Click here to switch to the map view.

The map label for this gene is rapA [H]

Identifier: 148825511

GI number: 148825511

Start: 400761

End: 403532

Strand: Direct

Name: rapA [H]

Synonym: CGSHiEE_02060

Alternate gene names: 148825511

Gene position: 400761-403532 (Clockwise)

Preceding gene: 148825504

Following gene: 148825512

Centisome position: 22.1

GC content: 41.16

Gene sequence:

>2772_bases
ATGCCCTTTGCTATTGGTCAGCGTTGGTTAAGTGAAAGTGAAAACGCTTTAGGATTAGGCGTTATTACCGCATTAGATCA
ACGAACTGTTACTATTTATTTCCCCGCAGCAGATGAAACGAGGATATATGCTATAGCGCAAGCACCTTTAAGTCGAATTG
TATTTAGCAAAGGCGAAACATTATCCCATCAAGCTGGCTGGCAAGGCGAGATTTTAGACGTTCAAAATATGAACGGTTTG
TTGTTTTATTTAGTTAAAAATCCACAAGATGAAGATGTTATTGTGCAAGAACGTGATATTTCGCCGATTATTTCTTTTAG
TCAAGCGAAAGATCGTCTGTTTTCAGCGCAAATTGATCGCAGTACTCATTTTGCATTGCGTTATCGCACGCTTTGTCATC
AGCAAGCACAATTTAAATCGCCATTGCGTGGTTTACGCGGAACGCGTGCGGGTTTAATTCCCCATCAGCTTCATATTGCA
GCTGAAGTCGGTAATCGAGTTAATCCTCGCGTGTTATTGGCGGATGAGGTTGGTTTAGGGAAAACCATTGAAGCAGGAAT
GATTTTGCAGAATCAACTTTTTGCTGAGAAAGTGCAACGTGTTCTTATTATCGTGCCAGAAACCTTGCAACATCAATGGC
TGGTGGAAATGCTTCGTCGTTTTAATTTGCATTTTGCACTTTTTGATGAAGAACGTTGCAACGATTTTGATCTTGATGCC
GTTAATCCGTTTACAACTGAAAGCTTAATTATTTGTTCGCTGAATTGGTTAGAAACGCATCCAAACCGAGTAGAGCAGGC
ACTTGATGCTCAATTTGATTGCTTAATTGTGGACGAAGCACATCATCTGGTGTGGTCTGAAACCTCGCCAAGTGCAGCTT
ATTTATTCGTTGAACAGCTTGCTCGTATCATTCCATCTGTCTTGTTACTCACGGCAACCCCAGAACAACTTGGGCAAGAA
AGTCATTTTGCTCGTTTGCGCTTACTGGATCCAGAACGTTTCTTTGATTATCAAACCTTTGTGAAAGAGCAGGAACACTA
TCAACCTGTGGTTAATGCCGTTGAATCTTTGTTGGCAAATAAGGCATTAAGTGCGGTAGAAAAAAATCATATTTCTGATT
TGTTGCTTGAACAAGATGTCGAGCCACTTTTTAAAGCGATTGCATCAAATAATGATGAAGAACAGCAGCGTGCCCGTCAG
GAATTGATTCAAGCCTTAATTGATCGTCACGGCACGGGGCGAATGTTATTCCGCAATACACGTCAAGGCGTGAAAGGTTT
TCCGCACCGTGTTTATCATCAAATTACTCTCTCTGAAGAGAATGATAAAATTGATTGGCTGATTGATTTCCTCAAACTAC
ATCGCGATGAAAAAATCTTTGTGATTTGCCAGACGGCGGCAACTGCGATTCAGTTAGAACAAATTTTACGTGAACGTGAG
GCGATTCGCGCCGCAGTGTTTCACGAAAAAATGTCGATTATCGAACGGGATAGAGCCGCGGCTTATTTTGCGGATTTAGA
AAACGGCGCGCAGGTTTTACTGAGCTCGAGTATTGGCTCGGAAGGGCGTAACTTCCAATTCGCAGCAAATTTAGTGTTAT
TTGATTTACCGACAAATCCTGATTTATTGGAACAATGTATTGGCCGATTAGATCGTATTGGACAAAAACGTGATGTGCAA
ATTTATATGCCTTGTGCGAAAGATTCACCTCAAAGTCGTTTAGCACGTTGGTACAATGAGGGATTGAATGCTTTTGAACA
AACTTGTCCAATGGGAATGGCATTATTTTCTCAGTTTGCCGATGAGTTGGAAAAAGTGCGGTCAAATTCTACCGCACTTT
CAGAGAATGAATTTTCTGGATTATTAAAACAAACTAAAACCGCACGGGAAAAACTCAAGATTGAGTTAGAAAAAGGTCGC
GATCGTTTATTAGAGCTAAATTCTCATGGCGGTGAGCAAGCACAAGCGTTAGCGGATCAAATCGCTGATGAAGATAATTC
GCCAGAGCTTGTGAATTTTGCTTTAAAATTATTTGATATTATTGGCGTAGAGCAAGAAGATCTTGGGGCTAATAGTATTG
TAATTAGTCCAACTGGGACAATGCTTGTGCCCGATTTTCCAGGTTTAAAAGAAGAAGGTGTGACGGTTACCTTTGATCGA
GAACTTGCCCTTGCCCGCGAAGAAATGGAATTCTTGACTTGGGATCATCCTATGATTCGTCAAGGTATTGATCTTGTTGC
TTCGGGGGATATTGGCAAAGCCGCAATGGCATTGCTTGTGAATAAACAACTGCCCGCTGGAACCTTATTAATTGAATTGA
TTTACGTGGTGGAAAGCCAATCGCCAAAAGGGTTACAGCTCAATCGTTTCTTGCCACCAACGCCTATTCGTCTGCTGCTT
GATAACAAAGGAAATAACATGGGTGAGCAGGTTGCCTTTGAAACATTGCATAGCAAATTGAAACCACTTGGTAAAAATAT
TGCTAATCAAATGGTAAAAATGGCACGGAGCAACATTGAATCCTTGATCACGCGAGGTGATCAACTGGTAAAATCCTTGG
CAGAACCGATTATTGCTGAAGCTAAAAATCAAGCAGATCAACAATTAAGCGCAGAAATTAACCGTTTGCAGGCTTTGCGA
GCAGTGAATAAAAATATTCGCCAAAGCGAAATTGATATTTTGGAACAACAACGAACACAATCACTGGACGAGCTTTCTAA
AGCAAATTGGCGTTTGGATTGCTTGCGTGTCATTGTGACGAACAAGGAATAA

Upstream 100 bases:

>100_bases
CCTAAAAGTGCGGTCAATTTTTTGTGAGTTTCTTTTCAAGAGTATGCAAGCTACAATACGCCCCCAAGTTTTATCAATGT
AAATCAGAAAGAGAATAATT

Downstream 100 bases:

>100_bases
AAATGGCATTGATTGAATACAATCCGCCCCTTGAACCTTATTTGGATATTATTTATCAAGATAATCATTTGTGCGTGGTG
AACAAACCTAGCGGTTTGCT

Product: ATP-dependent helicase HepA

Products: NA

Alternate protein names: ATP-dependent helicase hepA [H]

Number of amino acids: Translated: 923; Mature: 922

Protein sequence:

>923_residues
MPFAIGQRWLSESENALGLGVITALDQRTVTIYFPAADETRIYAIAQAPLSRIVFSKGETLSHQAGWQGEILDVQNMNGL
LFYLVKNPQDEDVIVQERDISPIISFSQAKDRLFSAQIDRSTHFALRYRTLCHQQAQFKSPLRGLRGTRAGLIPHQLHIA
AEVGNRVNPRVLLADEVGLGKTIEAGMILQNQLFAEKVQRVLIIVPETLQHQWLVEMLRRFNLHFALFDEERCNDFDLDA
VNPFTTESLIICSLNWLETHPNRVEQALDAQFDCLIVDEAHHLVWSETSPSAAYLFVEQLARIIPSVLLLTATPEQLGQE
SHFARLRLLDPERFFDYQTFVKEQEHYQPVVNAVESLLANKALSAVEKNHISDLLLEQDVEPLFKAIASNNDEEQQRARQ
ELIQALIDRHGTGRMLFRNTRQGVKGFPHRVYHQITLSEENDKIDWLIDFLKLHRDEKIFVICQTAATAIQLEQILRERE
AIRAAVFHEKMSIIERDRAAAYFADLENGAQVLLSSSIGSEGRNFQFAANLVLFDLPTNPDLLEQCIGRLDRIGQKRDVQ
IYMPCAKDSPQSRLARWYNEGLNAFEQTCPMGMALFSQFADELEKVRSNSTALSENEFSGLLKQTKTAREKLKIELEKGR
DRLLELNSHGGEQAQALADQIADEDNSPELVNFALKLFDIIGVEQEDLGANSIVISPTGTMLVPDFPGLKEEGVTVTFDR
ELALAREEMEFLTWDHPMIRQGIDLVASGDIGKAAMALLVNKQLPAGTLLIELIYVVESQSPKGLQLNRFLPPTPIRLLL
DNKGNNMGEQVAFETLHSKLKPLGKNIANQMVKMARSNIESLITRGDQLVKSLAEPIIAEAKNQADQQLSAEINRLQALR
AVNKNIRQSEIDILEQQRTQSLDELSKANWRLDCLRVIVTNKE

Sequences:

>Translated_923_residues
MPFAIGQRWLSESENALGLGVITALDQRTVTIYFPAADETRIYAIAQAPLSRIVFSKGETLSHQAGWQGEILDVQNMNGL
LFYLVKNPQDEDVIVQERDISPIISFSQAKDRLFSAQIDRSTHFALRYRTLCHQQAQFKSPLRGLRGTRAGLIPHQLHIA
AEVGNRVNPRVLLADEVGLGKTIEAGMILQNQLFAEKVQRVLIIVPETLQHQWLVEMLRRFNLHFALFDEERCNDFDLDA
VNPFTTESLIICSLNWLETHPNRVEQALDAQFDCLIVDEAHHLVWSETSPSAAYLFVEQLARIIPSVLLLTATPEQLGQE
SHFARLRLLDPERFFDYQTFVKEQEHYQPVVNAVESLLANKALSAVEKNHISDLLLEQDVEPLFKAIASNNDEEQQRARQ
ELIQALIDRHGTGRMLFRNTRQGVKGFPHRVYHQITLSEENDKIDWLIDFLKLHRDEKIFVICQTAATAIQLEQILRERE
AIRAAVFHEKMSIIERDRAAAYFADLENGAQVLLSSSIGSEGRNFQFAANLVLFDLPTNPDLLEQCIGRLDRIGQKRDVQ
IYMPCAKDSPQSRLARWYNEGLNAFEQTCPMGMALFSQFADELEKVRSNSTALSENEFSGLLKQTKTAREKLKIELEKGR
DRLLELNSHGGEQAQALADQIADEDNSPELVNFALKLFDIIGVEQEDLGANSIVISPTGTMLVPDFPGLKEEGVTVTFDR
ELALAREEMEFLTWDHPMIRQGIDLVASGDIGKAAMALLVNKQLPAGTLLIELIYVVESQSPKGLQLNRFLPPTPIRLLL
DNKGNNMGEQVAFETLHSKLKPLGKNIANQMVKMARSNIESLITRGDQLVKSLAEPIIAEAKNQADQQLSAEINRLQALR
AVNKNIRQSEIDILEQQRTQSLDELSKANWRLDCLRVIVTNKE
>Mature_922_residues
PFAIGQRWLSESENALGLGVITALDQRTVTIYFPAADETRIYAIAQAPLSRIVFSKGETLSHQAGWQGEILDVQNMNGLL
FYLVKNPQDEDVIVQERDISPIISFSQAKDRLFSAQIDRSTHFALRYRTLCHQQAQFKSPLRGLRGTRAGLIPHQLHIAA
EVGNRVNPRVLLADEVGLGKTIEAGMILQNQLFAEKVQRVLIIVPETLQHQWLVEMLRRFNLHFALFDEERCNDFDLDAV
NPFTTESLIICSLNWLETHPNRVEQALDAQFDCLIVDEAHHLVWSETSPSAAYLFVEQLARIIPSVLLLTATPEQLGQES
HFARLRLLDPERFFDYQTFVKEQEHYQPVVNAVESLLANKALSAVEKNHISDLLLEQDVEPLFKAIASNNDEEQQRARQE
LIQALIDRHGTGRMLFRNTRQGVKGFPHRVYHQITLSEENDKIDWLIDFLKLHRDEKIFVICQTAATAIQLEQILREREA
IRAAVFHEKMSIIERDRAAAYFADLENGAQVLLSSSIGSEGRNFQFAANLVLFDLPTNPDLLEQCIGRLDRIGQKRDVQI
YMPCAKDSPQSRLARWYNEGLNAFEQTCPMGMALFSQFADELEKVRSNSTALSENEFSGLLKQTKTAREKLKIELEKGRD
RLLELNSHGGEQAQALADQIADEDNSPELVNFALKLFDIIGVEQEDLGANSIVISPTGTMLVPDFPGLKEEGVTVTFDRE
LALAREEMEFLTWDHPMIRQGIDLVASGDIGKAAMALLVNKQLPAGTLLIELIYVVESQSPKGLQLNRFLPPTPIRLLLD
NKGNNMGEQVAFETLHSKLKPLGKNIANQMVKMARSNIESLITRGDQLVKSLAEPIIAEAKNQADQQLSAEINRLQALRA
VNKNIRQSEIDILEQQRTQSLDELSKANWRLDCLRVIVTNKE

Specific function: Transcription regulator that activates transcription by stimulating RNA polymerase (RNAP) recycling in case of stress conditions such as supercoiled DNA or high salt concentrations. Probably acts by releasing the RNAP, when it is trapped or immobilized on

COG id: COG0553

COG function: function code KL; Superfamily II DNA/RNA helicases, SNF2 family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1786245, Length=971, Percent_Identity=52.6261585993821, Blast_Score=1041, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR022737
- InterPro:   IPR000330 [H]

Pfam domain/function: PF00271 Helicase_C; PF12137 RapA_C; PF00176 SNF2_N [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 104356; Mature: 104224

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFAIGQRWLSESENALGLGVITALDQRTVTIYFPAADETRIYAIAQAPLSRIVFSKGET
CCCCHHHHHHCCCCCCCEEHHHEECCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCC
LSHQAGWQGEILDVQNMNGLLFYLVKNPQDEDVIVQERDISPIISFSQAKDRLFSAQIDR
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
STHFALRYRTLCHQQAQFKSPLRGLRGTRAGLIPHQLHIAAEVGNRVNPRVLLADEVGLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEHHHHCCCCCCEEEEEECCCCC
KTIEAGMILQNQLFAEKVQRVLIIVPETLQHQWLVEMLRRFNLHFALFDEERCNDFDLDA
CCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
VNPFTTESLIICSLNWLETHPNRVEQALDAQFDCLIVDEAHHLVWSETSPSAAYLFVEQL
CCCCCCCCEEEEECHHHHCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHHHHHHHH
ARIIPSVLLLTATPEQLGQESHFARLRLLDPERFFDYQTFVKEQEHYQPVVNAVESLLAN
HHHHHHHHEEECCHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALSAVEKNHISDLLLEQDVEPLFKAIASNNDEEQQRARQELIQALIDRHGTGRMLFRNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RQGVKGFPHRVYHQITLSEENDKIDWLIDFLKLHRDEKIFVICQTAATAIQLEQILRERE
HHHCCCCCHHHEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
AIRAAVFHEKMSIIERDRAAAYFADLENGAQVLLSSSIGSEGRNFQFAANLVLFDLPTNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCEEEEEEEEEEECCCCH
DLLEQCIGRLDRIGQKRDVQIYMPCAKDSPQSRLARWYNEGLNAFEQTCPMGMALFSQFA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DELEKVRSNSTALSENEFSGLLKQTKTAREKLKIELEKGRDRLLELNSHGGEQAQALADQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEHHHCCCCCEEEECCCCCHHHHHHHHH
IADEDNSPELVNFALKLFDIIGVEQEDLGANSIVISPTGTMLVPDFPGLKEEGVTVTFDR
HCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEEECCCCCEECCCCCCCCCCCEEEEECH
ELALAREEMEFLTWDHPMIRQGIDLVASGDIGKAAMALLVNKQLPAGTLLIELIYVVESQ
HHHHHHHHHHHHCCCCHHHHCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPKGLQLNRFLPPTPIRLLLDNKGNNMGEQVAFETLHSKLKPLGKNIANQMVKMARSNIE
CCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLITRGDQLVKSLAEPIIAEAKNQADQQLSAEINRLQALRAVNKNIRQSEIDILEQQRTQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLDELSKANWRLDCLRVIVTNKE
HHHHHHHCCCHHEEEEEEEECCC
>Mature Secondary Structure 
PFAIGQRWLSESENALGLGVITALDQRTVTIYFPAADETRIYAIAQAPLSRIVFSKGET
CCCHHHHHHCCCCCCCEEHHHEECCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCCCCC
LSHQAGWQGEILDVQNMNGLLFYLVKNPQDEDVIVQERDISPIISFSQAKDRLFSAQIDR
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
STHFALRYRTLCHQQAQFKSPLRGLRGTRAGLIPHQLHIAAEVGNRVNPRVLLADEVGLG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEHHHHCCCCCCEEEEEECCCCC
KTIEAGMILQNQLFAEKVQRVLIIVPETLQHQWLVEMLRRFNLHFALFDEERCNDFDLDA
CCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
VNPFTTESLIICSLNWLETHPNRVEQALDAQFDCLIVDEAHHLVWSETSPSAAYLFVEQL
CCCCCCCCEEEEECHHHHCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHHHHHHHH
ARIIPSVLLLTATPEQLGQESHFARLRLLDPERFFDYQTFVKEQEHYQPVVNAVESLLAN
HHHHHHHHEEECCHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALSAVEKNHISDLLLEQDVEPLFKAIASNNDEEQQRARQELIQALIDRHGTGRMLFRNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RQGVKGFPHRVYHQITLSEENDKIDWLIDFLKLHRDEKIFVICQTAATAIQLEQILRERE
HHHCCCCCHHHEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
AIRAAVFHEKMSIIERDRAAAYFADLENGAQVLLSSSIGSEGRNFQFAANLVLFDLPTNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCEEEEEEEEEEECCCCH
DLLEQCIGRLDRIGQKRDVQIYMPCAKDSPQSRLARWYNEGLNAFEQTCPMGMALFSQFA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DELEKVRSNSTALSENEFSGLLKQTKTAREKLKIELEKGRDRLLELNSHGGEQAQALADQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEHHHCCCCCEEEECCCCCHHHHHHHHH
IADEDNSPELVNFALKLFDIIGVEQEDLGANSIVISPTGTMLVPDFPGLKEEGVTVTFDR
HCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEEECCCCCEECCCCCCCCCCCEEEEECH
ELALAREEMEFLTWDHPMIRQGIDLVASGDIGKAAMALLVNKQLPAGTLLIELIYVVESQ
HHHHHHHHHHHHCCCCHHHHCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPKGLQLNRFLPPTPIRLLLDNKGNNMGEQVAFETLHSKLKPLGKNIANQMVKMARSNIE
CCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLITRGDQLVKSLAEPIIAEAKNQADQQLSAEINRLQALRAVNKNIRQSEIDILEQQRTQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLDELSKANWRLDCLRVIVTNKE
HHHHHHHCCCHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA