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The map label for this gene is guaA

Identifier: 148824604

GI number: 148824604

Start: 3823618

End: 3825195

Strand: Reverse

Name: guaA

Synonym: TBFG_13431

Alternate gene names: 148824604

Gene position: 3825195-3823618 (Counterclockwise)

Preceding gene: 148824605

Following gene: 148824603

Centisome position: 86.46

GC content: 67.17

Gene sequence:

>1578_bases
GTGGTGCAGCCTGCTGACATCGACGTGCCTGAGACACCGGCTCGACCGGTGTTGGTGGTCGACTTCGGTGCCCAATATGC
CCAGTTGATTGCCCGTCGGGTTCGAGAAGCACGGGTTTTCTCCGAGGTGATTCCGCACACCGCCTCGATCGAGGAGATCA
GGGCCCGTCAGCCGGTGGCGCTCGTGCTTTCCGGTGGACCGGCCAGTGTCTACGCCGACGGCGCTCCGAAACTCGATCCG
GCGCTACTGGACCTCGGTGTACCGGTTCTTGGCATCTGCTACGGGTTTCAGGCCATGGCGCAGGCGCTCGGGGGGATCGT
CGCCCACACCGGCACTCGCGAATACGGTCGGACTGAACTGAAAGTCCTTGGTGGCAAACTGCATTCGGATCTTCCCGAGG
TTCAGCCGGTATGGATGAGTCACGGTGACGCGGTCACGGCCGCGCCGGACGGATTCGACGTGGTGGCTAGCAGCGCCGGT
GCCCCGGTGGCCGCCTTCGAGGCCTTCGACCGGCGCCTGGCCGGGGTGCAGTATCACCCGGAGGTGATGCACACCCCACA
CGGGCAACAGGTGCTCAGCCGGTTTTTGCACGACTTCGCCGGGCTCGGCGCCCAGTGGACGCCCGCCAACATCGCCAACG
CGCTGATCGAGCAGGTGCGCACCCAGATCGGCGACGGCCACGCCATCTGCGGGCTATCCGGCGGCGTGGATTCCGCGGTG
GCCGCGGCCCTGGTGCAGCGGGCCATCGGCGACCGGTTGACCTGTGTCTTCGTCGACCACGGGCTGTTGCGCGCCGGTGA
GCGGGCGCAGGTGCAACGCGATTTCGTGGCCGCCACCGGCGCCAACCTGGTCACCGTCGACGCGGCCGAGACCTTCCTCG
AGGCGCTGTCGGGCGTGAGCGCCCCCGAGGGCAAACGCAAGATCATCGGCCGTCAGTTCATCCGCGCGTTCGAGGGCGCG
GTGCGGGATGTGCTGGACGGTAAGACTGCTGAGTTCCTGGTGCAGGGCACGCTGTATCCGGATGTGGTGGAGTCCGGCGG
GGGCAGCGGCACCGCGAACATCAAGAGCCACCACAATGTCGGCGGCCTGCCCGACGACCTGAAGTTCACCCTCGTTGAGC
CGCTGCGGCTGCTGTTCAAAGACGAGGTGCGCGCGGTCGGGCGGGAGTTGGGTCTGCCGGAGGAGATCGTGGCGCGCCAG
CCGTTTCCGGGACCGGGGTTGGGTATTCGGATCGTCGGGGAGGTCACCGCCAAGCGGTTGGATACGCTGCGGCACGCCGA
TTCGATCGTGCGTGAGGAGCTGACCGCGGCGGGCCTGGACAACCAGATCTGGCAGTGTCCGGTGGTGCTGTTGGCCGATG
TCCGCTCGGTGGGTGTGCAGGGTGACGGTCGCACCTATGGCCACCCGATTGTGCTGCGTCCGGTGTCGAGTGAAGACGCG
ATGACCGCCGACTGGACCCGGGTGCCCTACGAGGTGCTCGAGCGCATCTCGACCCGGATCACCAACGAGGTCGCCGAGGT
CAACCGCGTGGTGCTGGACATCACCAGCAAGCCGCCCGCCACCATCGAGTGGGAGTAG

Upstream 100 bases:

>100_bases
TGGCGGCGGCAGCACTGGCCTCTTCGGTAACCTGCGGACCGGCCCATGGACCGCTACCGGCCGACCTCGGGAGTCACCCC
AGCCACTAGACTGACTGGCC

Downstream 100 bases:

>100_bases
CGGCGGCCAGCATTAGCCAGGCCCGGCTTTTGTCACAGGCGACACATTGGTATCTGTCGGGATGAATACGCGTTGTGCCC
GCCGGCCCAGAGATTGCTGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MVQPADIDVPETPARPVLVVDFGAQYAQLIARRVREARVFSEVIPHTASIEEIRARQPVALVLSGGPASVYADGAPKLDP
ALLDLGVPVLGICYGFQAMAQALGGIVAHTGTREYGRTELKVLGGKLHSDLPEVQPVWMSHGDAVTAAPDGFDVVASSAG
APVAAFEAFDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLGAQWTPANIANALIEQVRTQIGDGHAICGLSGGVDSAV
AAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLVTVDAAETFLEALSGVSAPEGKRKIIGRQFIRAFEGA
VRDVLDGKTAEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDLKFTLVEPLRLLFKDEVRAVGRELGLPEEIVARQ
PFPGPGLGIRIVGEVTAKRLDTLRHADSIVREELTAAGLDNQIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
MTADWTRVPYEVLERISTRITNEVAEVNRVVLDITSKPPATIEWE

