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The map label for this gene is ispB [C]

Identifier: 148824606

GI number: 148824606

Start: 3826144

End: 3827223

Strand: Reverse

Name: ispB [C]

Synonym: TBFG_13433

Alternate gene names: 148824606

Gene position: 3827223-3826144 (Counterclockwise)

Preceding gene: 148824610

Following gene: 148824605

Centisome position: 86.5

GC content: 65.19

Gene sequence:

>1080_bases
ATGCGCGGTACCGACGAAAAGTACGGACTGCCACCGCAACCCGACAGCGACCGTATGACCCGTCGCACGCTTCCCGTTTT
GGGACTCGCACACGAACTCATCACACCGACGCTGCGCCAGATGGCTGACCGCCTCGATCCCCACATGCGGCCCGTCGTGT
CCTACCACCTCGGCTGGTCTGATGAACGCGGCCGTCCAGTGAACAACAACTGCGGCAAGGCAATCCGTCCTGCTTTGGTG
TTTGTCGCCGCCGAAGCCGCCGGCGCCGACCCCCACTCGGCGATTCCCGGAGCCGTCTCGGTTGAACTGGTGCACAACTT
CTCGCTCGTCCATGACGACCTCATGGACCGCGACGAGCACCGTCGGCACCGGCCGACGGTGTGGGCACTGTGGGGCGATG
CGATGGCGCTATTGGCCGGTGATGCGATGCTGTCGCTGGCGCACGAGGTCCTGCTGGATTGCGATTCACCACACGTCGGT
GCGGCGCTGCGCGCGATTTCGGAAGCCACCCGTGAGTTGATCCGTGGGCAGGCCGCCGACACCGCGTTCGAAAGCCGAAC
CGACGTCGCACTCGATGAGTGTCTCAAGATGGCTGAAGGCAAGACCGCAGCACTGATGGCAGCCAGCGCCGAGGTCGGCG
CGTTACTCGCTGGCGCACCGCGTTCGGTGCGAGAAGCGTTGGTTGCCTACGGTCGTCACATTGGACTTGCCTTCCAATTG
GTCGACGACCTGCTCGGCATTTGGGGCCGTCCTGAGATCACCGGCAAGCCGGTGTACTCGGACCTTCGCTCCCGCAAGAA
AACTCTGCCCGTTACCTGGACCGTGGCGCACGGCGGTTCGGCCGGCCGCCGCCTCGCAGCGTGGCTGGTGGACGAGACGG
GCAGCCAGACAGCGTCCGATGATGAGCTCGCAGCGGTCGCAGAACTGATCGAGTGCGGTGGGGGGCGCAGGTGGGCAAGC
GCGGAGGCCCGCCGACACGTAACGCAAGGCATCGACATGGTGGCGCGCATCGGCATTCCCGATCGCCCGGCCGCTGAGCT
TCAGGATTTGGCTCACTACATCGTCGATCGTCAGGCGTGA

Upstream 100 bases:

>100_bases
CTCCAATAGTGCCTCCGCCATTGCACATTGTGCACATTTTCGCGTATTGCTGGGCAACTTGCCCATGGGCCGTGCCATGA
GCGAATGCTAGATTCACGCA

Downstream 100 bases:

>100_bases
CTGACCAACACCGTAGTTGGAGGACGGCATGACCGAGATCGAACAGGCGTATCGAATCACCGAGTCGATCACGCGCACGG
CCGCCAGAAACTTCTACTAC

Product: multi-functional geranylgeranyl pyrophosphate synthetase idsA1: dimethylallyltransferase + geranyltranstransferase + farnesyltranstransferase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MRGTDEKYGLPPQPDSDRMTRRTLPVLGLAHELITPTLRQMADRLDPHMRPVVSYHLGWSDERGRPVNNNCGKAIRPALV
FVAAEAAGADPHSAIPGAVSVELVHNFSLVHDDLMDRDEHRRHRPTVWALWGDAMALLAGDAMLSLAHEVLLDCDSPHVG
AALRAISEATRELIRGQAADTAFESRTDVALDECLKMAEGKTAALMAASAEVGALLAGAPRSVREALVAYGRHIGLAFQL
VDDLLGIWGRPEITGKPVYSDLRSRKKTLPVTWTVAHGGSAGRRLAAWLVDETGSQTASDDELAAVAELIECGGGRRWAS
AEARRHVTQGIDMVARIGIPDRPAAELQDLAHYIVDRQA

Sequences:

>Translated_359_residues
MRGTDEKYGLPPQPDSDRMTRRTLPVLGLAHELITPTLRQMADRLDPHMRPVVSYHLGWSDERGRPVNNNCGKAIRPALV
FVAAEAAGADPHSAIPGAVSVELVHNFSLVHDDLMDRDEHRRHRPTVWALWGDAMALLAGDAMLSLAHEVLLDCDSPHVG
AALRAISEATRELIRGQAADTAFESRTDVALDECLKMAEGKTAALMAASAEVGALLAGAPRSVREALVAYGRHIGLAFQL
VDDLLGIWGRPEITGKPVYSDLRSRKKTLPVTWTVAHGGSAGRRLAAWLVDETGSQTASDDELAAVAELIECGGGRRWAS
AEARRHVTQGIDMVARIGIPDRPAAELQDLAHYIVDRQA
>Mature_359_residues
MRGTDEKYGLPPQPDSDRMTRRTLPVLGLAHELITPTLRQMADRLDPHMRPVVSYHLGWSDERGRPVNNNCGKAIRPALV
FVAAEAAGADPHSAIPGAVSVELVHNFSLVHDDLMDRDEHRRHRPTVWALWGDAMALLAGDAMLSLAHEVLLDCDSPHVG
AALRAISEATRELIRGQAADTAFESRTDVALDECLKMAEGKTAALMAASAEVGALLAGAPRSVREALVAYGRHIGLAFQL
VDDLLGIWGRPEITGKPVYSDLRSRKKTLPVTWTVAHGGSAGRRLAAWLVDETGSQTASDDELAAVAELIECGGGRRWAS
AEARRHVTQGIDMVARIGIPDRPAAELQDLAHYIVDRQA

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=209, Percent_Identity=32.5358851674641, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789578, Length=298, Percent_Identity=30.2013422818792, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786623, Length=252, Percent_Identity=34.1269841269841, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=236, Percent_Identity=30.9322033898305, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24651612, Length=210, Percent_Identity=34.2857142857143, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GGPPS_MYCBO (P0A5H9)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857072.1
- ProteinModelPortal:   P0A5H9
- SMR:   P0A5H9
- EnsemblBacteria:   EBMYCT00000016735
- GeneID:   1090991
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3431c
- GeneTree:   EBGT00050000015046
- HOGENOM:   HBG732667
- OMA:   RMAPLES
- ProtClustDB:   CLSK799990
- BioCyc:   MBOV233413:MB3431C-MONOMER
- BRENDA:   2.5.1.1
- BRENDA:   2.5.1.10
- BRENDA:   2.5.1.29
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10

Molecular weight: Translated: 38853; Mature: 38853

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 73-73 BINDING 76-76 BINDING 105-105 BINDING 121-121 BINDING 122-122 BINDING 201-201 BINDING 202-202 BINDING 239-239 BINDING 256-256 BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTDEKYGLPPQPDSDRMTRRTLPVLGLAHELITPTLRQMADRLDPHMRPVVSYHLGWS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCC
DERGRPVNNNCGKAIRPALVFVAAEAAGADPHSAIPGAVSVELVHNFSLVHDDLMDRDEH
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHH
RRHRPTVWALWGDAMALLAGDAMLSLAHEVLLDCDSPHVGAALRAISEATRELIRGQAAD
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
TAFESRTDVALDECLKMAEGKTAALMAASAEVGALLAGAPRSVREALVAYGRHIGLAFQL
HHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VDDLLGIWGRPEITGKPVYSDLRSRKKTLPVTWTVAHGGSAGRRLAAWLVDETGSQTASD
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCH
DELAAVAELIECGGGRRWASAEARRHVTQGIDMVARIGIPDRPAAELQDLAHYIVDRQA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGTDEKYGLPPQPDSDRMTRRTLPVLGLAHELITPTLRQMADRLDPHMRPVVSYHLGWS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCC
DERGRPVNNNCGKAIRPALVFVAAEAAGADPHSAIPGAVSVELVHNFSLVHDDLMDRDEH
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHH
RRHRPTVWALWGDAMALLAGDAMLSLAHEVLLDCDSPHVGAALRAISEATRELIRGQAAD
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
TAFESRTDVALDECLKMAEGKTAALMAASAEVGALLAGAPRSVREALVAYGRHIGLAFQL
HHHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VDDLLGIWGRPEITGKPVYSDLRSRKKTLPVTWTVAHGGSAGRRLAAWLVDETGSQTASD
HHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCH
DELAAVAELIECGGGRRWASAEARRHVTQGIDMVARIGIPDRPAAELQDLAHYIVDRQA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972