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The map label for this gene is mnmG [H]

Identifier: 148825138

GI number: 148825138

Start: 11499

End: 13388

Strand: Direct

Name: mnmG [H]

Synonym: CGSHiEE_00050

Alternate gene names: 148825138

Gene position: 11499-13388 (Clockwise)

Preceding gene: 148825136

Following gene: 148825139

Centisome position: 0.63

GC content: 40.42

Gene sequence:

>1890_bases
ATGTTTTACACTGAAACTTATGATGTGATTGTGATCGGTGGTGGCCATGCGGGTACAGAAGCCGCGCTTGCACCAGCTCG
TATGGGATTTAAAACCCTTTTATTAACACATAATGTAGATACTTTAGGGCAAATGTCTTGTAACCCTGCAATTGGTGGGA
TCGGTAAAGGTCATTTAGTAAAAGAAGTAGATGCAATGGGCGGTTTAATGGCGCATGCTGCAGATAAAGCAGGGATCCAA
TTTCGTACTTTAAATAGCAGTAAAGGCCCAGCAGTGCGTGCTACTCGAGCTCAAGCTGACAGAGTTCTATATCGTCAAGC
TGTTCGTACTGCATTAGAAAATCAACCTAATTTAGATATTTTCCAACAAGAAGCGACCGATATTCTAATTGAGCAAGATC
GAGTTACAGGCGTTAGCACAAAAATGGGATTAATTTTTCGTGCTAAATCAGTGGTATTAACTGCGGGTACTTTCTTAGCC
GGTAAAATTCATATTGGTTTGGAAAATTATGAAGGTGGCCGTGCAGGGGACCCTGCTTCTGTAAATCTTTCACATCGATT
AAGAGATCTCGGATTACGTGTAGATCGCCTTAAAACGGGTACACCACCACGTATTGATGCGCGTACGATCAATTTTGATA
TTTTAGCTAAACAACATGGTGATGAGGTGTTGCCCATGTTCTCTTTTATGGGATCTGTTGATGATCACCCTCAACAAATT
CCTTGTTATATTACCTACACCAATGAACAAACTCATGAAGTGATCCGTAATAATTTGGATCGCAGTCCAATGTATACTGG
TGTGATTGAAGGGATTGGTCCACGTTATTGCCCATCCATTGAAGATAAAGTGATGCGTTTCTCGGATCGTAATTCACATC
AAATTTATTTAGAACCAGAAGGCTTAACCAGTAATGAGGTGTATCCAAACGGGATCTCTACCAGTTTACCGTTTGACGTG
CAAATGGGCATTGTGAATTCTATGAAAGGTTTAGAAAACGCACGTATTGTGAAACCAGGTTATGCCATTGAATACGATTA
TTTTGATCCACGCGATTTAAAACCAACATTAGAAACTAAATCAATTTCGGGTCTATTCTTCGCAGGTCAAATTAACGGTA
CAACCGGTTATGAAGAAGCAGCGGCACAAGGTTTATTGGCTGGGATTAACGCAGGTCTTTATGTTCAAGAGAAGGATGCA
TGGTATCCACGCCGTGATCAATCTTATACTGGCGTATTGGTGGATGACCTTTGCACACTTGGTACCAAAGAACCATATCG
CGTATTTACTTCTCGTGCAGAATACCGTTTGTTATTACGTGAAGATAATGCGGATATTCGTTTAACGCCAATTGCCCATG
AACTAGGTTTGATTGATGATGCTCGCTGGGCACGTTTCAATCAAAAAATGGAAAATATTGAACAGGAACGCCAACGCTTA
CGCAACATTTGGTTGCATCCGCGTTCTGAATATTTAGAAGAAGCCAATAAAGTGCTTGGTAGTCCACTTGTACGTGAGGC
TAGCGGTGAAGATTTATTGCGTCGTCCTGAAATGACCTACGATATTTTGACTTCTTTAACGCCATATAAACCGGCAATGG
AAGATAAGGAAGCTGTTGAGCAAGTAGAAATTGCTATTAAATATCAAGGCTATATCGAGCATCAACAAGAAGAAATCGAA
AAACAAAAACGCCACGAAAATACGGCTATTCCTGCTAACTTTGATTACAGCAAAGTGTCTAGTTTATCTAATGAAGTACG
TGCGAAATTGGAACAACATCGTCCGGTTTCCATTAGTCAAGCGAGCCGAATTTCAGGTATAACGCCTGCTGCTATTTCAA
TTATTCTAGTTAATTTGAAAAAACAAGGAATGCTAAAACGTGGAGAATAA

Upstream 100 bases:

>100_bases
ATCTTTCAAAGGATCAATAGATCCATTTCTTTTTTTGTCAGGTGTTTTAAATTTCTGTAGAATAACGCCAATTTTTTGAT
CCGAATAAATAGAGATAGAT

Downstream 100 bases:

>100_bases
AGTTTTTTATTAAAAAACAAAAGTGCGGTAAATTTTAATCTAGTTTTAATCCTAGAGCATATTGTTTAATTATTGACAAT
AAAGCGTAAAACTATTAGAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLIFRAKSVVLTAGTFLA
GKIHIGLENYEGGRAGDPASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPMFSFMGSVDDHPQQI
PCYITYTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIEQERQRL
RNIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSSLSNEVRAKLEQHRPVSISQASRISGITPAAISIILVNLKKQGMLKRGE

Sequences:

>Translated_629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLIFRAKSVVLTAGTFLA
GKIHIGLENYEGGRAGDPASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPMFSFMGSVDDHPQQI
PCYITYTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIEQERQRL
RNIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSSLSNEVRAKLEQHRPVSISQASRISGITPAAISIILVNLKKQGMLKRGE
>Mature_629_residues
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAHAADKAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEATDILIEQDRVTGVSTKMGLIFRAKSVVLTAGTFLA
GKIHIGLENYEGGRAGDPASVNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPMFSFMGSVDDHPQQI
PCYITYTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLTSNEVYPNGISTSLPFDV
QMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETKSISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDA
WYPRRDQSYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIEQERQRL
RNIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVEQVEIAIKYQGYIEHQQEEIE
KQKRHENTAIPANFDYSKVSSLSNEVRAKLEQHRPVSISQASRISGITPAAISIILVNLKKQGMLKRGE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=643, Percent_Identity=46.0342146189736, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI19882217, Length=668, Percent_Identity=44.311377245509, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI183227703, Length=683, Percent_Identity=43.3382137628111, Blast_Score=490, Evalue=1e-138,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=76.0765550239234, Blast_Score=1009, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=41.3183279742765, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=625, Percent_Identity=43.04, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=46.7817896389325, Blast_Score=526, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70244; Mature: 70244

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAHAADKAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCH
FQQEATDILIEQDRVTGVSTKMGLIFRAKSVVLTAGTFLAGKIHIGLENYEGGRAGDPAS
HHHCCHHEEEECCCCCCCCHHHEEEEEECEEEEEECCEEEEEEEEEEECCCCCCCCCCCE
VNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPMFSFMGSVDDHPQQI
EEHHHHHHHHCCEEEHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCC
PCYITYTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPE
CEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCC
GLTSNEVYPNGISTSLPFDVQMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCCCCC
SISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDAWYPRRDQSYTGVLVDDLCTL
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIEQERQRL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RNIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVE
HHHCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIEKQKRHENTAIPANFDYSKVSSLSNEVRAKLEQHRPVSISQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
ASRISGITPAAISIILVNLKKQGMLKRGE
HHHCCCCCHHHEEEEEEECHHCCCCCCCC
>Mature Secondary Structure
MFYTETYDVIVIGGGHAGTEAALAPARMGFKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAHAADKAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCH
FQQEATDILIEQDRVTGVSTKMGLIFRAKSVVLTAGTFLAGKIHIGLENYEGGRAGDPAS
HHHCCHHEEEECCCCCCCCHHHEEEEEECEEEEEECCEEEEEEEEEEECCCCCCCCCCCE
VNLSHRLRDLGLRVDRLKTGTPPRIDARTINFDILAKQHGDEVLPMFSFMGSVDDHPQQI
EEHHHHHHHHCCEEEHHCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCC
PCYITYTNEQTHEVIRNNLDRSPMYTGVIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPE
CEEEEECCCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCC
GLTSNEVYPNGISTSLPFDVQMGIVNSMKGLENARIVKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCCCCC
SISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQEKDAWYPRRDQSYTGVLVDDLCTL
CCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIEQERQRL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RNIWLHPRSEYLEEANKVLGSPLVREASGEDLLRRPEMTYDILTSLTPYKPAMEDKEAVE
HHHCCCCHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIEKQKRHENTAIPANFDYSKVSSLSNEVRAKLEQHRPVSISQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
ASRISGITPAAISIILVNLKKQGMLKRGE
HHHCCCCCHHHEEEEEEECHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA