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The map label for this gene is birA [H]

Identifier: 148824481

GI number: 148824481

Start: 3672990

End: 3673790

Strand: Reverse

Name: birA [H]

Synonym: TBFG_13308

Alternate gene names: 148824481

Gene position: 3673790-3672990 (Counterclockwise)

Preceding gene: 148824488

Following gene: 148824480

Centisome position: 83.03

GC content: 67.67

Gene sequence:

>801_bases
GTGACCGACCGCGATCGGCTCAGGCCGCCTTTGGACGAACGCTCACTGCGCGACCAGCTGATCGGTGCTGGATCCGGGTG
GCGCCAACTTGACGTCGTGGCCCAAACCGGTTCCACCAACGCCGACTTGCTGGCCCGGGCGGCATCCGGGGCCGATATCG
ACGGGGTGGTGTTGATCGCCGAGCATCAGACCGCTGGGCGGGGGCGTCATGGCCGCGGCTGGGCGGCCACTGCCCGAGCG
CAGATCATCCTGTCGGTCGGTGTGCGTGTGGTCGACGTCCCGGTGCAAGCGTGGGGCTGGCTGTCACTGGCCGCGGGTCT
GGCGGTGCTCGATTCGGTGGCCCCCCTGATTGCAGTCCCGCCGGCCGAAACGGGCCTCAAGTGGCCCAATGACGTGCTGG
CCCGGGGTGGCAAGTTAGCCGGCATCCTGGCCGAGGTCGCGCAGCCGTTTGTGGTACTCGGTGTGGGACTCAACGTCACC
CAGGCCCCCGAAGAGGTTGATCCCGACGCGACCTCATTGCTGGACCTCGGAGTGGCGGCACCGGACCGCAACCGCATAGC
CAGCAGGCTGTTGCGCGAGCTCGAAGCTCGGATCATCCAGTGGCGCAACGCCAACCCACAACTGGCGGCCGACTACCGTG
CTCGCAGCCTGACCATTGGGTCGCGCGTGCGCGTCGAGCTCCCCGGTGGCCAGGACGTCGTCGGGATCGCGCGTGACATC
GATGACCAGGGTCGGCTGTGTCTGGACGTCGGCGGTCGAACGGTAGTTGTTTCAGCGGGCGACGTGGTGCATTTGCGTTA
A

Upstream 100 bases:

>100_bases
CTCGGTGGACCGCTCTGCGGAATGAGCCGAGCGGTCGGTAACGCTTGTCATGGGAGTCGATGTTATCGGCTGCCGCACAC
TACTTCCTTAGGCTGGGGCG

Downstream 100 bases:

>100_bases
CTCGCGCGGAGCTGGCGTCCCCAAAAGATTAAGGTCGCGGGCATGAGCTATCCGGAGAATGTCCTGGCCGCTGGCGAGCA
GGTCGTTCTGCACCGCCATC

Product: bifunctional protein birA: biotin operon repressor + biotin-[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTDRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASGADIDGVVLIAEHQTAGRGRHGRGWAATARA
QIILSVGVRVVDVPVQAWGWLSLAAGLAVLDSVAPLIAVPPAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVT
QAPEEVDPDATSLLDLGVAAPDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPGGQDVVGIARDI
DDQGRLCLDVGGRTVVVSAGDVVHLR

Sequences:

>Translated_266_residues
MTDRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASGADIDGVVLIAEHQTAGRGRHGRGWAATARA
QIILSVGVRVVDVPVQAWGWLSLAAGLAVLDSVAPLIAVPPAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVT
QAPEEVDPDATSLLDLGVAAPDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPGGQDVVGIARDI
DDQGRLCLDVGGRTVVVSAGDVVHLR
>Mature_265_residues
TDRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASGADIDGVVLIAEHQTAGRGRHGRGWAATARAQ
IILSVGVRVVDVPVQAWGWLSLAAGLAVLDSVAPLIAVPPAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVTQ
APEEVDPDATSLLDLGVAAPDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPGGQDVVGIARDID
DQGRLCLDVGGRTVVVSAGDVVHLR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=249, Percent_Identity=33.7349397590361, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28121; Mature: 27990

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASGADIDGVVLIA
CCCCHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEE
EHQTAGRGRHGRGWAATARAQIILSVGVRVVDVPVQAWGWLSLAAGLAVLDSVAPLIAVP
ECCCCCCCCCCCCCCHHHHHHHHEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEC
PAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVTQAPEEVDPDATSLLDLGVAA
CCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCC
PDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPGGQDVVGIARDI
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCEEEEECCCCCCEEEHHHCC
DDQGRLCLDVGGRTVVVSAGDVVHLR
CCCCCEEEEECCCEEEEECCCEEEEC
>Mature Secondary Structure 
TDRDRLRPPLDERSLRDQLIGAGSGWRQLDVVAQTGSTNADLLARAASGADIDGVVLIA
CCCHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEE
EHQTAGRGRHGRGWAATARAQIILSVGVRVVDVPVQAWGWLSLAAGLAVLDSVAPLIAVP
ECCCCCCCCCCCCCCHHHHHHHHEECCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEC
PAETGLKWPNDVLARGGKLAGILAEVAQPFVVLGVGLNVTQAPEEVDPDATSLLDLGVAA
CCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCC
PDRNRIASRLLRELEARIIQWRNANPQLAADYRARSLTIGSRVRVELPGGQDVVGIARDI
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCEEEEECCCCCCEEEHHHCC
DDQGRLCLDVGGRTVVVSAGDVVHLR
CCCCCEEEEECCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]