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The map label for this gene is fruB [H]

Identifier: 148825272

GI number: 148825272

Start: 142024

End: 143523

Strand: Direct

Name: fruB [H]

Synonym: CGSHiEE_00750

Alternate gene names: 148825272

Gene position: 142024-143523 (Clockwise)

Preceding gene: 148825271

Following gene: 148825273

Centisome position: 7.83

GC content: 41.93

Gene sequence:

>1500_bases
ATGTTAGAACTTTCGGAAAGCAACATTCATTTAAATGCTAATGCAATTGATAAACAACAAGCGATTGAAATGGCAGCGTC
CGCATTGGTTCAAGCAGACAATGTTGAAAATGGTTACTTGCAAGGAATGTTAGCGCGCGAGCTGCAAACCTCGACCTTTT
TAGGCAATGGCATCGCTATTCCTCACGGCACCTTGGATACTCGTTTGATGGTAAAGAAAACGGGCGTGCAAGTGTTTCAA
TTTCCTCAAGGCATAGAATGGGGCGAAGGAAATATTGCTTATGTCGTGATTGGTATTGCGGCACGCTCTGATGAACATTT
GTCCTTATTGCGCCAACTAACCCATGTATTGAGCGATGAAGATACCGCAGCAAAATTAGCAAAAATAACTGATGTGGCTG
AATTTTGTGCGATTTTAATGGGCGAGACAATTGAGCCATTTGAAATTCCTGCTGCCAATATCAGTTTAGATGTAAATACC
CAAAGTTTATTAACTTTAGTTGCGATTAATGCGGGGCAATTACAGGTGCAAAGTGCGGTAGAAAATCGCTTTATTTCTGA
AGTGATAAATAATGCGGCATTACCGCTTGGCAAAGGGTTATGGGTAACGGACTCTGTCGTAGGAAATGTGAAAAATGCCT
TAGCATTTAGTCGTGCTAAAACAATTTTTAGCCACAATGGCAAGGCCGTAAAAGGCGTGATAACCGTTTCAGCCGTCGGT
GATCAAATTAATCCAACTTTGGCGCGTTTATTAGATGACGATGTTCAAACAACCTTGCTCAATGGTAATTCAACAGAAAT
TTTGACCGCACTTTTGGGTTCATCGAGTGATGTTGAAACTCAATCTGTAGAAGGTGCTGTTGTAGGAACCTTTACGATTC
GTAATGAACACGGTTTACATGCTCGCCCAAGTGCAAATTTGGTTAATGAAGTGAAAAAATTCACTTCTAAAATAACCATG
CAAAATCTTACTCGTGAAAGTGAAGTCGTCAGCGCAAAAAGTTTGATGAAGATTGTTGCGCTTGGTGTAACACAAGGTCA
TCGTTTACGTTTTGTGGCAGAGGGAGAAGATGCCAAACAAGCGATAGAATCATTGGGCAAAGCGATAGCCAATGGCTTGG
GAGAGAATGTTTCGGCAGTGCCACCGTCTGAACCCGATACTATTGAGATTATGGGCGATCAAATTCACACATCTGCGGTA
ACAGAAGATGATAATTTGCCAGCAAATGCCATTGAAGCCGTATTTGTGATTAAAAATGAGCAAGGTTTGCACGCTCGCCC
AAGTGCGATATTGGTAAACGAAGTGAAGAAATATAATGCTTCCGTAGCAGTTCAAAATCTTGATCGTAATTCTCAATTAG
TCAGTGCTAAAAGCTTGATGAAAATTGTGGCATTAGGCGTAGTGAAAGGGACTCGTTTACGTTTTGTTGCGACGGGCGAT
GAAGCGCAACAGGCTATTGACGGAATCTGTGCTGTGATCGAATCGGGGTTAGGGGAATAG

Upstream 100 bases:

>100_bases
CGCTGCTGAAAGCCTGTACATCTAAATAATTGTTTTTCAATAAGCTGCTGGTCGATTTGTTTCATCCGCAGCTTTCTTTT
TAAATTATTAAGGAGCAAGA

Downstream 100 bases:

>100_bases
TATGGCAAGCGTCGTAACAATTACCTTAAATGCCGCTTATGATTTAGTCGGGCGTTTAAATCGTATTCAACTTGGCGAAG
TAAATACCGTAGAAACGCTG

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Fructose PTS diphosphoryl transfer protein; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIIA-Fru; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLELSESNIHLNANAIDKQQAIEMAASALVQADNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIEPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLARLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTSAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGD
EAQQAIDGICAVIESGLGE

Sequences:

>Translated_499_residues
MLELSESNIHLNANAIDKQQAIEMAASALVQADNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIEPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLARLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTSAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGD
EAQQAIDGICAVIESGLGE
>Mature_499_residues
MLELSESNIHLNANAIDKQQAIEMAASALVQADNVENGYLQGMLARELQTSTFLGNGIAIPHGTLDTRLMVKKTGVQVFQ
FPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDEDTAAKLAKITDVAEFCAILMGETIEPFEIPAANISLDVNT
QSLLTLVAINAGQLQVQSAVENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
DQINPTLARLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLHARPSANLVNEVKKFTSKITM
QNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQAIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTSAV
TEDDNLPANAIEAVFVIKNEQGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGD
EAQQAIDGICAVIESGLGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=383, Percent_Identity=49.8694516971279, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=43.0555555555556, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788755, Length=79, Percent_Identity=48.1012658227848, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016258
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 53046; Mature: 53046

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELSESNIHLNANAIDKQQAIEMAASALVQADNVENGYLQGMLARELQTSTFLGNGIAI
CCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEC
PHGTLDTRLMVKKTGVQVFQFPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDE
CCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
DTAAKLAKITDVAEFCAILMGETIEPFEIPAANISLDVNTQSLLTLVAINAGQLQVQSAV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEEEEEECCCCCHHHHHH
ENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
DQINPTLARLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLH
CCCCHHHHHHHCCCHHEEEECCCCHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCC
ARPSANLVNEVKKFTSKITMQNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
AIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTSAVTEDDNLPANAIEAVFVIKNE
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCEEEEEEEECC
QGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGD
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCH
EAQQAIDGICAVIESGLGE
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLELSESNIHLNANAIDKQQAIEMAASALVQADNVENGYLQGMLARELQTSTFLGNGIAI
CCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEC
PHGTLDTRLMVKKTGVQVFQFPQGIEWGEGNIAYVVIGIAARSDEHLSLLRQLTHVLSDE
CCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
DTAAKLAKITDVAEFCAILMGETIEPFEIPAANISLDVNTQSLLTLVAINAGQLQVQSAV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEEEEEECCCCCHHHHHH
ENRFISEVINNAALPLGKGLWVTDSVVGNVKNALAFSRAKTIFSHNGKAVKGVITVSAVG
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
DQINPTLARLLDDDVQTTLLNGNSTEILTALLGSSSDVETQSVEGAVVGTFTIRNEHGLH
CCCCHHHHHHHCCCHHEEEECCCCHHHHHHHHCCCCCCCHHCCCCEEEEEEEEECCCCCC
ARPSANLVNEVKKFTSKITMQNLTRESEVVSAKSLMKIVALGVTQGHRLRFVAEGEDAKQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
AIESLGKAIANGLGENVSAVPPSEPDTIEIMGDQIHTSAVTEDDNLPANAIEAVFVIKNE
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCEEEEEEEECC
QGLHARPSAILVNEVKKYNASVAVQNLDRNSQLVSAKSLMKIVALGVVKGTRLRFVATGD
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCH
EAQQAIDGICAVIESGLGE
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 8763608 [H]