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The map label for this gene is rnhB [H]

Identifier: 148826326

GI number: 148826326

Start: 1343070

End: 1343663

Strand: Direct

Name: rnhB [H]

Synonym: CGSHiEE_06785

Alternate gene names: 148826326

Gene position: 1343070-1343663 (Clockwise)

Preceding gene: 148826325

Following gene: 148826327

Centisome position: 74.08

GC content: 42.76

Gene sequence:

>594_bases
ATGTTTGAATATCCACAAGGCTATAAATTGATCGCTGGCGTTGATGAAGTTGGGCGAGGCCCTCTAGTTGGGGCGGTTGT
CACGGCAGCCGTCATTTTAGATCCTCATAATCCAATTGAAGGATTAGCTGATTCTAAAAAACTTTCAGAAAAAAAACGTT
TAGCCCTTGCTGACGAAATTAAAGAAAAAGCCCTTGCTTGGGCATTAGGTCGAGCTGAAGCTGACGAAATTGACGAGATA
AATATTCTGCAAGCCTCCTTGCTGGCAATGACTCGAGCGGTAAAATCTTTAAAAATTCAACCGCACTTTGTGTTGGTTGA
TGGCAATAAGATCCCAAAAGATTTAGATATTCCAGCGCAAGCCGTCGTAAAAGGCGATAGTATTGTGGCTGAAATCAGTG
CAGCCTCTATTTTGGCGAAAGTGGCACGAGACCAAGAAATGGAAGAATTAGACAAGCAATATCCAGAATACGCTTTTGCC
AAGCATAAAGGCTATCCGACCAAATTACATTTGGAAAAATTAGCTGAGCTTGGCGCGTTACCGCAACATCGTCGTAGCTT
TGCCCCAGTGAAAAAAGCACTAGAACAGTTTTAG

Upstream 100 bases:

>100_bases
GTTTTAATTCAGCACTTCACGGATTTACACCAAAAAATTCAATGCAATGCCGATAAGCAAGCGGCTCAAGCCGTCATTGA
TTTATTAGAAGGAACAGAAA

Downstream 100 bases:

>100_bases
AGGTTTAACAAAGGCTGTGTTTTAATACTGTGTTGATGCTTCACTAAAATTTGATACAGATAAACAAAATTTTTTGACTT
CTGTGAGTCAAAGAAAACAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MFEYPQGYKLIAGVDEVGRGPLVGAVVTAAVILDPHNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEADEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLDIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
KHKGYPTKLHLEKLAELGALPQHRRSFAPVKKALEQF

Sequences:

>Translated_197_residues
MFEYPQGYKLIAGVDEVGRGPLVGAVVTAAVILDPHNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEADEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLDIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
KHKGYPTKLHLEKLAELGALPQHRRSFAPVKKALEQF
>Mature_197_residues
MFEYPQGYKLIAGVDEVGRGPLVGAVVTAAVILDPHNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEADEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLDIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
KHKGYPTKLHLEKLAELGALPQHRRSFAPVKKALEQF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=193, Percent_Identity=71.5025906735751, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21583; Mature: 21583

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEYPQGYKLIAGVDEVGRGPLVGAVVTAAVILDPHNPIEGLADSKKLSEKKRLALADEI
CCCCCCCCEEEECHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KEKALAWALGRAEADEIDEINILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLDIPAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHH
AVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFAKHKGYPTKLHLEKLAELGAL
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCC
PQHRRSFAPVKKALEQF
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEYPQGYKLIAGVDEVGRGPLVGAVVTAAVILDPHNPIEGLADSKKLSEKKRLALADEI
CCCCCCCCEEEECHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KEKALAWALGRAEADEIDEINILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLDIPAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHH
AVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFAKHKGYPTKLHLEKLAELGAL
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCC
PQHRRSFAPVKKALEQF
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA