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The map label for this gene is pgk [H]

Identifier: 148825201

GI number: 148825201

Start: 67776

End: 68936

Strand: Direct

Name: pgk [H]

Synonym: CGSHiEE_00365

Alternate gene names: 148825201

Gene position: 67776-68936 (Clockwise)

Preceding gene: 148825200

Following gene: 148825202

Centisome position: 3.74

GC content: 40.05

Gene sequence:

>1161_bases
ATGTCAGTAATCAAAATGACCGATTTAGATTTAGCGGGTAAACGTGTATTTATCCGTGCAGATCTTAATGTGCCAGTAAA
AGATGGCAAAGTGACCTCAGATGCGCGTATTCGTGCGACCATTCCTACTTTAAAATTAGCCTTAGAAAAAGGTGCGAAAG
TGATGGTAACTTCTCACTTAGGTCGTCCAACTGAAGGAGAATTCAAACCAGAAGATTCTTTACAACCAGTTGTTGATTAC
TTAAAAAACGCAGGCTTTAATGTGCGTTTAGAACAAGATTATTTAAATGGTGTGGATGTTAAAGATGGCGAAATCGTTGT
TTTAGAAAACGTACGCGTAAACAAAGGCGAAAAGAAAAATGATCCAGAATTAGGTAAAAAATATGCCGCACTTTGTGATG
TATTCGTGATGGATGCATTTGGTACAGCGCACCGCGCGCAAGCATCAACTTATGGCGTTGCAGAATTTGCGCCAATCGCT
TGTGCAGGCCCATTATTAGCGGCTGAGTTAGATGCATTAGGTAAAGCATTAAAAGAACCTGCTCGTCCAATGGTGGCTAT
TGTTGGTGGTTCTAAAGTTTCCACTAAATTAGAAGTATTAAATTCTCTTTCAAAAATCGCTGACCAAATTATTGTGGGCG
GTGGCATTGCGAATACTTTCATCGCGGCAGCTGGTCACAATGTAGGTAAATCTTTATATGAAGCTGATTTAATTCCAGTT
GCGAAAGAATTAGCCGCAAATACAGATATTCCAGTTCCAGTTGATGTGCGTGTAGGTTTAGAGTTCTCTGAAACGGCAGC
AGCAACAGAAAAAGCGGTAAATGAAGTGAAAGATGATGAATCTATTTTTGATATTGGCGATAAATCAGCAGAACAATTGG
CTGAAATTATCAAAAATGCAAAAACCGTTTTGTGGAATGGCCCAGTTGGCGTGTTTGAGTTCCCTCACTTCCGTAAAGGG
ACTGAAATCATTTCTCACGCCATTGCAAACAGCGATGCATTCTCTATTGCTGGCGGCGGCGATACCTTAGCGGCTATCAA
TTTATTTGGTATTGCTGATAAGATTTCTTACATTTCAACGGGTGGTGGTGCATTCTTAGAGTTTGTAGAAGGCAAAGTAT
TACCTGCAGTAGAAATTCTTGAAAAACGTGCGAAAAACTAA

Upstream 100 bases:

>100_bases
AATAAGAATTTCTTTGATCTAAATCGTTACAACCTAGCACGAAGTAGGTATAATACGAGAAGTTTTCAATTTTAATTTTA
ACTTAACAAGAGGATAGATT

Downstream 100 bases:

>100_bases
CCGCACTTTTCCTCTATAACATATTGATGGTGGCATCAAAAATGCCATCATCAATACAAATTCTTCAAATTGTGGGCTTA
TCCCGCTAAACATAAAAAGG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDY
LKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
AKELAANTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKG
TEIISHAIANSDAFSIAGGGDTLAAINLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN

Sequences:

>Translated_386_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDY
LKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
AKELAANTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKG
TEIISHAIANSDAFSIAGGGDTLAAINLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN
>Mature_385_residues
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDYL
KNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIAC
AGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPVA
KELAANTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKGT
EIISHAIANSDAFSIAGGGDTLAAINLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=36.8932038834951, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=36.2530413625304, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1789294, Length=386, Percent_Identity=81.0880829015544, Blast_Score=625, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=39.9515738498789, Blast_Score=252, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=38.6634844868735, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=38.2566585956416, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=39.0243902439024, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40954; Mature: 40823

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFKPEDSLQPVVDYLKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEECEEECCCCCCC
DPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIACAGPLLAAELDALGKALKEP
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCC
ARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
AKELAANTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNA
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCC
KTVLWNGPVGVFEFPHFRKGTEIISHAIANSDAFSIAGGGDTLAAINLFGIADKISYIST
CEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHHHEEC
GGGAFLEFVEGKVLPAVEILEKRAKN
CCHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFKPEDSLQPVVDYLKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEECEEECCCCCCC
DPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIACAGPLLAAELDALGKALKEP
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCC
ARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
AKELAANTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNA
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCC
KTVLWNGPVGVFEFPHFRKGTEIISHAIANSDAFSIAGGGDTLAAINLFGIADKISYIST
CEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHHHHHHHHHEEC
GGGAFLEFVEGKVLPAVEILEKRAKN
CCHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]