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The map label for this gene is pckA [H]

Identifier: 148826546

GI number: 148826546

Start: 1589959

End: 1591575

Strand: Direct

Name: pckA [H]

Synonym: CGSHiEE_08015

Alternate gene names: 148826546

Gene position: 1589959-1591575 (Clockwise)

Preceding gene: 148826545

Following gene: 148826547

Centisome position: 87.7

GC content: 39.46

Gene sequence:

>1617_bases
ATGACAGACTTAAATAAAGTGGTAAAAGAACTTGAAGCTCTTGGCATTTATGACGTAAAAGAAGTTGTTTACAATCCAAG
CTACGAGCAATTGTTTGAAGAAGAAACTAAACCAGGTTTAGAAGGCTTTGAAAAAGGTACTTTAACTACGACTGGTGCAG
TGGCAGTAGATACAGGTATCTTCACAGGTCGTTCTCCAAAAGATAAATATATCGTGTTAGATGAAAAAACCAAAGATACT
GTTTGGTGGACATCTGAAACAGCAAAAAACGACAACAAGCCAATGAACCAAGCTACATGGCAAAGCTTAAAAGACTTGGT
AACCAACCAACTTTCTCGTAAACGCTTATTTGTAGTTGATGGTTTCTGTGGTGCGAGCGAACACGACCGTATTGCAGTGC
GTATTGTCACTGAAGTAGCGTGGCAAGCACATTTTGTAAAAAATATGTTTATTCGCCCAACTGAAGAACAACTCAAAAAT
TTTGAACCAGATTTCGTTGTAATGAACGGTTCTAAAGTAACCAATCCAAACTGGAAAGAACAGGGTTTAAATTCAGAAAA
CTTTGTTGCTTTCAACTTGACTGAACGCATTCAATTAATCGGTGGTACTTGGTACGGCGGTGAAATGAAAAAAGGTATGT
TCTCAATCATGAACTACTTCCTACCACTTAAAGGTGTTGGTGCAATGCACTGCTCAGCTAACGTTGGTAAAGATGGTGAT
GTAGCAATCTTCTTCGGCTTATCTGGCACAGGTAAAACAACCCTTTCAACGGATCCAAAACGTGAATTAATCGGTGACGA
TGAACACGGCTGGGATGATGTTGGTATCTTTAACTTTGAAGGTGGTTGCTATGCGAAAACCATTCACCTTTCAGAAGAAA
ATGAACCAGATATTTACCGCGCTATCCGTCGCGATGCATTATTAGAAAACGTGGTTGTTCGTGCAGATGGTTCTGTTGAT
TTCGATGATGGTTCAAAAACAGAAAATACTCGCGTGTCTTACCCAATTTATCACATTGATAACATTGTAAAACCAGTTTC
TCGTGCAGGTCACGCAACTAAAGTGATTTTCTTAACTGCAGATGCATTTGGCGTATTACCACCAGTATCTAAATTGACAC
CAGAACAAACTAAATACTACTTCTTATCTGGTTTCACAGCGAAATTAGCAGGTACTGAACGTGGTATTACTGAACCAACG
CCAACTTTCTCAGCATGTTTCGGTGCTGCGTTCTTAACCCTTCACCCAACTCAATATGCAGAAGTGTTAGTAAAACGTAT
GCAAGCAGCAGGTGCTGAAGCTTACTTAGTGAATACTGGTTGGAATGGCACAGGCAAACGTATCTCAATCAAAGATACTC
GCGGAATCATTGATGCAATCTTAGATGGCTCAATTGAAAAAGCTGAAATGGGCGAATTACCAATCTTTAACTTAGCTATT
CCTAAAGCATTACTAGGTGTAGATTCTGCAATCTTAGATCCTCGCGATACTTACGCAGATAAAGCACAATGGCAATCAAA
AGCTGAAGACTTAGCAGGTCGTTTTGTGAAAAACTTTGTTAAATATGCAACTAACGAAGAAGGCAAAGCTTTAATTGCAG
CGGGTCCTAAAGCTTAA

Upstream 100 bases:

>100_bases
AAAATAGTTTAAAAATGAGATCTACTTAACTATTTTTTATATTTACATCCATTATAATAAACCACAGTTAAATTTTAATC
TTACATATTAGAGGTGATTT

Downstream 100 bases:

>100_bases
TTAAAAATACTAAAAATATTAACCGCACTTTGGGGAACTGAAGTGCGGTCATTTTTTATCTGTTTTCAAGCTGATAAATG
TATTCAAAAATTTAAAAAAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDT
VWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKN
FEPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYFLPLKGVGAMHCSANVGKDGD
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRADGSVD
FDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
PKALLGVDSAILDPRDTYADKAQWQSKAEDLAGRFVKNFVKYATNEEGKALIAAGPKA

Sequences:

>Translated_538_residues
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDT
VWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKN
FEPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYFLPLKGVGAMHCSANVGKDGD
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRADGSVD
FDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
PKALLGVDSAILDPRDTYADKAQWQSKAEDLAGRFVKNFVKYATNEEGKALIAAGPKA
>Mature_537_residues
TDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDTV
WWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNF
EPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYFLPLKGVGAMHCSANVGKDGDV
AIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRADGSVDF
DDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTP
TFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAIP
KALLGVDSAILDPRDTYADKAQWQSKAEDLAGRFVKNFVKYATNEEGKALIAAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=77.7358490566038, Blast_Score=871, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=504, Percent_Identity=46.6269841269841, Blast_Score=451, Evalue=1e-127,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59447; Mature: 59316

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGI
CCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCE
FTGRSPKDKYIVLDEKTKDTVWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVD
ECCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNFEPDFVVMNGSKVTNPNWKE
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYFLPLKGVGAMHCSANVGKDGD
HCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYR
EEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
AIRRDALLENVVVRADGSVDFDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTA
HHHHHHHHHHEEEEECCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEC
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLTLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHEEEECCHHHH
EVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEHHHH
PKALLGVDSAILDPRDTYADKAQWQSKAEDLAGRFVKNFVKYATNEEGKALIAAGPKA
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
>Mature Secondary Structure 
TDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGI
CCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCE
FTGRSPKDKYIVLDEKTKDTVWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVD
ECCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNFEPDFVVMNGSKVTNPNWKE
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYFLPLKGVGAMHCSANVGKDGD
HCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYR
EEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
AIRRDALLENVVVRADGSVDFDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTA
HHHHHHHHHHEEEEECCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEC
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLTLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHEEEECCHHHH
EVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEHHHH
PKALLGVDSAILDPRDTYADKAQWQSKAEDLAGRFVKNFVKYATNEEGKALIAAGPKA
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA