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The map label for this gene is cobB

Identifier: 148824038

GI number: 148824038

Start: 3167786

End: 3169159

Strand: Reverse

Name: cobB

Synonym: TBFG_12863

Alternate gene names: 148824038

Gene position: 3169159-3167786 (Counterclockwise)

Preceding gene: 148824039

Following gene: 148824037

Centisome position: 71.63

GC content: 68.05

Gene sequence:

>1374_bases
ATGCGGGTATCCGCGGTGGCCGTCGCCGCGCCTGCGTCGGGCAGCGGTAAGACCACGATCGCGACGGGCTTGATCGGAGC
GCTGCGGCAGGCCGGTCACACCGTCGCGCCGTTTAAGGTAGGCCCGGATTTTATCGACCCCGGCTATCACGCCCTGGCCG
CGGGACGGCCCGGCCGCAATCTCGACCCGGTACTGGTGGGGGAGCGGCTTATCGGCCCCCTGTACGCGCATGGAGTTGCG
GGCGCGGACATCGCCGTGATCGAAGGGGTGCTGGGGCTGTTCGACGGGCGCATTGGGCCTGCCGGGGGCGCGCCCGCAGC
GGGGTCCACCGCGCACGTCGCTGCTCTGCTTGGCGCCCCGGTGATCCTGGTGGTCGATGCCCGCGGCCAGAGTCACAGCG
TTGCCGCACTGCTGCACGGCTTTTCCACGTTCGACACCGCAACTCGGATCGCCGGTGTCATCCTCAACCGGGTCGGATCG
GCCCGACATGAACAGGTGCTGCGACAGGCGTGTGACCAGGCCGGTGTCGCGGTCTTGGGCGCCATTCCACGCACAGCTGA
ACTAGAGCTGCCGACAAGGTATCTGGGTCTGGTTACCGCCGTCGAGTACGGCCGTCGCGCACGGCTCGCCGTGCAGGCGA
TGACTGCTGTGGTCGCTCGCCACGTCGATCTGGCCGCGGTGATCGCCTGCGCCGGGAGCCAGGCGGCCCACCCGCCATGG
GACCCGGTGATTGCCGTCGGCAACACCGCCCGCCAGCCAGCCACGGTTGCCATCGCGGCCGGAAGGGCGTTTACCTTCGG
CTACGCCGAACACGCCGAGATGTTGCGCGCCGCCGGGGCTGAAGTGGTCGAGTTCGACCCGCTCAGCGAAACTCTGCCCG
AGGGTACGGACGCGGTGGTGTTGCCCGGCGGATTCCCCGAGCAGTTCACCGCCGAGTTGTCCGCCAACGACACCGTCCGG
CGGCAGATCAACGAACTGGCCGCTGCCGGCGCCCCGGTGCATGCCGAATGTGCCGGCCTGCTCTATCTGGTTTCTGAACT
CGACGGACACCCGATGTGCGGTGTGGTGGCCGGATCGGCGCGGTTCACCCAGCATCTCAAGCTGGGTTATCGCGACGCCG
TCGCGGTTGTTGATTCGGCGCTGTACTCCGTCGGCGAGCGCGTGGTTGGACATGAATTCCACCGAACCGCAGTCACATTC
GCCGATAGCTATCAGCCCGCGTGGGTGTACCAGGGCCAAGACGTGGACGACGTGCGAGACGGCGCGGTGCACAGCGGCGT
GCACGCGTCCTACCTGCATACCCATCCGGCCGCAACACCCGGTGCGGTGGCGCGTTTCGTCGCACATGCGGCCTGCAATA
CTCCACGAGCGTAA

Upstream 100 bases:

>100_bases
CCCAGCGGTTGGTTGCGGCCGCCGATCTGGTGACCGAGATGACCAAGGTGAAGCACCCGATGGATGCGGGCCGCAAGGGG
CAGAAGGGCATCGAGTGGTG

Downstream 100 bases:

>100_bases
CGGCACTGCGAAAAGCCGGGCCGGAAATTCGCAGTGGCGTTACATTCGCGGCCAAAGGGGTGGCGACCCGCGGCGCCCGG
TCCCCGCGCTTGCGATCGCC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRVSAVAVAAPASGSGKTTIATGLIGALRQAGHTVAPFKVGPDFIDPGYHALAAGRPGRNLDPVLVGERLIGPLYAHGVA
GADIAVIEGVLGLFDGRIGPAGGAPAAGSTAHVAALLGAPVILVVDARGQSHSVAALLHGFSTFDTATRIAGVILNRVGS
ARHEQVLRQACDQAGVAVLGAIPRTAELELPTRYLGLVTAVEYGRRARLAVQAMTAVVARHVDLAAVIACAGSQAAHPPW
DPVIAVGNTARQPATVAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVVLPGGFPEQFTAELSANDTVR
RQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVVAGSARFTQHLKLGYRDAVAVVDSALYSVGERVVGHEFHRTAVTF
ADSYQPAWVYQGQDVDDVRDGAVHSGVHASYLHTHPAATPGAVARFVAHAACNTPRA

Sequences:

>Translated_457_residues
MRVSAVAVAAPASGSGKTTIATGLIGALRQAGHTVAPFKVGPDFIDPGYHALAAGRPGRNLDPVLVGERLIGPLYAHGVA
GADIAVIEGVLGLFDGRIGPAGGAPAAGSTAHVAALLGAPVILVVDARGQSHSVAALLHGFSTFDTATRIAGVILNRVGS
ARHEQVLRQACDQAGVAVLGAIPRTAELELPTRYLGLVTAVEYGRRARLAVQAMTAVVARHVDLAAVIACAGSQAAHPPW
DPVIAVGNTARQPATVAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVVLPGGFPEQFTAELSANDTVR
RQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVVAGSARFTQHLKLGYRDAVAVVDSALYSVGERVVGHEFHRTAVTF
ADSYQPAWVYQGQDVDDVRDGAVHSGVHASYLHTHPAATPGAVARFVAHAACNTPRA
>Mature_457_residues
MRVSAVAVAAPASGSGKTTIATGLIGALRQAGHTVAPFKVGPDFIDPGYHALAAGRPGRNLDPVLVGERLIGPLYAHGVA
GADIAVIEGVLGLFDGRIGPAGGAPAAGSTAHVAALLGAPVILVVDARGQSHSVAALLHGFSTFDTATRIAGVILNRVGS
ARHEQVLRQACDQAGVAVLGAIPRTAELELPTRYLGLVTAVEYGRRARLAVQAMTAVVARHVDLAAVIACAGSQAAHPPW
DPVIAVGNTARQPATVAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVVLPGGFPEQFTAELSANDTVR
RQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVVAGSARFTQHLKLGYRDAVAVVDSALYSVGERVVGHEFHRTAVTF
ADSYQPAWVYQGQDVDDVRDGAVHSGVHASYLHTHPAATPGAVARFVAHAACNTPRA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBB_MYCBO (P63836)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856518.1
- ProteinModelPortal:   P63836
- EnsemblBacteria:   EBMYCT00000014978
- GeneID:   1092236
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2873c
- GeneTree:   EBGT00050000017692
- HOGENOM:   HBG634910
- OMA:   HHTAICG
- ProtClustDB:   PRK01077
- BioCyc:   MBOV233413:MB2873C-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.9

Molecular weight: Translated: 46959; Mature: 46959

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 337-337 ACT_SITE 433-433

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSAVAVAAPASGSGKTTIATGLIGALRQAGHTVAPFKVGPDFIDPGYHALAAGRPGRN
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECCCCCCC
LDPVLVGERLIGPLYAHGVAGADIAVIEGVLGLFDGRIGPAGGAPAAGSTAHVAALLGAP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
VILVVDARGQSHSVAALLHGFSTFDTATRIAGVILNRVGSARHEQVLRQACDQAGVAVLG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEE
AIPRTAELELPTRYLGLVTAVEYGRRARLAVQAMTAVVARHVDLAAVIACAGSQAAHPPW
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DPVIAVGNTARQPATVAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVV
CCEEECCCCCCCCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEECCCHHHCCCCCCEEE
LPGGFPEQFTAELSANDTVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVVAGSA
ECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCH
RFTQHLKLGYRDAVAVVDSALYSVGERVVGHEFHRTAVTFADSYQPAWVYQGQDVDDVRD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHHHC
GAVHSGVHASYLHTHPAATPGAVARFVAHAACNTPRA
CHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRVSAVAVAAPASGSGKTTIATGLIGALRQAGHTVAPFKVGPDFIDPGYHALAAGRPGRN
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEEECCCCCCC
LDPVLVGERLIGPLYAHGVAGADIAVIEGVLGLFDGRIGPAGGAPAAGSTAHVAALLGAP
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
VILVVDARGQSHSVAALLHGFSTFDTATRIAGVILNRVGSARHEQVLRQACDQAGVAVLG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEE
AIPRTAELELPTRYLGLVTAVEYGRRARLAVQAMTAVVARHVDLAAVIACAGSQAAHPPW
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DPVIAVGNTARQPATVAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVV
CCEEECCCCCCCCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEECCCHHHCCCCCCEEE
LPGGFPEQFTAELSANDTVRRQINELAAAGAPVHAECAGLLYLVSELDGHPMCGVVAGSA
ECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCH
RFTQHLKLGYRDAVAVVDSALYSVGERVVGHEFHRTAVTFADSYQPAWVYQGQDVDDVRD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHHHC
GAVHSGVHASYLHTHPAATPGAVARFVAHAACNTPRA
CHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972