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The map label for this gene is recF [H]

Identifier: 148826361

GI number: 148826361

Start: 1391524

End: 1392606

Strand: Direct

Name: recF [H]

Synonym: CGSHiEE_07010

Alternate gene names: 148826361

Gene position: 1391524-1392606 (Clockwise)

Preceding gene: 148826360

Following gene: 148826362

Centisome position: 76.75

GC content: 39.7

Gene sequence:

>1083_bases
ATGTCATTATGCCAATGCGTTTATAATATGGCGATTTCTCGTTTACTGGTCGAAAAGTTTCGTAACTTAACAGCAGTGGA
TCTTGATTTTGATCCCTGCTTTAACTTTTTAATCGGCAACAACGGCAGCGGAAAAACCAGCTTATTAGAAGCCATTTTTT
ATCTTGGTCACGGACGTTCATTTAAAAGTGCGGTCACAAATCGCATCATTTCTTACGACGAACCGCACTTTACCCTATTT
GGACAAATCCAAGAAAGCCAACATCAATGGTCTGTTGGCTTACAAAAACTGCGTCAAGGCAACACCTTAGTAAAAATTAA
CGGCGAAGATGGCAATAAAATTTCCGATCTAGCCCATCTTTTACCAATGCAATTAATCACACCAGAGGGCTTAACCTTAC
TCAACGGTGGGCCAAGTTATCGCCGTGCGTTTTTAGATTGGGGATTATTCCACCACCAAACAAGTTTCTATTCCGCTTGG
AGCAATCTCAACCGATTACTTAAACAACGAAATGCCGCACTCGCACAAAATCAGCCTTACTCTGCCATAAAAATTTGGGA
CGTTGAACTTGCCAAATTTGCCCACCAAGTAAGTCAATGGCGTGCCGAATATGCAGAAGCTCTCCGCCCTGAAATTGAAC
AAACTTGCCAACTTTTTCTGCCTGAATTAGAAATTAATGTCAGCTTTCATCAAGGGTGGGAAAAAAATGCTGATTATTAT
GAAATTCTTCAACAAAATTTTGAACGAGATCGCGCCTTAAATTACACCTTTTCTGGCCCTCAAAAAGCGGATTTTCGTTT
CAAAGCACAAGGATTGCCTGTTGAAGATGTGCTTTCTCGTGGACAACTTAAACTTTTAATGTGTGCATTAAGACTAGCAC
AGGGCGAGCATTTAATGAAAGAAAAGCAACGCCATTGTATTTTTTTAATCGATGATTTTGCCTCGGAATTAGATCAATAC
AAACGCGCCTTGTTGGCAGAACGATTACAACAAAGTGGCTCACAAGTGTTTGTTACCGCCATCACTAAAAGACAACTAAA
AGAAATGCAAGTGGAAAACAAAAAAATAGTTTTCTGTTCATAA

Upstream 100 bases:

>100_bases
GATGTGCTGAATGCACTTAAATGTAATCAAGTGCGAATGCGATTAACTGATGCGTTCTCCAGTTGCTTAATTGAAAACTG
TGAAGACAGCAGCAGCGAAT

Downstream 100 bases:

>100_bases
TGGCATCATTAACGCCTTAAATTAGCCAAAAGTGCGGTGGAAAATACGCCAATTTTTTAAAAAATATCAAAAATCATTAT
CATTACCTCGTAAATGTAAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSLCQCVYNMAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLF
GQIQESQHQWSVGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAW
SNLNRLLKQRNAALAQNQPYSAIKIWDVELAKFAHQVSQWRAEYAEALRPEIEQTCQLFLPELEINVSFHQGWEKNADYY
EILQQNFERDRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQY
KRALLAERLQQSGSQVFVTAITKRQLKEMQVENKKIVFCS

Sequences:

>Translated_360_residues
MSLCQCVYNMAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLF
GQIQESQHQWSVGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAW
SNLNRLLKQRNAALAQNQPYSAIKIWDVELAKFAHQVSQWRAEYAEALRPEIEQTCQLFLPELEINVSFHQGWEKNADYY
EILQQNFERDRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQY
KRALLAERLQQSGSQVFVTAITKRQLKEMQVENKKIVFCS
>Mature_359_residues
SLCQCVYNMAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRSFKSAVTNRIISYDEPHFTLFG
QIQESQHQWSVGLQKLRQGNTLVKINGEDGNKISDLAHLLPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWS
NLNRLLKQRNAALAQNQPYSAIKIWDVELAKFAHQVSQWRAEYAEALRPEIEQTCQLFLPELEINVSFHQGWEKNADYYE
ILQQNFERDRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMKEKQRHCIFLIDDFASELDQYK
RALLAERLQQSGSQVFVTAITKRQLKEMQVENKKIVFCS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=347, Percent_Identity=56.7723342939481, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41463; Mature: 41332

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLCQCVYNMAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRS
CCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCC
FKSAVTNRIISYDEPHFTLFGQIQESQHQWSVGLQKLRQGNTLVKINGEDGNKISDLAHL
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
LPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQRNAALAQNQPY
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAIKIWDVELAKFAHQVSQWRAEYAEALRPEIEQTCQLFLPELEINVSFHQGWEKNADYY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
EILQQNFERDRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMK
HHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHH
EKQRHCIFLIDDFASELDQYKRALLAERLQQSGSQVFVTAITKRQLKEMQVENKKIVFCS
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SLCQCVYNMAISRLLVEKFRNLTAVDLDFDPCFNFLIGNNGSGKTSLLEAIFYLGHGRS
CHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCC
FKSAVTNRIISYDEPHFTLFGQIQESQHQWSVGLQKLRQGNTLVKINGEDGNKISDLAHL
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
LPMQLITPEGLTLLNGGPSYRRAFLDWGLFHHQTSFYSAWSNLNRLLKQRNAALAQNQPY
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAIKIWDVELAKFAHQVSQWRAEYAEALRPEIEQTCQLFLPELEINVSFHQGWEKNADYY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
EILQQNFERDRALNYTFSGPQKADFRFKAQGLPVEDVLSRGQLKLLMCALRLAQGEHLMK
HHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCHHHHH
EKQRHCIFLIDDFASELDQYKRALLAERLQQSGSQVFVTAITKRQLKEMQVENKKIVFCS
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA