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The map label for this gene is hofB [H]

Identifier: 148825423

GI number: 148825423

Start: 290307

End: 291704

Strand: Direct

Name: hofB [H]

Synonym: CGSHiEE_01565

Alternate gene names: 148825423

Gene position: 290307-291704 (Clockwise)

Preceding gene: 148825422

Following gene: 148825424

Centisome position: 16.01

GC content: 38.77

Gene sequence:

>1398_bases
ATGACGAGTTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGAGGAAGAGCAAGTTAAATATTA
CCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTG
CCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTT
TTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATAT
TAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAA
CCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTG
GGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCCAC
AGGAAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACACCTGATAAACATATTATGACTGCTGAGG
ATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTG
CGCACTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACG
TGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGTTTACAACAAC
TCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAG
TGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTG
GCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATG
AGAAAGAAATTGAACGTGTGTTAGGTAAAAACTTATGA

Upstream 100 bases:

>100_bases
AAGCCAGTGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAACGGATGCCTCTTTATTTCCAGCAAAT
TTTTGCGGAAGTGTCACACA

Downstream 100 bases:

>100_bases
CTAAAAAACTCTTTTATTATCAAGGTAGTAACGCATTAAATCAGAAACAAAAAGGCTCAATTATTGCGGATACGAAACAA
CAAGCACACTTTCAGTTAAT

Product: putative type IV pilin secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Sequences:

>Translated_465_residues
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGV
LQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSK
CGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL
>Mature_464_residues
TSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPILL
ESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=475, Percent_Identity=40.2105263157895, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1789723, Length=391, Percent_Identity=42.1994884910486, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082188, Length=349, Percent_Identity=31.2320916905444, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 53092; Mature: 52961

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLS
CCCEEEEEEHEEECCCCCEEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLSACETISFITGKPVEPILLESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDD
CCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCC
EPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQPQPLISKIFANRIISRLK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
HHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHC
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIEL
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEECCCCCEEEE
DGIIQSQINPQIGLDFSRLLRTFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLH
CCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEC
TNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKCGGNLINSCDCHQGYRGRIG
CHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLS
CCEEEEEEHEEECCCCCEEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
NLSACETISFITGKPVEPILLESSQLKELLQQLTPHQMQEEEQVKYYQHQEQSHLEQQDD
CCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCC
EPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQPQPLISKIFANRIISRLK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAEL
HHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEHHHC
GMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIEL
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEECCCCCEEEE
DGIIQSQINPQIGLDFSRLLRTFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLH
CCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEC
TNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKCGGNLINSCDCHQGYRGRIG
CHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]