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The map label for this gene is sigF [H]

Identifier: 148824488

GI number: 148824488

Start: 3679884

End: 3680669

Strand: Reverse

Name: sigF [H]

Synonym: TBFG_13315

Alternate gene names: 148824488

Gene position: 3680669-3679884 (Counterclockwise)

Preceding gene: 148824489

Following gene: 148824481

Centisome position: 83.19

GC content: 64.5

Gene sequence:

>786_bases
GTGACGGCGCGCGCTGCCGGCGGTTCTGCATCGCGAGCTAACGAATACGCCGACGTTCCGGAGATGTTTCGCGAGCTGGT
TGGTTTGCCTGCCGGCTCACCGGAATTCCAGCGGCACCGGGACAAGATCGTTCAGCGGTGCTTGCCGCTGGCCGATCACA
TCGCGCGGCGGTTCGAGGGTCGCGGCGAACCGCGTGACGACCTTATTCAGGTCGCGCGGGTCGGGCTGGTCAACGCCGCG
GTTCGCTTCGACGTGAAGACCGGGTCGGACTTCGTCTCCTTCGCGGTTCCTACCATCATGGGCGAGGTCCGACGACACTT
CCGCGACAACAGCTGGTCGGTCAAGGTTCCCCGGCGTCTCAAGGAACTGCATCTGCGGCTAGGTACCGCCACCGCCGATT
TGTCGCAGCGGCTCGGGCGGGCGCCGTCGGCATCGGAGCTCGCCGCGGAGCTCGGGATGGACCGCGCTGAGGTTATCGAA
GGTTTGCTGGCGGGTAGTTCCTACCACACCTTGTCCATCGACAGCGGTGGCGGCAGCGACGACGATGCCCGCGCAATCAC
AGACACCCTGGGCGACGTGGATGCGGGTCTTGACCAGATCGAGAATCGGGAGGTGCTTCGTCCGTTGCTCGAGGCGTTGC
CCGAGCGGGAACGAACGGTCTTGGTGCTCAGGTTCTTCGACTCGATGACCCAAACGCAGATCGCCGAGCGCGTCGGTATC
TCACAGATGCACGTGTCGCGGCTGCTGGCCAAGTCATTGGCACGGCTACGGGATCAGTTGGAGTAG

Upstream 100 bases:

>100_bases
TGGCCGACGACGTCCAGACCTTCCACGACGGTCGCCAGCCCGATGTAGCCGGCAGTGTCTTCGGCATCACGTTGACCGCC
CGACGGGCGGCATCCAGCAG

Downstream 100 bases:

>100_bases
CCGCCGGGCTTACTTGATCTCGGCGAGCACCGTGCCCTGGGTGATGGCCGCGCCCGCCTCGACCGCCAGCCCGGTGATGG
TGCCATCCTTATGCGCGGTG

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQVARVGLVNAA
VRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIE
GLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGI
SQMHVSRLLAKSLARLRDQLE

Sequences:

>Translated_261_residues
MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQVARVGLVNAA
VRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIE
GLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGI
SQMHVSRLLAKSLARLRDQLE
>Mature_260_residues
TARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQVARVGLVNAAV
RFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIEG
LLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGIS
QMHVSRLLAKSLARLRDQLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=227, Percent_Identity=29.0748898678414, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=27.8969957081545, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=25.7383966244726, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28794; Mature: 28663

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEG
CCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGEPRDDLIQVARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRL
CCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
KELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSD
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
DDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCH
SQMHVSRLLAKSLARLRDQLE
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEG
CCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGEPRDDLIQVARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRL
CCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
KELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSD
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCC
DDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCH
SQMHVSRLLAKSLARLRDQLE
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]