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The map label for this gene is cpdB [H]

Identifier: 148825136

GI number: 148825136

Start: 8329

End: 10302

Strand: Direct

Name: cpdB [H]

Synonym: CGSHiEE_00040

Alternate gene names: 148825136

Gene position: 8329-10302 (Clockwise)

Preceding gene: 148825135

Following gene: 148825138

Centisome position: 0.46

GC content: 38.96

Gene sequence:

>1974_bases
ATGATGAACAGACGTCATTTTATTCAAATTGGTGCGACCAGTATTCTTGCATTAAGTGCAAACCGTTTTGCGATGGCAAA
AGGAAAGAGCGATGTTGATTTACGCATTGTGGCAACAACAGATGTTCATAGTTTCTTGACCGATTTTGACTATTATAAAG
ATGCACCAACCGATAAATTCGGTTTTACTCGTGCGGCAAGCCTTATTCGTCAAGCACGTGCTGAAGTAAAAAATAGCGTA
TTGGTAGATAATGGGGATTTAATTCAAGGTAATCCAATTGCAGACTATCAAGCCGCACAAGGCTATAAAGAGGGTAAATC
TAACCCTGCGGTCGATTGTTTAAATGCGATGAATTATGAAGTGGGTACATTAGGTAACCACGAATTTAACTATGGTCTCA
ATTATCTTGCAGATGCCATCAAACAAGCTAAATTCCCAATTGTGAATGCTAACGTAGTAAAAGCGGGGACAGAAGAACCT
TATTTCACACCTTATGTCATTCAAGAAAAATCTGTGGTGGATAATAATGGCAAAACACATAAATTAAAAATTGGTTACAT
CGGTTTTGTGCCACCGCAAATTATGGTTTGGGATAAAGCAAACCTTCAAGGCAAAGTTGAAACCCGTGATATTGTAAAAA
CAGCGCAAAAATATGTACCTGAAATGAAGAAAAAAGGGGCTGATATTGTCGTTGCATTAGCTCACACTGGTCCATCTGAT
GAACCTTATCAAGAAGGTGCTGAAAACTCAGCATTCTATCTAGCGGATGTTCCACACATTGATGCGGTTATTTTTGGTCA
CTCACACCGTTTATTCCCAAATAAAGAATTCGCAAAATCACCAAATGCAGACATCGTAAATGGTACCGTAAAAGGCGTAC
CAGAAAGTATGGCTGGTTACTGGGCAAATAATATCAGCGTGGTGGATTTAGGCTTAACAGAACACAAGGGTAAATGGATC
GTGACTTCTGGCAAAGCTGTGCTTCGTCCAATTTATGATGTAGAAACCAAAAAAGCACTTGCAAAAAATGACCCAGAAAT
TACCGCACTTTTAAAACCAGTTCACGAAGCAACACGTAAATATGTTTCTCAACCTATCGGCAAAGCCACAGACAATATGT
ATAGTTACCTTGCATTAATACAAGATGATCCAACCATTCAAATTGTAAACCAAGCACAAAAAGCGTATGTAGAGAAAGTT
GCACCAAGTGTTGCAGCAATGGCTGGCTTACCTATTTTAAGTGCAGGTGCTCCATTTAAAGCGGGTGGTCGTAAAAATGA
CCCAACGGGCTATACCGAAGTAAATAAAGGTGAATTAACTTTCCGTAATGCAGCAGACTTATACCTTTATCCAAATACGC
TAGTGGTTGTTAAAGCGACAGGCGAACAATTAAAAGAATGGTTAGAATGTAGTGCTGGTATGTTTAAACAAATTGACCCT
ACAAGTGATAAACCACAATCATTAATCGACTGGGAAGGTTTCCGCACTTATAACTTTGATGTGATTGATGGTGTCAATTA
TGAATACGATCTAACCAAACCAGCTCGTTACGATGGCGAATGTAAGTTAATCAACCCAGAATCGCATCGTGTAGTGAATC
TCACTTATCAAGGTAAACCAGTTGATCCAAAAGCAGAATTTTTGATTGCAACGAATAACTATCGTGCTTACGGCAATAAA
TTCCCAGGTACTGGCGATCAACATATTGTTTACGCATCACCAGATGAAAGCCGTCAAATTTTGGCTGATTATATTAAAGC
AACCAGTGAGAAAGAGGGTTCAGTCAATCCAAATGCGGATAAAAACTGGCGTTTTGTGCCTATCACAGGTAACGATAAAT
TAGATGTCCGTTTTGAAACATCGCCAAGCGAACAAGCGGCGAAATTTATCGCAGAAAAAGCACAATACCCAATGAAACAA
GTGGGTACTGACGAAATCGGTTTTGCAGTGTATCAAATTGATTTATCTAAATAA

Upstream 100 bases:

>100_bases
ATCTTAATTAAATAAAAATATTTTTACTCCTTTCTTTTAAGAATCCGCTATTTTTCTTAGAATAAAGGGAATATTTACTC
ACCCTAATAAGGAAAACTCT

Downstream 100 bases:

>100_bases
TAATTAGGAAAATAAAATGCCATCTAAATATTAAAATTTAGGTGGCATTTTTTATTCCTAAAGTGCGGTCAAATTTTTAG
TAGTTTTTTTACATATTGAA

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MMNRRHFIQIGATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAVDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNANVVKAGTEEP
YFTPYVIQEKSVVDNNGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGVPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDVETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALIQDDPTIQIVNQAQKAYVEKV
APSVAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGELTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDQHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK

Sequences:

>Translated_657_residues
MMNRRHFIQIGATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAVDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNANVVKAGTEEP
YFTPYVIQEKSVVDNNGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGVPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDVETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALIQDDPTIQIVNQAQKAYVEKV
APSVAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGELTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDQHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK
>Mature_657_residues
MMNRRHFIQIGATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKFGFTRAASLIRQARAEVKNSV
LVDNGDLIQGNPIADYQAAQGYKEGKSNPAVDCLNAMNYEVGTLGNHEFNYGLNYLADAIKQAKFPIVNANVVKAGTEEP
YFTPYVIQEKSVVDNNGKTHKLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGVPESMAGYWANNISVVDLGLTEHKGKWI
VTSGKAVLRPIYDVETKKALAKNDPEITALLKPVHEATRKYVSQPIGKATDNMYSYLALIQDDPTIQIVNQAQKAYVEKV
APSVAAMAGLPILSAGAPFKAGGRKNDPTGYTEVNKGELTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKPVDPKAEFLIATNNYRAYGNK
FPGTGDQHIVYASPDESRQILADYIKATSEKEGSVNPNADKNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQ
VGTDEIGFAVYQIDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=640, Percent_Identity=62.65625, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=605, Percent_Identity=22.9752066115702, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922444, Length=240, Percent_Identity=26.25, Blast_Score=66, Evalue=1e-10,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72649; Mature: 72649

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRRHFIQIGATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKF
CCCCCEEEEECCEEEEEEECCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCC
GFTRAASLIRQARAEVKNSVLVDNGDLIQGNPIADYQAAQGYKEGKSNPAVDCLNAMNYE
CHHHHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHCCHHCCCCCCHHHHHHHCCCC
VGTLGNHEFNYGLNYLADAIKQAKFPIVNANVVKAGTEEPYFTPYVIQEKSVVDNNGKTH
EECCCCCCCCCCHHHHHHHHHHCCCCEECCEEEECCCCCCCCCCEEEECCEEECCCCCEE
KLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGVPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCEECCCCCCCCHHHHHH
WANNISVVDLGLTEHKGKWIVTSGKAVLRPIYDVETKKALAKNDPEITALLKPVHEATRK
HCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVSQPIGKATDNMYSYLALIQDDPTIQIVNQAQKAYVEKVAPSVAAMAGLPILSAGAPFK
HHHCCCCHHHHHHEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AGGRKNDPTGYTEVNKGELTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
CCCCCCCCCCCEECCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCHHCCCC
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKP
CCCCCCHHCCCCCCEEECEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEEECCCC
VDPKAEFLIATNNYRAYGNKFPGTGDQHIVYASPDESRQILADYIKATSEKEGSVNPNAD
CCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
KNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQVGTDEIGFAVYQIDLSK
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MMNRRHFIQIGATSILALSANRFAMAKGKSDVDLRIVATTDVHSFLTDFDYYKDAPTDKF
CCCCCEEEEECCEEEEEEECCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCC
GFTRAASLIRQARAEVKNSVLVDNGDLIQGNPIADYQAAQGYKEGKSNPAVDCLNAMNYE
CHHHHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHCCHHCCCCCCHHHHHHHCCCC
VGTLGNHEFNYGLNYLADAIKQAKFPIVNANVVKAGTEEPYFTPYVIQEKSVVDNNGKTH
EECCCCCCCCCCHHHHHHHHHHCCCCEECCEEEECCCCCCCCCCEEEECCEEECCCCCEE
KLKIGYIGFVPPQIMVWDKANLQGKVETRDIVKTAQKYVPEMKKKGADIVVALAHTGPSD
EEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EPYQEGAENSAFYLADVPHIDAVIFGHSHRLFPNKEFAKSPNADIVNGTVKGVPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHCCCCCCEECCCCCCCCHHHHHH
WANNISVVDLGLTEHKGKWIVTSGKAVLRPIYDVETKKALAKNDPEITALLKPVHEATRK
HCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVSQPIGKATDNMYSYLALIQDDPTIQIVNQAQKAYVEKVAPSVAAMAGLPILSAGAPFK
HHHCCCCHHHHHHEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AGGRKNDPTGYTEVNKGELTFRNAADLYLYPNTLVVVKATGEQLKEWLECSAGMFKQIDP
CCCCCCCCCCCEECCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCHHCCCC
TSDKPQSLIDWEGFRTYNFDVIDGVNYEYDLTKPARYDGECKLINPESHRVVNLTYQGKP
CCCCCCHHCCCCCCEEECEEEECCCCCEEECCCCCCCCCEEEEECCCCCEEEEEEECCCC
VDPKAEFLIATNNYRAYGNKFPGTGDQHIVYASPDESRQILADYIKATSEKEGSVNPNAD
CCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCC
KNWRFVPITGNDKLDVRFETSPSEQAAKFIAEKAQYPMKQVGTDEIGFAVYQIDLSK
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]