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The map label for this gene is lolB [H]

Identifier: 148826132

GI number: 148826132

Start: 1136946

End: 1137566

Strand: Direct

Name: lolB [H]

Synonym: CGSHiEE_05685

Alternate gene names: 148826132

Gene position: 1136946-1137566 (Clockwise)

Preceding gene: 148826131

Following gene: 148826133

Centisome position: 62.71

GC content: 36.39

Gene sequence:

>621_bases
ATGAAAACGTTTAAATTTCTCACCGCACTTTTTGCCACTGCGATTCTAACCGCTTGCACATTAGATATGGAACGTCCTAC
TAATGTACAATACATTGATAAAACGGATGCGATATGGCAACAACATTTACAAAAGATTCAAAAAATTCAATCCTATCAAG
CCAAAGGACAAATTGGTTATATCAGTCCTACAGAACGTTTTTCTAGCCGCTTTGAATGGCAATACCAAAATCCAAAATCT
TATACACTTAAACTCTATTCTTTAATCAGTAAATCTACCCTGTTGATTCAAATGCATCAAAGTGGGATGACAATTTCCGA
TAACAATGGCAATCAACAATCTGCAGCCAATGCTAAACTACTATTACAAGAGATCATTGGCATGGATGTGCCATTAGAAC
ATTTAGCTTATTGGTTGAAAGGCCAACCAGCAATGAACGCGGATTATCAAGTGGGTACCAACCATTTACTTGGTGCATTT
ACTTATCATGTGGATGGTTCTCAATGGACGGCAGATTATCTAACCTATCACTCAAATAATTCGATGCCTGAAAATATTTT
GCTGAAAAATGACAGCACAAAACAAACCTTAAAAATTCGCGTAGACGAGTGGATTTACTAA

Upstream 100 bases:

>100_bases
AAACAAAAACGAATTACCCAACAAATTAACAAATAAAAGTAACTTTACTCAATTAAACTAAATCCTTAAAATATGCGAGC
TTATTAACTTGATGAATAAT

Downstream 100 bases:

>100_bases
TGAATACATTAATGAAATCACATCAATTTTCTACCGCACTTTGCCAAAATACAACACAATCAAATGGGCAGCCATTACGC
TTCCCTAGCCCTGCCAAACT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKTFKFLTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQNPKS
YTLKLYSLISKSTLLIQMHQSGMTISDNNGNQQSAANAKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAF
TYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY

Sequences:

>Translated_206_residues
MKTFKFLTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQNPKS
YTLKLYSLISKSTLLIQMHQSGMTISDNNGNQQSAANAKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAF
TYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY
>Mature_206_residues
MKTFKFLTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGYISPTERFSSRFEWQYQNPKS
YTLKLYSLISKSTLLIQMHQSGMTISDNNGNQQSAANAKLLLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAF
TYHVDGSQWTADYLTYHSNNSMPENILLKNDSTKQTLKIRVDEWIY

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=213, Percent_Identity=27.2300469483568, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 23711; Mature: 23711

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFKFLTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEE
ISPTERFSSRFEWQYQNPKSYTLKLYSLISKSTLLIQMHQSGMTISDNNGNQQSAANAKL
CCCHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCHHHHHHH
LLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAFTYHVDGSQWTADYLTYHSNN
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHEEECCCC
SMPENILLKNDSTKQTLKIRVDEWIY
CCCCEEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKTFKFLTALFATAILTACTLDMERPTNVQYIDKTDAIWQQHLQKIQKIQSYQAKGQIGY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEE
ISPTERFSSRFEWQYQNPKSYTLKLYSLISKSTLLIQMHQSGMTISDNNGNQQSAANAKL
CCCHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCHHHHHHH
LLQEIIGMDVPLEHLAYWLKGQPAMNADYQVGTNHLLGAFTYHVDGSQWTADYLTYHSNN
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHEEECCCC
SMPENILLKNDSTKQTLKIRVDEWIY
CCCCEEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA