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The map label for this gene is rph [H]

Identifier: 148825447

GI number: 148825447

Start: 317143

End: 317859

Strand: Direct

Name: rph [H]

Synonym: CGSHiEE_01685

Alternate gene names: 148825447

Gene position: 317143-317859 (Clockwise)

Preceding gene: 148825446

Following gene: 148825448

Centisome position: 17.49

GC content: 44.35

Gene sequence:

>717_bases
ATGCGTCCAAATAATCGAGAAAATAATCAACCCCGCCAAATTAAAATTACCCGTAACTACACCAAGCATGCTGAAGGTTC
TGTGCTTGTTGAATTTGGGGACACCAAAGTGCTTTGTACTGCAACAGTGGAAGATGCTGTACCACGTTTCTTAAAAGGAC
AAGGGCAAGGTTGGGTAACGGCTGAATATGGAATGTTGCCACGTTCAACACATAGTCGTATGCAACGTGAGGCGGCTAAA
GGTAAACAAGGCGGACGCACAATGGAAATTCAACGCTTAATTGCCCGCTCTTTGCGCGCCATGGTGGATCTTAAAGCACT
GGGTGAACGTGCGATTACTTTAGATTGCGATGTTATTCAAGCGGATGGCGGCACACGAACTGCATCCATTACTGGTGCTG
CAGTCGCATTATGTGATGCGATCAACAGTTTAATTGAGAATGGCACGTTAAAAACCAACCCCATTAAAGGGCTAGTTTCG
GCGATTTCTGTAGGGATTGTTGATGGGCAAGCTGTTTGTGATTTGGAATACGTGGAAGATTCTGCAGCGGAAACCGATAT
GAATGTTGTGATGATGGAAGATGGTCGCATGATTGAGGTGCAAGGCACTGCAGAAGGCGAGCCATTTAGCCATGAAGAAT
TATTAACATTATTAGATTTAGCGAAACAAGGCTGTAATCAAATTTTCATTGCTCAACGTGAAGCGCTAGGCTTATAA

Upstream 100 bases:

>100_bases
AATCAATATCTTAGCGAATTTTAACAAAAATAAAAACTAAAGCGTGCTAGAATAGTGCGCTTTTTTATTACATTGTTCGA
AACCATATCAAGGAATAATT

Downstream 100 bases:

>100_bases
ACACAGTTTATAGGAGTTAAAAAATGGAACAATATAAACGCGATTTTATCGAATTTGCTTTAAGCCGAAATGTATTGAAA
TTCGGCGAGTTTACTTTGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINSLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL

Sequences:

>Translated_238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINSLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
>Mature_238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINSLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=82.4644549763033, Blast_Score=369, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71981632, Length=238, Percent_Identity=25.2100840336134, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25820; Mature: 25820

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAAVALCDAINSLIENGTLKTNPIKGLVSAISVGIVDGQAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCEEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCC
>Mature Secondary Structure
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAAVALCDAINSLIENGTLKTNPIKGLVSAISVGIVDGQAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCEEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA