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The map label for this gene is nagB [H]

Identifier: 148825591

GI number: 148825591

Start: 508189

End: 509001

Strand: Direct

Name: nagB [H]

Synonym: CGSHiEE_02580

Alternate gene names: 148825591

Gene position: 508189-509001 (Clockwise)

Preceding gene: 148825590

Following gene: 148825592

Centisome position: 28.03

GC content: 38.25

Gene sequence:

>813_bases
ATGCGTTTTATTCCGTTACAAACAGAACAACAAGTCAGTTGTTGGGCTGCACAACATATCATCAATCGTATCAACGATTT
TAAACCTACCGCCGAGCGTCCTTTTGTATTGGGGTTACCTACTGGTGGTACACCACTAAAAACTTATCAAGAACTGATTC
GACTTTATCAAGCAGGCAAAGTGAGTTTCAAACACGTAGTGACTTTCAATATGGATGAATATGTGGCTTTACCAGAGGAA
CACCCTGAAAGTTACCACAGCTTTATGTATAACAATTTTTTCAATCATATCGATATTTTACCTGAAAATATCAATATTCT
TAACGGCAATACCGATGACCATAATGCAGAATGCCATCGTTATGAAGAAAAAATTAAATCTTACGGCAAAATTCATCTGT
TTATGGGGGGGGTTGGCGTAGATGGACATATCGCATTTAATGAACCTGCTTCTTCATTAAGTTCTCGCACCCGTATTAAA
ACGCTAACGCAAGATACATTAATTGCAAATTCACGCTTCTTTAATAATGATGTTACTCAAGTGCCGAAATATGCTTTAAC
CATTGGTGTCGGTACATTATTAGATGCAGAAGAAGTGATGATTTTAGCAACAGGACATCAAAAAGCACTGGCAGTGCAAG
CTGCAGTTGAAGGCAGTATTAACCACTTATGGACAGTCAGTGCATTACAAATGCACCGTCATTTCTTGCTTGTTTGTGAT
GAAGCTGCTCAACAAGAGTTAAAAGTGAAAACCGTGAAATATTTTACTGAATTAGAAGGCGCGGTTGCTGGTACGGATTA
TCAGGGTAAATAG

Upstream 100 bases:

>100_bases
AAATTACCGCACTTATAATGATCCTTTGGTGAAGGGTGGGCTAAAGTCCAGCCTACGATATGAATAGGATCATTACGGTC
AATAATAAAGGAAGGCTAAT

Downstream 100 bases:

>100_bases
AGATCTATTCTTCATCTTTATGTAGGGGCGAATTACATTCGCCCTATAATCACAAACAATCATAGGGCAAATTGTCCTTA
CGTTTAAGAGCAATATAGTT

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRFIPLQTEQQVSCWAAQHIINRINDFKPTAERPFVLGLPTGGTPLKTYQELIRLYQAGKVSFKHVVTFNMDEYVALPEE
HPESYHSFMYNNFFNHIDILPENINILNGNTDDHNAECHRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTQDTLIANSRFFNNDVTQVPKYALTIGVGTLLDAEEVMILATGHQKALAVQAAVEGSINHLWTVSALQMHRHFLLVCD
EAAQQELKVKTVKYFTELEGAVAGTDYQGK

Sequences:

>Translated_270_residues
MRFIPLQTEQQVSCWAAQHIINRINDFKPTAERPFVLGLPTGGTPLKTYQELIRLYQAGKVSFKHVVTFNMDEYVALPEE
HPESYHSFMYNNFFNHIDILPENINILNGNTDDHNAECHRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTQDTLIANSRFFNNDVTQVPKYALTIGVGTLLDAEEVMILATGHQKALAVQAAVEGSINHLWTVSALQMHRHFLLVCD
EAAQQELKVKTVKYFTELEGAVAGTDYQGK
>Mature_270_residues
MRFIPLQTEQQVSCWAAQHIINRINDFKPTAERPFVLGLPTGGTPLKTYQELIRLYQAGKVSFKHVVTFNMDEYVALPEE
HPESYHSFMYNNFFNHIDILPENINILNGNTDDHNAECHRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
TLTQDTLIANSRFFNNDVTQVPKYALTIGVGTLLDAEEVMILATGHQKALAVQAAVEGSINHLWTVSALQMHRHFLLVCD
EAAQQELKVKTVKYFTELEGAVAGTDYQGK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=61.6279069767442, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=58.9147286821705, Blast_Score=322, Evalue=2e-88,
Organism=Escherichia coli, GI1786893, Length=260, Percent_Identity=73.8461538461539, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789530, Length=242, Percent_Identity=27.6859504132231, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=25, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=58.5271317829457, Blast_Score=312, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24581960, Length=255, Percent_Identity=60.7843137254902, Blast_Score=343, Evalue=7e-95,
Organism=Drosophila melanogaster, GI19920764, Length=255, Percent_Identity=60.7843137254902, Blast_Score=343, Evalue=7e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 30424; Mature: 30424

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIPLQTEQQVSCWAAQHIINRINDFKPTAERPFVLGLPTGGTPLKTYQELIRLYQAGK
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVALPEEHPESYHSFMYNNFFNHIDILPENINILNGNTDDHNAECHR
CEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTQDTLIANSRFFNNDVTQ
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
VPKYALTIGVGTLLDAEEVMILATGHQKALAVQAAVEGSINHLWTVSALQMHRHFLLVCD
CCCEEEEECCCHHCCCCCEEEEEECCCHHEEEHHHHCCCCHHEEHHHHHHHHHHEEEEEC
EAAQQELKVKTVKYFTELEGAVAGTDYQGK
HHHHHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure
MRFIPLQTEQQVSCWAAQHIINRINDFKPTAERPFVLGLPTGGTPLKTYQELIRLYQAGK
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVALPEEHPESYHSFMYNNFFNHIDILPENINILNGNTDDHNAECHR
CEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTQDTLIANSRFFNNDVTQ
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
VPKYALTIGVGTLLDAEEVMILATGHQKALAVQAAVEGSINHLWTVSALQMHRHFLLVCD
CCCEEEEECCCHHCCCCCEEEEEECCCHHEEEHHHHCCCCHHEEHHHHHHHHHHEEEEEC
EAAQQELKVKTVKYFTELEGAVAGTDYQGK
HHHHHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA