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The map label for this gene is truB [H]

Identifier: 148825873

GI number: 148825873

Start: 868109

End: 869029

Strand: Direct

Name: truB [H]

Synonym: CGSHiEE_04190

Alternate gene names: 148825873

Gene position: 868109-869029 (Clockwise)

Preceding gene: 148825872

Following gene: 148825874

Centisome position: 47.88

GC content: 42.89

Gene sequence:

>921_bases
ATGTCTAGACCTCGTAAACGCTGGCGTGATGTTGATGGTGTGTTTTTGTTGGATAAACCACAAGGCATGTCATCAAATGA
CATTATGCAAAAGGTGAAGCGTTTATTTCAAGCGAATAAAGCAGGGCATACAGGTGCGCTCGATCCGTTGGCTACGGGAA
TGTTGCCAATTTGCTTGGGTGAAGCAACTAAGTTTTCACAATTTTTGCTTGATGCCGATAAGCGTTATCTTGTTACGGCA
AAATTAGGCGAACGTACCGATACTTCCGATGCGGAAGGGCAAGTCGTGGAAACTCGTGAAGTTCATGTTGAAACTCCGCA
AATTTTGACCGCACTTGAACAATTTCGTGGCGATATTTTGCAAGTGCCGACCATGTTTTCCGCACTAAAACATAACGGTA
AACCGCTTTATGAATATGCGCGTCAAGGTATTACGGTTGAGCGTGAGGCACGTCCAATTACGATTTTTGAACTTAATTTT
ATTGAATATCACGCACCTTTTTTAACCTTAGAAGTACATTGTTCAAAAGGCACTTATATTCGCACCTTAGTCGATGATTT
GGGTGAAGTGCTAGGTTGTGGCGCGCATGTGACAATGTTACGCCGCACAGCCGTAGCGGATTACCCCGTAGCAGAAATGA
TGCCAATCAATGAGTTACAACTGCTCGCTGAAAGTTTTCCATTAAGTGAATTGGATCGTCTATTACTTCCAACCGATACC
GCAGTAAGTAAATTGCCCGCTTTGCATTTGGATGTAGAGCAGAGTAAAGCCATTGGTTTTGGTCAGCGAGTGAAATTTGC
TAATGAACAGCAATTAAGCGGTCAAGTGCGGTTATTTTCAGCGGAGAATTTATTCTTAGGCGTGGCATTAATCGACGGGA
ATATTATTCGCCCACAACGATTAATTACACAATCCGCATAA

Upstream 100 bases:

>100_bases
TTATTTACGATCAATCCTTAGTGGAAGGGATGCGTATGTCAAATCTTGTGACAAATGTTGTGCGTGAAGATGAGAAAAAA
CACGTTGAGGAAAGCAACTA

Downstream 100 bases:

>100_bases
CTTCATTGCCTTTCCTGTAAAATTCTAGTAATCTCCTTGTAATTAATTTTTTACAATATATGTAAGATAAACATTACAGG
TGAGTTTTATGGAAGCCCTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTA
KLGERTDTSDAEGQVVETREVHVETPQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNF
IEYHAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
AVSKLPALHLDVEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVALIDGNIIRPQRLITQSA

Sequences:

>Translated_306_residues
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTA
KLGERTDTSDAEGQVVETREVHVETPQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNF
IEYHAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
AVSKLPALHLDVEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVALIDGNIIRPQRLITQSA
>Mature_305_residues
SRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTAK
LGERTDTSDAEGQVVETREVHVETPQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFI
EYHAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDTA
VSKLPALHLDVEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVALIDGNIIRPQRLITQSA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=221, Percent_Identity=34.841628959276, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI215599015, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4503337, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=58.3333333333333, Blast_Score=356, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17553978, Length=193, Percent_Identity=32.1243523316062, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=192, Percent_Identity=31.25, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=31.7460317460317, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364187, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364185, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364183, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI62471759, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17975520, Length=192, Percent_Identity=29.6875, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34162; Mature: 34031

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYLVTAKLGERTDTSDAEGQVVETREVHVETPQILTALEQFRGDIL
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFIEYHAPFLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCH
AVSKLPALHLDVEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVALIDGNIIRPQR
HHHHCCEEEEECHHHHCCCCCCCCCCCCCHHCCCEEEEEEECCEEEEEEEECCCCCCHHH
LITQSA
HHHCCC
>Mature Secondary Structure 
SRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYLVTAKLGERTDTSDAEGQVVETREVHVETPQILTALEQFRGDIL
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFIEYHAPFLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCH
AVSKLPALHLDVEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVALIDGNIIRPQR
HHHHCCEEEEECHHHHCCCCCCCCCCCCCHHCCCEEEEEEECCEEEEEEEECCCCCCHHH
LITQSA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]