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The map label for this gene is comE [H]

Identifier: 148825285

GI number: 148825285

Start: 156102

End: 157439

Strand: Direct

Name: comE [H]

Synonym: CGSHiEE_00815

Alternate gene names: 148825285

Gene position: 156102-157439 (Clockwise)

Preceding gene: 148825284

Following gene: 148825286

Centisome position: 8.61

GC content: 37.89

Gene sequence:

>1338_bases
ATGAAGAAATATTTTTTAAAGTGCGGTTATTTTTTAGTGTGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAATGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACACTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATGGGTGAGAGGTTAGAAGGCAATATTTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTAAAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGGTTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAAGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTCAGCCACACGGTAAAACTTCATTTTGCTAAAGCCTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGATCTTTGCTTTCTTCTGCGGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTTTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCGAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGTCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGGACAGAAATTCCTTACATCGTGAGTAATACTCGTAACGATACGCAATCTGTGGAATTTCGTGAGGCAGTA
CTTGGTTTGGAAGTGACGCCACATATTTCTAAAGATAACAATATCTTACTTGATTTATTGGTAAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAGCAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTATTTCACGACACAATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTCGTGATTTTCGTCACGCCGCATAT
TTTAAAAGCAGGAGAAACGTTAGAGGCGTTGAAACAAAAAAGTGCGGGGAAAAAATAA

Upstream 100 bases:

>100_bases
TGTAGAAAAAATCAACCAAAACAATGTTCAATTTATGCGTAAGCTCGGAGAGCAATGTGATAGTAGTGAATGGAAAAAAT
TAAGTTTTTAAAGGAAGATT

Downstream 100 bases:

>100_bases
CTTTTTTAGACGATGAATTTTTTTAATTTTCGCTGTATCCACTGTCGTGGCAATCTTCATATTGCAAAAAATGGGCTATG
TTCAGGTTGCCAAAAACAAA

Product: competence protein E

Products: NA

Alternate protein names: DNA transformation protein ComE [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGERLEGNISLKLNNIDMPRLLK
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSAGKK

Sequences:

>Translated_445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGERLEGNISLKLNNIDMPRLLK
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSAGKK
>Mature_445_residues
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGERLEGNISLKLNNIDMPRLLK
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSAGKK

Specific function: Involved in transformation (competence for DNA uptake) [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=436, Percent_Identity=36.9266055045872, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI87082242, Length=337, Percent_Identity=28.1899109792285, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR004846
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 49138; Mature: 49138

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGE
CCCEEHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEECC
RLEGNISLKLNNIDMPRLLKIIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHL
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSNLLVIQDEPRFVQNIKKLIAE
EEEEEEEEEHHHHHHHHHHCCCCCHHHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHH
MDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
HCCCHHHEEEEEEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCEE
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEI
EEEECCCCCCEEEEEEEECCEEEEEEHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCC
PYIVSNTRNDTQSVEFREAVLGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSI
CEEEECCCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEE
DKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDIPVIKRLFSKESERHQKRELV
EHHHCCEEEEECCCCEEEECCEEHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCEE
IFVTPHILKAGETLEALKQKSAGKK
EEECCHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGE
CCCEEHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEECC
RLEGNISLKLNNIDMPRLLKIIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHL
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSNLLVIQDEPRFVQNIKKLIAE
EEEEEEEEEHHHHHHHHHHCCCCCHHHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHH
MDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
HCCCHHHEEEEEEEEEECHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCEE
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEI
EEEECCCCCCEEEEEEEECCEEEEEEHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCC
PYIVSNTRNDTQSVEFREAVLGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSI
CEEEECCCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEE
DKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDIPVIKRLFSKESERHQKRELV
EHHHCCEEEEECCCCEEEECCEEHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCEE
IFVTPHILKAGETLEALKQKSAGKK
EEECCHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1916268; 7542800; 1856167 [H]