Click here to switch to the map view.

The map label for this gene is pyrE [H]

Identifier: 148825448

GI number: 148825448

Start: 317883

End: 318524

Strand: Direct

Name: pyrE [H]

Synonym: CGSHiEE_01690

Alternate gene names: 148825448

Gene position: 317883-318524 (Clockwise)

Preceding gene: 148825447

Following gene: 148825449

Centisome position: 17.53

GC content: 41.28

Gene sequence:

>642_bases
ATGGAACAATATAAACGCGATTTTATCGAATTTGCTTTAAGCCGAAATGTATTGAAATTCGGCGAGTTTACTTTGAAATC
GGGGCGTAAAAGTCCGTATTTCTTCAATGCGGGTTTGTTCAATACGGGGGCAGATTTAGCACGTTTAGGCGAGTTTTATG
CTGCGGCAATTCAAGCAAGTGCGGTAGATTTTGATGTGGTTTTTGGCCCTGCTTACAAAGGTATTCCAATTGGCACTTCA
GTTTCGGTGGCGTTATTTAATCGTTATGGTATTGATAAACCCGTGTGTTTTAATCGTAAAGAAGTGAAAGATCACGGAGA
GGGCGGCAATTTAATTGGTAGCCCATTGCAAGGAAAAATTTTGCTTGTGGATGATGTGATTACGGCTGGCACTGCAATTC
GTGAATCTATGGAATTAATCAGTGCAAATCAGGCAGAGCTTGCGGCGGTATTGATCGCTCTAAACCGTAAGGAACGAGGA
AAAGGCGAGCTTTCAGCAATTCAAGAAGTAGAACGTGATTATCAATGCCAAGTGCTATCAATCATTGATTTAGATGATTT
GATGCAATTTATCGAACAAGATCCTCGATACAGTAGCCATTTGCCAGAAATGCGTGCTTATCGTGCCGAATTTGGCGTAT
AA

Upstream 100 bases:

>100_bases
TATTAACATTATTAGATTTAGCGAAACAAGGCTGTAATCAAATTTTCATTGCTCAACGTGAAGCGCTAGGCTTATAAACA
CAGTTTATAGGAGTTAAAAA

Downstream 100 bases:

>100_bases
AACTTGAGAAATAAATTTTTGTCATCATTATAAATAACATTATAAAAATAACCGCACTTTGGCTTAACAGGGGAAAGAAT
GGGATTTATTGGAAAAATTA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANQAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV

Sequences:

>Translated_213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANQAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
>Mature_213_residues
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQASAVDFDVVFGPAYKGIPIGTS
VSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKILLVDDVITAGTAIRESMELISANQAELAAVLIALNRKERG
KGELSAIQEVERDYQCQVLSIIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=26.1111111111111, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=69.0140845070423, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17508631, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554964, Length=190, Percent_Identity=27.3684210526316, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=200, Percent_Identity=49, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323927, Length=225, Percent_Identity=44.4444444444444, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23643; Mature: 23643

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AVDFDVVFGPAYKGIPIGTSVSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKI
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMELISANQAELAAVLIALNRKERGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEE
IIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
EECHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEQYKRDFIEFALSRNVLKFGEFTLKSGRKSPYFFNAGLFNTGADLARLGEFYAAAIQAS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AVDFDVVFGPAYKGIPIGTSVSVALFNRYGIDKPVCFNRKEVKDHGEGGNLIGSPLQGKI
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCE
LLVDDVITAGTAIRESMELISANQAELAAVLIALNRKERGKGELSAIQEVERDYQCQVLS
EEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEE
IIDLDDLMQFIEQDPRYSSHLPEMRAYRAEFGV
EECHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA