Click here to switch to the map view.

The map label for this gene is dnaQ [H]

Identifier: 148825595

GI number: 148825595

Start: 512327

End: 513097

Strand: Direct

Name: dnaQ [H]

Synonym: CGSHiEE_02600

Alternate gene names: 148825595

Gene position: 512327-513097 (Clockwise)

Preceding gene: 148825592

Following gene: 148825596

Centisome position: 28.26

GC content: 36.32

Gene sequence:

>771_bases
ATGATTAATCCCAATCGCCAAATTGTATTAGATACTGAAACTACAGGTATGAATCAGCTTGGCGCGCATTATGAAGGACA
CTGCATTATTGAAATTGGTGCAGTTGAATTAATAAATCGTCGTTACACAGGTAATAATTTCCATATTTATATTAAGCCAG
ATCGCCCTGTTGATCCTGATGCGATTAAAGTTCATGGTATTACCGATGAAATGTTAGCGGATAAACCTGAATTCAAAGAC
CTAGCCCAAGATTTTTTGGATTATATTAATGGTGCGGAATTGCTTATTCACAATGCGCCCTTTGACGTTGGATTTATGGA
TTATGAATTTCGTAAACTTAACCTTAATGTGAAAACTGACGATATTTGTTTGGTGACAGATACGCTACAAATGGCGCGTC
AGATGTATCCTGGAAAACGCAATAATTTGGATGCACTTTGTGATCGTTTAGGTATTGATAATAGCAAACGTACTTTGCAC
GGTGCGTTACTGGATGCCGAGATTTTGGCGGATGTTTATTTGATGATGACGGGGGGGCAAACAAATTTATTCGATGAAGA
AGAATCTGTTGAGTCTGAAGTTATCCGTGTTGTGCAAGAAAAAACGGCGGAAGAAATAAAAAGTGCGGTAGATTTTTCGC
ACAATTTAAAATTACTTCAACCTACTAATGATGAGCTACAAGCGCATTTAGAATTATTAAAGATGCTTAATAAAAAAAGT
GGAAATAATTGTCTTTGGGATAAACGCTTTGGCAATAATAATGTGCATTAA

Upstream 100 bases:

>100_bases
AGCAAGATCCATCAGTAAAAATTTCAATCTGTTTTTGCATAACAAAAAGGTTGTTGTGTAGAATGGCAAGTATTTTAATG
GAAAGAGAGAGGTTGAACAA

Downstream 100 bases:

>100_bases
TCGTTTGGAATTTAAGCAGGTAAGTAAAATTTAATAAAAAAAGATTGACGAGATTTGTGTTCATCATTAAGATACGCAGC
ACTTAGCGGAGTGGTAGTTC

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNFHIYIKPDRPVDPDAIKVHGITDEMLADKPEFKD
LAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESEVIRVVQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLELLKMLNKKS
GNNCLWDKRFGNNNVH

Sequences:

>Translated_256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNFHIYIKPDRPVDPDAIKVHGITDEMLADKPEFKD
LAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESEVIRVVQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLELLKMLNKKS
GNNCLWDKRFGNNNVH
>Mature_256_residues
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNFHIYIKPDRPVDPDAIKVHGITDEMLADKPEFKD
LAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTDDICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLH
GALLDAEILADVYLMMTGGQTNLFDEEESVESEVIRVVQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLELLKMLNKKS
GNNCLWDKRFGNNNVH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=243, Percent_Identity=60.4938271604938, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788149, Length=181, Percent_Identity=30.3867403314917, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 29145; Mature: 29145

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNFHIYIKPDRPVDPD
CCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECHHHHHHHEECCCEEEEEECCCCCCCCC
AIKVHGITDEMLADKPEFKDLAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTD
CEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEECEEEEEEEEEECCC
DICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLHGALLDAEILADVYLMMTGGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
TNLFDEEESVESEVIRVVQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLELLKMLNKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
GNNCLWDKRFGNNNVH
CCCCEEECCCCCCCCC
>Mature Secondary Structure
MINPNRQIVLDTETTGMNQLGAHYEGHCIIEIGAVELINRRYTGNNFHIYIKPDRPVDPD
CCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECHHHHHHHEECCCEEEEEECCCCCCCCC
AIKVHGITDEMLADKPEFKDLAQDFLDYINGAELLIHNAPFDVGFMDYEFRKLNLNVKTD
CEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEECEEEEEEEEEECCC
DICLVTDTLQMARQMYPGKRNNLDALCDRLGIDNSKRTLHGALLDAEILADVYLMMTGGQ
CEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
TNLFDEEESVESEVIRVVQEKTAEEIKSAVDFSHNLKLLQPTNDELQAHLELLKMLNKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
GNNCLWDKRFGNNNVH
CCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]