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The map label for this gene is rumB [H]

Identifier: 148826391

GI number: 148826391

Start: 1427141

End: 1428319

Strand: Direct

Name: rumB [H]

Synonym: CGSHiEE_07170

Alternate gene names: 148826391

Gene position: 1427141-1428319 (Clockwise)

Preceding gene: 148826390

Following gene: 148826397

Centisome position: 78.72

GC content: 35.2

Gene sequence:

>1179_bases
ATGATTGATTGTCAATACTATCAACAAAATGAATGTCGTTCTTGCCAATGGCTTGAAATACCTTATGACCAACAGATCGC
TGAAAAACAACATCATTTAAAACAACAACTTATCTCTCTTGATTGTTCTGACGTTCACTGGTTAGCTCCTTTTAAATCCA
ATGAACAAGGCTTTCGTAATAAAGCTAAAATGCTTGTTAGTGGTAGTGTGGAACGCCCAATACTTGGTATTTTAAAAAAT
CCTAATGATCCACAAAGTGCGATTGATTTGTGTGATTGTCCTCTTTATCCTGCTCATTTCTCAATAATTTTTTCAATCCT
CAAAGATTTTATTGGTCGCGCAGGTTTAGTACCTTATAACATTGCTAAACAAAAAGGCGAGCTTAAATATATTTTACTGA
CAGAAAGTATCGCAACAGGAAAATTAATGTTGCGTTTTGTGTTACGTACAGAAAATAAATTGCCGTTAATCCGTCGAGAA
TTACCAAAATTATTAGAAAAACTACCGCACTTAGAAGTGATTAGTATTAATTTGCAACCACAGCACGCGGCTATTTTAGA
GGGCGAACAAGAAATTTTTCTGACAGAACAACAATTTTTGCCTGAAAATTTTAACGGTATTCCACTCTTTATTCGTCCAC
AAGGCTTCTTTCAAACTAACCCTAAAGTGGCTGCAGGTTTATATGCAACCGCTCAACAATGGGTGGCTGAATTCCCTATT
TATAACCTGTGGGATTTATTTTGTGGCGTAGGGGGATTTGGCTTACATTGTGCTAAAGCGTTGCAGGAAAAATGGGGAAA
ACCCATTAAATTAACAGGAATTGAAATTTCTTCCTCTGCTATTTTGGCAGCCAGTCATTCTGCGAAAATATTAGGTTTAG
AACATGTAAATTTCCAATCTCTAAATGCAGCAAGCGTGATGGAAAATAAAAATGAAAATAAGCCAGATCTTGTGATTGTA
AATCCGCCGCGTCGTGGAATTGGAAAGCAATTAAGTGAATTTCTCAACCAAATTCAACCGCACTTTATCTTGTATTCCAG
CTGTAATGCCATGACAATGGGTAAAGATTTACAGCATCTAACTTGTTATAAACCATTGAAAATTCAACTTTTTGATATGT
TTCCGCAAACATCTCACTATGAAGTATTAGTTTTACTAGAAAGAAAAAAGATTAATTAA

Upstream 100 bases:

>100_bases
TCGATTGGAATGATCTGGTTTCAGATCTACGTTGGAAACTGAATTACCGAAATCTCGCAGATATTCAAATCCGTTGGCTA
GATATTCAGGCTGCAAAAAA

Downstream 100 bases:

>100_bases
GGTAAGCTAGAAAAGGCTAGGGAATTTGAAAGAAGGGTGGTGGAACTAAGCAGAATTGGTCTTAACAGCAAGTGGCTTAC
TGCACGCATCCCTGCGGGAT

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIDCQYYQQNECRSCQWLEIPYDQQIAEKQHHLKQQLISLDCSDVHWLAPFKSNEQGFRNKAKMLVSGSVERPILGILKN
PNDPQSAIDLCDCPLYPAHFSIIFSILKDFIGRAGLVPYNIAKQKGELKYILLTESIATGKLMLRFVLRTENKLPLIRRE
LPKLLEKLPHLEVISINLQPQHAAILEGEQEIFLTEQQFLPENFNGIPLFIRPQGFFQTNPKVAAGLYATAQQWVAEFPI
YNLWDLFCGVGGFGLHCAKALQEKWGKPIKLTGIEISSSAILAASHSAKILGLEHVNFQSLNAASVMENKNENKPDLVIV
NPPRRGIGKQLSEFLNQIQPHFILYSSCNAMTMGKDLQHLTCYKPLKIQLFDMFPQTSHYEVLVLLERKKIN

Sequences:

>Translated_392_residues
MIDCQYYQQNECRSCQWLEIPYDQQIAEKQHHLKQQLISLDCSDVHWLAPFKSNEQGFRNKAKMLVSGSVERPILGILKN
PNDPQSAIDLCDCPLYPAHFSIIFSILKDFIGRAGLVPYNIAKQKGELKYILLTESIATGKLMLRFVLRTENKLPLIRRE
LPKLLEKLPHLEVISINLQPQHAAILEGEQEIFLTEQQFLPENFNGIPLFIRPQGFFQTNPKVAAGLYATAQQWVAEFPI
YNLWDLFCGVGGFGLHCAKALQEKWGKPIKLTGIEISSSAILAASHSAKILGLEHVNFQSLNAASVMENKNENKPDLVIV
NPPRRGIGKQLSEFLNQIQPHFILYSSCNAMTMGKDLQHLTCYKPLKIQLFDMFPQTSHYEVLVLLERKKIN
>Mature_392_residues
MIDCQYYQQNECRSCQWLEIPYDQQIAEKQHHLKQQLISLDCSDVHWLAPFKSNEQGFRNKAKMLVSGSVERPILGILKN
PNDPQSAIDLCDCPLYPAHFSIIFSILKDFIGRAGLVPYNIAKQKGELKYILLTESIATGKLMLRFVLRTENKLPLIRRE
LPKLLEKLPHLEVISINLQPQHAAILEGEQEIFLTEQQFLPENFNGIPLFIRPQGFFQTNPKVAAGLYATAQQWVAEFPI
YNLWDLFCGVGGFGLHCAKALQEKWGKPIKLTGIEISSSAILAASHSAKILGLEHVNFQSLNAASVMENKNENKPDLVIV
NPPRRGIGKQLSEFLNQIQPHFILYSSCNAMTMGKDLQHLTCYKPLKIQLFDMFPQTSHYEVLVLLERKKIN

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=27.8606965174129, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=27.8606965174129, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1787083, Length=388, Percent_Identity=54.639175257732, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789148, Length=384, Percent_Identity=25.5208333333333, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24666579, Length=427, Percent_Identity=24.824355971897, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 44622; Mature: 44622

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDCQYYQQNECRSCQWLEIPYDQQIAEKQHHLKQQLISLDCSDVHWLAPFKSNEQGFRN
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
KAKMLVSGSVERPILGILKNPNDPQSAIDLCDCPLYPAHFSIIFSILKDFIGRAGLVPYN
HHHEEECCCCCCCHHHEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
IAKQKGELKYILLTESIATGKLMLRFVLRTENKLPLIRRELPKLLEKLPHLEVISINLQP
HHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCC
QHAAILEGEQEIFLTEQQFLPENFNGIPLFIRPQGFFQTNPKVAAGLYATAQQWVAEFPI
CHHHEECCCCCEEEEHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCH
YNLWDLFCGVGGFGLHCAKALQEKWGKPIKLTGIEISSSAILAASHSAKILGLEHVNFQS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEECCCCCC
LNAASVMENKNENKPDLVIVNPPRRGIGKQLSEFLNQIQPHFILYSSCNAMTMGKDLQHL
CCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEECCCCCHHH
TCYKPLKIQLFDMFPQTSHYEVLVLLERKKIN
HCCCCCEEEEEECCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MIDCQYYQQNECRSCQWLEIPYDQQIAEKQHHLKQQLISLDCSDVHWLAPFKSNEQGFRN
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
KAKMLVSGSVERPILGILKNPNDPQSAIDLCDCPLYPAHFSIIFSILKDFIGRAGLVPYN
HHHEEECCCCCCCHHHEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCH
IAKQKGELKYILLTESIATGKLMLRFVLRTENKLPLIRRELPKLLEKLPHLEVISINLQP
HHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCC
QHAAILEGEQEIFLTEQQFLPENFNGIPLFIRPQGFFQTNPKVAAGLYATAQQWVAEFPI
CHHHEECCCCCEEEEHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCH
YNLWDLFCGVGGFGLHCAKALQEKWGKPIKLTGIEISSSAILAASHSAKILGLEHVNFQS
HHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEECCCCCC
LNAASVMENKNENKPDLVIVNPPRRGIGKQLSEFLNQIQPHFILYSSCNAMTMGKDLQHL
CCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEECCCCCHHH
TCYKPLKIQLFDMFPQTSHYEVLVLLERKKIN
HCCCCCEEEEEECCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA