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The map label for this gene is holD

Identifier: 148825707

GI number: 148825707

Start: 655010

End: 655414

Strand: Direct

Name: holD

Synonym: CGSHiEE_03260

Alternate gene names: 148825707

Gene position: 655010-655414 (Clockwise)

Preceding gene: 148825705

Following gene: 148825708

Centisome position: 36.13

GC content: 40.99

Gene sequence:

>405_bases
ATGAACAGACGCGATCTTCTTTTACAAGAAATGGGCATTTCCCAGTGGGAATTATATCGCCCCGAGGTACTGCAAGGTTC
AGTAGGAATTAGTGTGGCAGAGAATATTCGCCTTATCACTGTTTCCGATGAAAATATCAGTAGCTCGCCTTTGTTGGCTG
ATGTGCTGTTAAGCCTTAATCTTAAAAAAGAAAATTGTTTATGTTTGAATTACGATCAAATCCAGCATATGGAATGTAAA
CAGCCTATTCGTTATTGGTTACTATCAGAAAATAGCGACCAAATTGACCGCACTTTGCCATTTTGCAAGCAGGCTGAGCA
GGTTTATCGCTCGCCAAGTTGGCAGCAATTTCAATCTAATCATCAAGCCAAACGAGCGTTGTGGCAACAAATTCAGCAGC
CTTAA

Upstream 100 bases:

>100_bases
GAATCCTGTGGGTTATTGGTTAAAATTACGCACCATTTTAGCAAAGATTAATAAAAGAAATTAACACTATTTTTCAAGCA
ACGTTTTTAAAAATTCACTT

Downstream 100 bases:

>100_bases
TTATGTCTATTATTTCTCAAATTGAAGCCTGTGATTTCGAGCGATTGTATGAAATCGAGCAGCAAGCACATCTGATGCCT
TGGTCGTTAGGGACGTTAAA

Product: DNA polymerase III subunit psi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRLITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP

Sequences:

>Translated_134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRLITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP
>Mature_134_residues
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRLITVSDENISSSPLLADVLLSLNLKKENCLCLNYDQIQHMECK
QPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSNHQAKRALWQQIQQP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown

COG id: COG3050

COG function: function code L; DNA polymerase III, psi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790831, Length=131, Percent_Identity=32.824427480916, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLD_HAEIN (P43750)

Other databases:

- EMBL:   L42023
- PIR:   E64042
- RefSeq:   NP_438184.1
- ProteinModelPortal:   P43750
- SMR:   P43750
- GeneID:   950908
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0011
- NMPDR:   fig|71421.1.peg.10
- TIGR:   HI_0011
- HOGENOM:   HBG636949
- OMA:   KPQVLKG
- ProtClustDB:   PRK06856
- BioCyc:   HINF71421:HI_0011-MONOMER
- BRENDA:   2.7.7.7
- InterPro:   IPR004615
- InterPro:   IPR018382
- Gene3D:   G3DSA:3.40.50.10220
- PIRSF:   PIRSF029225
- TIGRFAMs:   TIGR00664

Pfam domain/function: PF03603 DNA_III_psi; SSF102220 DNA_pol_III_psi

EC number: =2.7.7.7

Molecular weight: Translated: 15718; Mature: 15718

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRLITVSDENISSSPLLADVLLSLN
CCHHHHHHHHHCCCHHHHCCCHHHCCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHCC
LKKENCLCLNYDQIQHMECKQPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSN
CCCCCEEEECHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HQAKRALWQQIQQP
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNRRDLLLQEMGISQWELYRPEVLQGSVGISVAENIRLITVSDENISSSPLLADVLLSLN
CCHHHHHHHHHCCCHHHHCCCHHHCCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHCC
LKKENCLCLNYDQIQHMECKQPIRYWLLSENSDQIDRTLPFCKQAEQVYRSPSWQQFQSN
CCCCCEEEECHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HQAKRALWQQIQQP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800