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The map label for this gene is cca

Identifier: 148826131

GI number: 148826131

Start: 1135624

End: 1136874

Strand: Direct

Name: cca

Synonym: CGSHiEE_05680

Alternate gene names: 148826131

Gene position: 1135624-1136874 (Clockwise)

Preceding gene: 148826130

Following gene: 148826132

Centisome position: 62.64

GC content: 38.85

Gene sequence:

>1251_bases
ATGAAAATTTATTTGGTTGGTGGGGCTGTTCGCGATCAATTATTGGGGTTACCAGTAAAAGATCGTGATTGGATTGTGGT
AGGCGCAGACCACGCTACGCTACTCTCTCTCGGTTATCAGCAAGTAGGCAAAGATTTTCCGGTTTTTCTCAATCCTAAAA
CAAAAGAAGAATATGCGCTTGCCCGAACAGAACGTAAATCTAGCGCAGGTTACACTGGCTTTATTTGTGATTTCTCCCCA
ACAATCACATTAGAACAAGATTTAATTCGACGAGATCTCACTATTAACGCGATGGCTCAAAGCGAAGACGGTGAAATTAT
CGATCCTTATGGCGGAAAACAAGATTTAGAAAATCGAATTTTACGCCATATTTCTCCCGCTTTTTCAGAAGATCCTTTAC
GAGTATTACGCGTGGCACGCTTTGCAGCCCGTTATCATTCTCTCGGTTTTAAAATCGCCTCGGAAACATTAGCTCTAATG
ACAGAACTTGCCCTATCGGGAGAATTACAACATCTCACGGCGGAACGCGTTTGGCTAGAAACAGAAAAAGCCTTAAACGA
AAAAAATCCTGAAATTTATTTTGAAACCTTACATAAAACAGGCGCATTAAGCGTTTTATTTTCTGAAATCGATGCCCTTC
ATGGGGTGCCAAATCCAGTAAAACATCATCCCGAAGTGGATAGTTTTATTCATACCATGTTGGTATTAAAACAAGCGGTT
AATTTGACTGAAAATAACCCTATCCTAAATAAAAGTGCGGTTCGTTTTGCGGCTATTTGTCATGATCTTGGCAAGGCTCT
TACACCTCAAAATATTCTTCCTCATCATTATGGGCATGAACAAGCTGGCATAAAACCGACTAGATCATTGTGCAAACGCT
TGAAGGTTCCAAGTTATTTTCAAGAACTTGCAGAACTGACTTGTGAATTTCACACGCATATTCACAAAGCCTTTGAGCTT
CGAGCTGAAACCGTCATAACGCTATTTAATCGTTTTGATGTGTGGAGAAAACCACAACGCTTTCAAGAATTTCTACAAGT
ATGTTTAGCTGATACTCGAGGTAGAACAGGTTTTGAAACTAAAGACTATCCACAAATAGATTACATCAATCAATTATTAC
ACTCTGCAAATGAAGTAGATGTACAGCAAGTTATTGCTGATGGATTTGAGAAACAAGCAATCAAAAATGAATTGACGAAA
CGAAGAATCTTAGCGGTTAAACAAACAAAAACGAATTACCCAACAAATTAA

Upstream 100 bases:

>100_bases
TTATGGCGGTTCAGTATTGGGCGTAGGCTTACTCTTCGGCTTGCTTATTCCTTATGTATTACCAAAACGTCGCCGTCGTG
ATGGTTGGGCATAATCACGA

Downstream 100 bases:

>100_bases
CAAATAAAAGTAACTTTACTCAATTAAACTAAATCCTTAAAATATGCGAGCTTATTAACTTGATGAATAATATGAAAACG
TTTAAATTTCTCACCGCACT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADHATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
TELALSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHSANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Sequences:

>Translated_416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADHATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
TELALSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHSANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN
>Mature_416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADHATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSAGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
TELALSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFETKDYPQIDYINQLLHSANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=195, Percent_Identity=30.2564102564103, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=58.5365853658537, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87081691, Length=178, Percent_Identity=34.2696629213483, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=238, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281360112, Length=372, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357337, Length=372, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_HAEIE (A5UCK0)

Other databases:

- EMBL:   CP000671
- RefSeq:   YP_001290884.1
- ProteinModelPortal:   A5UCK0
- SMR:   A5UCK0
- STRING:   A5UCK0
- GeneID:   5226215
- GenomeReviews:   CP000671_GR
- KEGG:   hip:CGSHiEE_05680
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   PRK10885
- BioCyc:   HINF374930:CGSHIEE_05680-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 47293; Mature: 47293

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADHATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSAGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMTELALSGELQHLTAERVWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHSANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADHATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSAGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMTELALSGELQHLTAERVWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKPQRFQEFLQVCLADTRGRTGFET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHSANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA