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The map label for this gene is dnaN [H]

Identifier: 148826360

GI number: 148826360

Start: 1390449

End: 1391549

Strand: Direct

Name: dnaN [H]

Synonym: CGSHiEE_07005

Alternate gene names: 148826360

Gene position: 1390449-1391549 (Clockwise)

Preceding gene: 148826359

Following gene: 148826361

Centisome position: 76.69

GC content: 39.06

Gene sequence:

>1101_bases
ATGCAATTTAGTATTTCAAGAGAAAATTTATTAAAACCGTTGCAACAAGTATGTGGCGTATTGAGTCATCGCCCAAATAT
TCCTGTATTAAACAACGTATTATTGCAAATCGAAGACAATCGTCTGACTATCACAGGGACAGATTTAGAAGTTGAACTCT
CTAGTCAAACTCAGCTTTCATCATCTTCTGAAAATGGCACTTTTACCATTCCAGCAAAAAAATTCTTAGATATTTGCCGC
ACTTTATCTGATGATTCAGAAATTACGGTGACTTTTGAACAAGATCGTGCATTAGTGCAGTCTGGGCGTAGCCGTTTTAC
ACTAGCAACCCAACCTGCCGAAGAATATCCCAACCTCACAGATTGGCAGTCTGAAGTGGATTTTGAACTACCGCAAAATA
CCTTACGCCGTTTAATTGAAGCCACTCAATTTTCTATGGCAAACCAAGATGCGCGCTATTTCTTAAATGGTATGAAGTTT
GAAACAGAAGGCAATTTATTGCGAACGGTGGCAACAGATGGTCATAGACTTGCGGTGTGTACCATTTCCCTTGAACAAGA
ATTACAAAATCATTCTGTGATTTTACCTCGTAAAGGCGTGTTAGAGCTGGTTCGTTTATTAGAAACAAACGATGAACCAG
CACGTTTACAAATTGGCACCAACAATTTACGTGTCCATTTAAAAAACACGGTATTTACATCAAAATTAATTGATGGTCGC
TTTCCTGACTATCGCCGAGTATTGCCTCGTAATGCCACAAAAATTGTCGAAGGCAGTTGGGAAATGCTGAAACAGGCTTT
TATTCGTGCGTCCATTTTATCTAATGAACGCGCACGCAGCGTGCGTTTAAGCTTAAAGGAAAACCAGCTTAAAATTACTG
CCTCAAACACAGAACACGAAGAAGCAGAAGAAATTGTCGATGTCAATTACAATGGCGAAGAATTAGAAGTGGGCTTTAAT
GTCACCTATATTCTTGATGTGCTGAATGCACTTAAATGTAATCAAGTGCGAATGCGATTAACTGATGCGTTCTCCAGTTG
CTTAATTGAAAACTGTGAAGACAGCAGCAGCGAATATGTCATTATGCCAATGCGTTTATAA

Upstream 100 bases:

>100_bases
CGCTTGCCGAGAAGTACCAAAATTCCGTGAGCAAGACAATAGCATTCAAGAAGATTGGGCGAATTTGATCCGCACTTTAT
CCGCATAAAAGGAGAACGTA

Downstream 100 bases:

>100_bases
TATGGCGATTTCTCGTTTACTGGTCGAAAAGTTTCGTAACTTAACAGCAGTGGATCTTGATTTTGATCCCTGCTTTAACT
TTTTAATCGGCAACAACGGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQFSISRENLLKPLQQVCGVLSHRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGSWEMLKQAFIRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMRLTDAFSSCLIENCEDSSSEYVIMPMRL

Sequences:

>Translated_366_residues
MQFSISRENLLKPLQQVCGVLSHRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGSWEMLKQAFIRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMRLTDAFSSCLIENCEDSSSEYVIMPMRL
>Mature_366_residues
MQFSISRENLLKPLQQVCGVLSHRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLSSSSENGTFTIPAKKFLDICR
TLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLTDWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
FPDYRRVLPRNATKIVEGSWEMLKQAFIRASILSNERARSVRLSLKENQLKITASNTEHEEAEEIVDVNYNGEELEVGFN
VTYILDVLNALKCNQVRMRLTDAFSSCLIENCEDSSSEYVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=62.2950819672131, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41658; Mature: 41658

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSISRENLLKPLQQVCGVLSHRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEHHHHHCCC
FPDYRRVLPRNATKIVEGSWEMLKQAFIRASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCHHEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMRLTDAFSSCLIENCEDSSSEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IMPMRL
EEEECC
>Mature Secondary Structure
MQFSISRENLLKPLQQVCGVLSHRPNIPVLNNVLLQIEDNRLTITGTDLEVELSSQTQLS
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCEEEEECCCCCCC
SSSENGTFTIPAKKFLDICRTLSDDSEITVTFEQDRALVQSGRSRFTLATQPAEEYPNLT
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEEECCHHHCCCCC
DWQSEVDFELPQNTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TISLEQELQNHSVILPRKGVLELVRLLETNDEPARLQIGTNNLRVHLKNTVFTSKLIDGR
EEEHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEHHHHHCCC
FPDYRRVLPRNATKIVEGSWEMLKQAFIRASILSNERARSVRLSLKENQLKITASNTEHE
CCHHHHHCCCCCHHEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEIVDVNYNGEELEVGFNVTYILDVLNALKCNQVRMRLTDAFSSCLIENCEDSSSEYV
HHHHEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IMPMRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]