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The map label for this gene is smf [H]

Identifier: 148826367

GI number: 148826367

Start: 1403746

End: 1404867

Strand: Direct

Name: smf [H]

Synonym: CGSHiEE_07040

Alternate gene names: 148826367

Gene position: 1403746-1404867 (Clockwise)

Preceding gene: 148826362

Following gene: 148826368

Centisome position: 77.43

GC content: 40.82

Gene sequence:

>1122_bases
ATGAATGACATTACCTACACATTACTCCGTTTAATGCAAGTGCCTAAACTTGGCGGCGTTGGCATTGATAAAATTTTGTC
CAATATTACCTTAAATGAATTACTTAATTATGATGATGTTGCTTTCCGACAAATGGGGTGGGGAGCCATCCAAATTCGCC
GTTGGTTCAAGCCTGAAGCAAAATTTATTGAGCCTGCACTTGTGTGGTCACAAAAAGAGGGAAATCATTTAGTGAATTAT
TTTTCCCCTTTTTATCCCTTTTTATTGAAACAGACCGCAAGTTTTCCGCCACTATTATTTGTAAAAGGAAATTTAACCGC
ACTTTCACAACGCCAAATGGCAATGGTTGGAAGCCGTTATTGCACGACTTACGGAGAATATTGGGCAAAACATTTTGCGA
CAGAACTTTCATTAGCTGGCTTTACGATTACTAGTGGACTTGCATTAGGAATTGATGGACATTGCCATCAAGCTGTCGTC
AATATTCAAGGACAAACTATTGCGGTTTTAGGCAGTGGATTAGAACAAATCTATCCCTCTAAACATCAAAGATTATCGGC
ACAAATCATCGAAAATAATGGCGCATTAGTCTCTGAGTTCTTACCAAACCAAGCCCCCATTGCCGCTAATTTCCCACATC
GTAATCGGATTATTAGCGGGCTTTCAGTCGGCACGCTTGTTGTTGAAGCCACAGAAAAAAGCGGCTCGCTTATTACTGCG
CGATATGCGTTGGAACAAAACCGAGAGGTTTTTGCTGTGCCGGGAAACATTCAAAATAAATCAAGTCAAGGTTGTCATCG
CCTAATTAAACAAGGTGCAATGTTGGTAGAAAATGCTAAAGATATTTTGGAAACCCTCTACCAACATTCTATCCATAGCC
AAACTGAAATTGATTTTGACCAGATCGCCGTGCCTAATTACACGCCACCACCTGATCCTCGCCGATTAGTAGAAGCACCA
AGCCACCCGAAACTTTATTCGCGTATTGGTTACACGCCAGTGAGCATTGATGATTTAGCGGAAGAATTTAATTTAAGCGT
CGATGTTTTGCTTGTTCAGTTGCTCGATCTTGAGTTGCAAGATCTCATCATGAGTGAAAATGGGTTATATAAACGCGTTT
AG

Upstream 100 bases:

>100_bases
AAAAAGAATTTTTGCGATCTGCATCGCAAAATGCTTTTAAATTAATCGTTTCTCTCCTTCGTTTTTTCCATACTAGATTT
TCAAATAAAAAGGAAAAAAT

Downstream 100 bases:

>100_bases
TGTAAATTTTTATGCTAAGATACGCCCATTCTTTTTTCATAAAAGGACGATTATGTTAGCGATTATCTCCCCTGCCAAAA
CTCTTGATTTTGAAAGTGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEAKFIEPALVWSQKEGNHLVNY
FSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRYCTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVV
NIQGQTIAVLGSGLEQIYPSKHQRLSAQIIENNGALVSEFLPNQAPIAANFPHRNRIISGLSVGTLVVEATEKSGSLITA
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFDQIAVPNYTPPPDPRRLVEAP
SHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQDLIMSENGLYKRV

Sequences:

>Translated_373_residues
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEAKFIEPALVWSQKEGNHLVNY
FSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRYCTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVV
NIQGQTIAVLGSGLEQIYPSKHQRLSAQIIENNGALVSEFLPNQAPIAANFPHRNRIISGLSVGTLVVEATEKSGSLITA
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFDQIAVPNYTPPPDPRRLVEAP
SHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQDLIMSENGLYKRV
>Mature_373_residues
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEAKFIEPALVWSQKEGNHLVNY
FSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRYCTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVV
NIQGQTIAVLGSGLEQIYPSKHQRLSAQIIENNGALVSEFLPNQAPIAANFPHRNRIISGLSVGTLVVEATEKSGSLITA
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFDQIAVPNYTPPPDPRRLVEAP
SHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQDLIMSENGLYKRV

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=369, Percent_Identity=43.9024390243902, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 41639; Mature: 41639

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCHHHHHHCCCCCEEHHHHCCCCH
KFIEPALVWSQKEGNHLVNYFSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRY
HHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
CTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVVNIQGQTIAVLGSGLEQIYPS
HHHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCEEEEEECCCEEEEECCCHHHHCCC
KHQRLSAQIIENNGALVSEFLPNQAPIAANFPHRNRIISGLSVGTLVVEATEKSGSLITA
HHHHHHHEEEECCCCCHHHHCCCCCCEECCCCHHHHHHCCCCEEEEEEEEECCCCCEEEE
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFD
EEHHHCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QIAVPNYTPPPDPRRLVEAPSHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQ
HEECCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
DLIMSENGLYKRV
HHHHCCCCCCCCC
>Mature Secondary Structure
MNDITYTLLRLMQVPKLGGVGIDKILSNITLNELLNYDDVAFRQMGWGAIQIRRWFKPEA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCHHHHHHCCCCCEEHHHHCCCCH
KFIEPALVWSQKEGNHLVNYFSPFYPFLLKQTASFPPLLFVKGNLTALSQRQMAMVGSRY
HHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
CTTYGEYWAKHFATELSLAGFTITSGLALGIDGHCHQAVVNIQGQTIAVLGSGLEQIYPS
HHHHHHHHHHHHHHHHHHCCEEECCCEEECCCCCCCEEEEEECCCEEEEECCCHHHHCCC
KHQRLSAQIIENNGALVSEFLPNQAPIAANFPHRNRIISGLSVGTLVVEATEKSGSLITA
HHHHHHHEEEECCCCCHHHHCCCCCCEECCCCHHHHHHCCCCEEEEEEEEECCCCCEEEE
RYALEQNREVFAVPGNIQNKSSQGCHRLIKQGAMLVENAKDILETLYQHSIHSQTEIDFD
EEHHHCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
QIAVPNYTPPPDPRRLVEAPSHPKLYSRIGYTPVSIDDLAEEFNLSVDVLLVQLLDLELQ
HEECCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
DLIMSENGLYKRV
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]