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The map label for this gene is fruK [H]

Identifier: 148825273

GI number: 148825273

Start: 143525

End: 144466

Strand: Direct

Name: fruK [H]

Synonym: CGSHiEE_00755

Alternate gene names: 148825273

Gene position: 143525-144466 (Clockwise)

Preceding gene: 148825272

Following gene: 148825274

Centisome position: 7.92

GC content: 40.34

Gene sequence:

>942_bases
ATGGCAAGCGTCGTAACAATTACCTTAAATGCCGCTTATGATTTAGTCGGGCGTTTAAATCGTATTCAACTTGGCGAAGT
AAATACCGTAGAAACGCTGGGCTTATTTCCTGCAGGGAAAGGCATTAATGTTGCAAAAGTACTAAAAGATTTAGGCGTTG
ATGTTGCAGTCGGTGGTTTCTTGGGCGAGGATAATTCAGCTGATTTCGAGCAAATGTTTAATCAGCACGGTTTGGAAGAT
AAATTCCACCGAGTTGATGGCAAAACACGTATCAATGTAAAAATTACCGAGACTGAAGCGGATGTGACAGATTTAAATTT
CTTGGGATATCAAATAAGCCCACAAGTTTGGCAACAATTTGTGACGGATTCTTTGGCTTATTGTTTAAATTATGACATTG
TCGCAGTTTGTGGCAGTTTACCTCGAGGTGTGTCTCCTGAATTGTTTGCTGATTGGTTAAATCAGCTTCATAAAGCTGGT
GTAAAAGTTGTGCTTGATAGTAGCAATGCTGCACTCGCCGCTGGATTAAAAGCGAAACCTTGGCTAGTAAAACCCAACCA
TCGAGAACTTGAGGCTTTGGTTGGTCATTCATTAAATAGTCTTGAAGAAATTATTGCCGCTGCACAGCAACTCAAAGCGG
AAGACATTGAAAACGTGATTATTTCAATGGGCGAAAAAGGTTCTTTATGGATTAATAATGAAGGCTCGCTCAAAGCCGAA
CCACCAAAATGTGAAAATGTAGTGAGTACCGTTGGGGCAGGGGATTCCATGGTAGCAGGCTTAATCTATGGTTTTGAAAA
AGGTTTATCGAAAGCTGAAACTTTAGCTTTTGCTACGGCTGTGTCCGCTTTTGCTGTGTCTCAAAGTAATGTTGGTGTGA
GTGATTTGAGTTTACTTGACCCCATTTTAGAAAAAGTACAAATAACGATGATTGAAGGATAG

Upstream 100 bases:

>100_bases
GTGAAAGGGACTCGTTTACGTTTTGTTGCGACGGGCGATGAAGCGCAACAGGCTATTGACGGAATCTGTGCTGTGATCGA
ATCGGGGTTAGGGGAATAGT

Downstream 100 bases:

>100_bases
TATGAAGTTATTTTTAACCCAATCAGCTAATGTAGGCAATGCGAAAGCCTATTTATTACATGAAGTTTTTCGAGCGGCTG
CGCAAAAAGCAAATGTTTCT

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVDVAVGGFLGEDNSADFEQMFNQHGLED
KFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHKAG
VKVVLDSSNAALAAGLKAKPWLVKPNHRELEALVGHSLNSLEEIIAAAQQLKAEDIENVIISMGEKGSLWINNEGSLKAE
PPKCENVVSTVGAGDSMVAGLIYGFEKGLSKAETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG

Sequences:

>Translated_313_residues
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVDVAVGGFLGEDNSADFEQMFNQHGLED
KFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHKAG
VKVVLDSSNAALAAGLKAKPWLVKPNHRELEALVGHSLNSLEEIIAAAQQLKAEDIENVIISMGEKGSLWINNEGSLKAE
PPKCENVVSTVGAGDSMVAGLIYGFEKGLSKAETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG
>Mature_312_residues
ASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVDVAVGGFLGEDNSADFEQMFNQHGLEDK
FHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQFVTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHKAGV
KVVLDSSNAALAAGLKAKPWLVKPNHRELEALVGHSLNSLEEIIAAAQQLKAEDIENVIISMGEKGSLWINNEGSLKAEP
PKCENVVSTVGAGDSMVAGLIYGFEKGLSKAETLAFATAVSAFAVSQSNVGVSDLSLLDPILEKVQITMIEG

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788493, Length=305, Percent_Identity=54.7540983606557, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788017, Length=257, Percent_Identity=29.1828793774319, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788491, Length=120, Percent_Identity=31.6666666666667, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33512; Mature: 33380

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVDVAVGGF
CCEEEEEEECHHHHHHHCCCCEEECCCCCCHHHCCCCCCCCCCHHHHHHHCCCCEEECCC
LGEDNSADFEQMFNQHGLEDKFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQF
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCHHHHCCEECCHHHHHHH
VTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHKAGVKVVLDSSNAALAAGLKAKP
HHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCC
WLVKPNHRELEALVGHSLNSLEEIIAAAQQLKAEDIENVIISMGEKGSLWINNEGSLKAE
CEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PPKCENVVSTVGAGDSMVAGLIYGFEKGLSKAETLAFATAVSAFAVSQSNVGVSDLSLLD
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
PILEKVQITMIEG
HHHHHEEEEEECC
>Mature Secondary Structure 
ASVVTITLNAAYDLVGRLNRIQLGEVNTVETLGLFPAGKGINVAKVLKDLGVDVAVGGF
CEEEEEEECHHHHHHHCCCCEEECCCCCCHHHCCCCCCCCCCHHHHHHHCCCCEEECCC
LGEDNSADFEQMFNQHGLEDKFHRVDGKTRINVKITETEADVTDLNFLGYQISPQVWQQF
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCHHHHCCEECCHHHHHHH
VTDSLAYCLNYDIVAVCGSLPRGVSPELFADWLNQLHKAGVKVVLDSSNAALAAGLKAKP
HHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCC
WLVKPNHRELEALVGHSLNSLEEIIAAAQQLKAEDIENVIISMGEKGSLWINNEGSLKAE
CEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
PPKCENVVSTVGAGDSMVAGLIYGFEKGLSKAETLAFATAVSAFAVSQSNVGVSDLSLLD
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
PILEKVQITMIEG
HHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]