Sequences:

>Translated_525_residues
MVQPADIDVPETPARPVLVVDFGAQYAQLIARRVREARVFSEVIPHTASIEEIRARQPVALVLSGGPASVYADGAPKLDP
ALLDLGVPVLGICYGFQAMAQALGGIVAHTGTREYGRTELKVLGGKLHSDLPEVQPVWMSHGDAVTAAPDGFDVVASSAG
APVAAFEAFDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLGAQWTPANIANALIEQVRTQIGDGHAICGLSGGVDSAV
AAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLVTVDAAETFLEALSGVSAPEGKRKIIGRQFIRAFEGA
VRDVLDGKTAEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDLKFTLVEPLRLLFKDEVRAVGRELGLPEEIVARQ
PFPGPGLGIRIVGEVTAKRLDTLRHADSIVREELTAAGLDNQIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
MTADWTRVPYEVLERISTRITNEVAEVNRVVLDITSKPPATIEWE
>Mature_525_residues
MVQPADIDVPETPARPVLVVDFGAQYAQLIARRVREARVFSEVIPHTASIEEIRARQPVALVLSGGPASVYADGAPKLDP
ALLDLGVPVLGICYGFQAMAQALGGIVAHTGTREYGRTELKVLGGKLHSDLPEVQPVWMSHGDAVTAAPDGFDVVASSAG
APVAAFEAFDRRLAGVQYHPEVMHTPHGQQVLSRFLHDFAGLGAQWTPANIANALIEQVRTQIGDGHAICGLSGGVDSAV
AAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLVTVDAAETFLEALSGVSAPEGKRKIIGRQFIRAFEGA
VRDVLDGKTAEFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDLKFTLVEPLRLLFKDEVRAVGRELGLPEEIVARQ
PFPGPGLGIRIVGEVTAKRLDTLRHADSIVREELTAAGLDNQIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
MTADWTRVPYEVLERISTRITNEVAEVNRVVLDITSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=546, Percent_Identity=35.1648351648352, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1788854, Length=520, Percent_Identity=48.8461538461538, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1789760, Length=155, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71992717, Length=568, Percent_Identity=35.5633802816901, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71992710, Length=568, Percent_Identity=35.5633802816901, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI133901714, Length=568, Percent_Identity=35.5633802816901, Blast_Score=286, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6323873, Length=515, Percent_Identity=46.9902912621359, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI281365319, Length=543, Percent_Identity=32.9650092081031, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_MYCBO (P0A5A2)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857070.1
- ProteinModelPortal:   P0A5A2
- EnsemblBacteria:   EBMYCT00000015979
- GeneID:   1090986
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3429c
- GeneTree:   EBGT00050000015860
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   PRK00074
- BioCyc:   MBOV233413:MB3429C-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56060; Mature: 56060

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 93-93 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQPADIDVPETPARPVLVVDFGAQYAQLIARRVREARVFSEVIPHTASIEEIRARQPVA
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
LVLSGGPASVYADGAPKLDPALLDLGVPVLGICYGFQAMAQALGGIVAHTGTREYGRTEL
EEEECCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHH
KVLGGKLHSDLPEVQPVWMSHGDAVTAAPDGFDVVASSAGAPVAAFEAFDRRLAGVQYHP
HHHCCHHHCCCCCCCCHHHCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCEECC
EVMHTPHGQQVLSRFLHDFAGLGAQWTPANIANALIEQVRTQIGDGHAICGLSGGVDSAV
HHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
AAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLVTVDAAETFLEALSGVS
HHHHHHHHHCCCEEEEEECCHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCC
APEGKRKIIGRQFIRAFEGAVRDVLDGKTAEFLVQGTLYPDVVESGGGSGTANIKSHHNV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCC
GGLPDDLKFTLVEPLRLLFKDEVRAVGRELGLPEEIVARQPFPGPGLGIRIVGEVTAKRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHH
DTLRHADSIVREELTAAGLDNQIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
MTADWTRVPYEVLERISTRITNEVAEVNRVVLDITSKPPATIEWE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MVQPADIDVPETPARPVLVVDFGAQYAQLIARRVREARVFSEVIPHTASIEEIRARQPVA
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEE
LVLSGGPASVYADGAPKLDPALLDLGVPVLGICYGFQAMAQALGGIVAHTGTREYGRTEL
EEEECCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHH
KVLGGKLHSDLPEVQPVWMSHGDAVTAAPDGFDVVASSAGAPVAAFEAFDRRLAGVQYHP
HHHCCHHHCCCCCCCCHHHCCCCEEEECCCCHHHHHCCCCCCHHHHHHHHHHHCCCEECC
EVMHTPHGQQVLSRFLHDFAGLGAQWTPANIANALIEQVRTQIGDGHAICGLSGGVDSAV
HHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
AAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLVTVDAAETFLEALSGVS
HHHHHHHHHCCCEEEEEECCHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCC
APEGKRKIIGRQFIRAFEGAVRDVLDGKTAEFLVQGTLYPDVVESGGGSGTANIKSHHNV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCC
GGLPDDLKFTLVEPLRLLFKDEVRAVGRELGLPEEIVARQPFPGPGLGIRIVGEVTAKRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHH
DTLRHADSIVREELTAAGLDNQIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
MTADWTRVPYEVLERISTRITNEVAEVNRVVLDITSKPPATIEWE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972