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The map label for this gene is waaA [H]

Identifier: 148826713

GI number: 148826713

Start: 1764278

End: 1765558

Strand: Direct

Name: waaA [H]

Synonym: CGSHiEE_08940

Alternate gene names: 148826713

Gene position: 1764278-1765558 (Clockwise)

Preceding gene: 148826711

Following gene: 148826714

Centisome position: 97.31

GC content: 39.27

Gene sequence:

>1281_bases
GTGGCGTTTTTTTATACCAGCTTGCTTTTAATTTGCCAGCCGTTGATATTGTGTTTTATTGGCTTGCTCAGTGTTAAATC
TCCACGTTATCGTCAGCGTTTGGCTGAACGTTATGGTTTTTATGGCAATACCTCTTGCCCACCACCACAAGGGATCTTTA
TCCACGCAGCCTCCGTTGGAGAAGTGATTGCTGCGACACCGCTAGTTCGCCAATTGCAACAAGATTATCCGCATTTATCC
ATTACTTTTACAACTTTTACTCCCACAGGTTCAGAACGCGTAAAAGCCACTTTTGGAAACAGCGTTTTTCATTATTATCT
CCCCTTTGATTTGCCTTTTTCGATCCATCGTTTTATTAATTTTGTACAACCTAAATTATGTATCGTGATGGAAACAGAAC
TTTGGCCTAATCTTATTCATCAATTATTTTTACGCAATATCCCATTTGTCATCGCTAATGCTCGTTTATCCGCACGTTCT
GCACATCGTTACGGAAAAATAAAAGCGCGCTTACAAACAATGTGGTCGCAAATTAGCTTGATTGCAGCACAAGATCATAT
TAGCGGAAAACGCTATGCCATTTTGGGATATCCAAAGGAAAAATTGAATATCACGGGCAATATTAAATATGATCTCTCGA
TCACTGATGAATTACGTGAAAAGATTGATGATTTACGATCTCTTTGGGTAAAAAATCGTCCGATTTGGATTGCAGCCAGT
ACGCATAACGGAGAAGATGAAATCATTTTAAAATCTCACCGCACTTTGTTAGCAAAATACCCAAATTTATTGTTATTGCT
CGTTCCACGCCATCCAGAGCGTTTTAATGTGGTGGCTGATTTACTTAAAAAGGAAAAATTTCAATTTATTCGCCGTTCGA
CAAATGAGTTACCAAATGAAAATACTCAAGTAATTTTAGGCGATAGTATGGGCGAACTGATGTTGATGTATGGCATTTCC
GATATTGCTTTTGTGGGGGGAAGCCTTGTGAAACATGGGGGGCATAATCCTTTAGAACCTCTTGCATTTAAAATGCCAGT
GATTACTGGCAAGCATACTTTCAATTTCCCCGAGATATTTCGAATGCTGGTAGAAGTACAAGGTGTGTTGGAAGTTAATT
CAACCGCAGATGCTTTGGAACGTGCGGTAGAAGCCTTATTAAATTCAAAAGAGGCTAGCGAACGTTTAGGCAATGCAGGT
TATGAAGTGCTGATGGAAAACCGAGGGGCATTACAGCGTTTGTTAGATTTATTAAAACCTTATTTGGAGCGTAATGTATG
A

Upstream 100 bases:

>100_bases
ATTTTTTACAATCATTGCAACGCTAATTGTAGGCATATTTTCAATCCTTTTTTTGCTATACTAGCCGAAATTTTTTCAAG
TATAACCGAATTTCATTTAT

Downstream 100 bases:

>100_bases
CGAGCGTGATTTATCCTGGCACTTTTGACCCGATTACGAACGGTCATTTAGATATAATTGAAAGAAGTGCGGTGATTTTT
CCTCGTGTTTTAGTGGCGGT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNTSCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHLS
ITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARS
AHRYGKIKARLQTMWSQISLIAAQDHISGKRYAILGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAAS
THNGEDEIILKSHRTLLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGIS
DIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAG
YEVLMENRGALQRLLDLLKPYLERNV

Sequences:

>Translated_426_residues
MAFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNTSCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHLS
ITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARS
AHRYGKIKARLQTMWSQISLIAAQDHISGKRYAILGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAAS
THNGEDEIILKSHRTLLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGIS
DIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAG
YEVLMENRGALQRLLDLLKPYLERNV
>Mature_425_residues
AFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNTSCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHLSI
TFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARSA
HRYGKIKARLQTMWSQISLIAAQDHISGKRYAILGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAAST
HNGEDEIILKSHRTLLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGISD
IAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAGY
EVLMENRGALQRLLDLLKPYLERNV

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=422, Percent_Identity=50.4739336492891, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48506; Mature: 48375

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNTSCPPPQGIFIHAASVG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHH
EVIAATPLVRQLQQDYPHLSITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFIN
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHH
FVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARSAHRYGKIKARLQTMWSQISL
HCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAQDHISGKRYAILGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAAS
HEECCCCCCCEEEEEECCHHHEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC
THNGEDEIILKSHRTLLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNE
CCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NTQVILGDSMGELMLMYGISDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIF
CCEEEECCCHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCHHEECCEEECCCCCCHHHHH
RMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAGYEVLMENRGALQRLLDLLKP
HHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHH
YLERNV
HHHCCC
>Mature Secondary Structure 
AFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNTSCPPPQGIFIHAASVG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHH
EVIAATPLVRQLQQDYPHLSITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFIN
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHH
FVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARSAHRYGKIKARLQTMWSQISL
HCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAQDHISGKRYAILGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAAS
HEECCCCCCCEEEEEECCHHHEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC
THNGEDEIILKSHRTLLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNE
CCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NTQVILGDSMGELMLMYGISDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIF
CCEEEECCCHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCHHEECCEEECCCCCCHHHHH
RMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAGYEVLMENRGALQRLLDLLKP
HHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHH
YLERNV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]