAAAAGAATCTAAACCAACAGATCCTCGTGTG
AAATACGGTGTGATAGCAGGTGTGGGTGTGTTATTCCTTTGGTTAGCTTCTGTTGCGCCCGCTGCATTTTTATCACATTT
CACCGTATTTGTTTTAGCTTGTGTGGTGGGCTATTACGTGGTTTGGAATGTTAGCCATGCACTTCACACACCATTAATGG
CAGTAACAAATGCAATTTCAGGCATTATTATTGTAGGCGCGTTATTACAAATTCGTCAGCCAACAGGCAATCTATTTATC
GATGCGTTAGCTTTTGTGGCGATTTTGGTGGCAAGCATCAATATTTTTGGTGGTTTCCGTGTCACACAACGTATGTTAGC
AATGTTTAGAAAAGGTTAA

Upstream 100 bases:

>100_bases
TACATTTGCATCCGTTTGACTTTTTGTCATTTTAACGATGAATAGGCGAAGGTTTGTTCATTAATTTCATTAACCCAATC
ATCAATCAGAGGGAAACAGT

Downstream 100 bases:

>100_bases
GGAGCGCACAATGTCTGAAGGTTTAGTACAGGCTGCGTATATTTTAGCAGCATTACTTTTCATTATGAGCTTGGCAGGAC
TTTCTAAACATGAAACTGCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGATLVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVIAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG

Sequences:

>Translated_512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGATLVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVIAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
>Mature_512_residues
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTSAEIWQSDIIFKVNAPTDEEI
AQMKEGATLVSFIWRMQNPELMKKLTAKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAA
GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKVVTTENQVKVIGYTDFPSRLP
TQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTVVRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRV
KYGVIAGVGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
DALAFVAILVASINIFGGFRVTQRMLAMFRKG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=541, Percent_Identity=48.6136783733826, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI122939153, Length=541, Percent_Identity=48.6136783733826, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI1787887, Length=512, Percent_Identity=73.6328125, Blast_Score=743, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=521, Percent_Identity=48.9443378119002, Blast_Score=462, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR016040
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54985; Mature: 54985

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTS
CCCCCCHHHHCCCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
AEIWQSDIIFKVNAPTDEEIAQMKEGATLVSFIWRMQNPELMKKLTAKKINVLAMDAVPR
HHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKV
HHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCE
VTTENQVKVIGYTDFPSRLPTQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEE
VRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRVKYGVIAGVGVLFLWLASVAP
EECCCCCCCCEEEEECCCCHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIGVPRELLENESRVAATPKTVQQILKLGFDVIVEHDAGFKASFEDQAFLEAGAKIGTS
CCCCCCHHHHCCCCCEECCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
AEIWQSDIIFKVNAPTDEEIAQMKEGATLVSFIWRMQNPELMKKLTAKKINVLAMDAVPR
HHHHCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCC
ISRAQALDALSSMANISGYRAVIEAAHEFGSFFTGQITAAGKVPPAKVLVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDSRPEVKEQVQSMGASFLEIDFKEEGGSGDGYAKVMSEEFNRRAME
HCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMKPGSVIVDLAAATGGNCEYTQAGKV
HHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCE
VTTENQVKVIGYTDFPSRLPTQSSQLYGTNLVNLLKLLCKEKDGNINIDFEDVVLRGVTV
EEECCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEECCHHHHHHHCEEE
VRDGEEIPPAQIQVSAQPKQETKAAPVAEKKESKPTDPRVKYGVIAGVGVLFLWLASVAP
EECCCCCCCCEEEEECCCCHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAFLSHFTVFVLACVVGYYVVWNVSHALHTPLMAVTNAISGIIIVGALLQIRQPTGNLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
DALAFVAILVASINIFGGFRVTQRMLAMFRKG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]