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The map label for this gene is cysG [H]

Identifier: 148824037

GI number: 148824037

Start: 3166292

End: 3167509

Strand: Reverse

Name: cysG [H]

Synonym: TBFG_12862

Alternate gene names: 148824037

Gene position: 3167509-3166292 (Counterclockwise)

Preceding gene: 148824038

Following gene: 148824036

Centisome position: 71.59

GC content: 66.26

Gene sequence:

>1218_bases
GTGACCGAGAACCCCTATCTGGTCGGGTTACGGCTGGCTGGCAAGAAGGTCGTCGTGGTTGGCGGGGGCACGGTCGCCCA
GCGCCGGTTACCCCTGCTGATCGCCAGTGGCGCGGACGTGCACGTGATCGCCCCCAGCGTCACCCCCGCCGTCGAGGCGA
TGGACCAGATCACCTTGTCGGTGCGTGACTACCGCGACGGCGACCTTGACGGCGCCTGGTATGCGATCGCGGCCACCGAT
GACGCGCGGGTGAACGTGGCTGTCGTCGCCGAGGCGGAGCGCCGACGGATCTTTTGCGTCCGGGCCGATATCGCGGTGGA
GGGGACGGCGGTGACCCCGGCGTCATTCAGCTATGCGGGCCTGTCGGTGGGGGTGCTCGCCGGTGGTGAGCACCGCCGTT
CGGCGGCGATCCGCTCGGCAATCCGGGAGGCGTTGCAGCAGGGCGTCATCACTGCGCAGAGTTCCGACGTCCTCAGCGGC
GGAGTGGCGTTGGTCGGCGGCGGTCCCGGCGATCCCGAACTGATCACGGTTCGCGGTCGCCGGCTGCTTGCCCAGGCCGA
TGTCGTGGTCGCCGACCGGCTCGCCCCGCCCGAACTGCTGGCCGAGCTGCCGCCGCACGTAGAAGTCATCGACGCGGCCA
AGATCCCTTACGGCCGGGCCATGGCCCAGGACGCGATCAACGCTGTCCTGATCGAACGGGCCAGATCCGGCAACTTTGTG
GTCCGTCTCAAAGGGGGCGACCCCTTCGTGTTCGCCCGGGGCTATGAAGAAGTGCTGGCATGTGCCCACGCCGGAATCCC
GGTCACCGTGGTGCCAGGTGTGACGAGTGCCATAGCCGTGCCCGCTATGGCGGGCGTTCCAGTCACTCACCGGGCCATGA
CCCACGAATTCGTGGTGGTCAGTGGCCATCTTGCGCCCGGTCATCCCGAATCGTTAGTGAATTGGGATGCATTGGCTGCA
TTGACGGGCACCATCGTTTTGCTGATGGCGGTCGAACGCATCGAGCTTTTCGTTGACGTTCTGCTAAAGGGTGGCCGAAC
TGCGGATACGCCGGTACTGGTGGTTCAACACGGAACGACCGCCGCTCAACAGACGTTGCGGGCCACCCTTGCCGACACGC
CGGAGAAGGTCCGCGCGGCGGGGATCCGACCTCCCGCGATCATCGTGATCGGGGCTGTAGTCGGCCTGAGCGGCGTTCGG
GGTTTAAACAATTCTTAA

Upstream 100 bases:

>100_bases
CGCGGCGCCCGGTCCCCGCGCTTGCGATCGCCACTGGCCCTGATGGTGGCGACCCGCGGCGCCCGGTCCCCGCGCTTGCG
ATCGCCACTAGGCTTGGCGG

Downstream 100 bases:

>100_bases
GAATACTGTAAGGTAACCCGCTATGACGGCTCTCAACGACACAGAGCGGGCGGTCCGTAACTGGACAGCCGGACGCCCAC
ACCGTCCGGCCCCGATGCGC

Product: multi-functional enzyme siroheme synthase cysG: uroporphyrin-III C-methyltransferase + precorrin-2 oxidase + ferrochelatase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLSVRDYRDGDLDGAWYAIAATD
DARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAGLSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSG
GVALVGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFV
VRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVVSGHLAPGHPESLVNWDALAA
LTGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVR
GLNNS

Sequences:

>Translated_405_residues
MTENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLSVRDYRDGDLDGAWYAIAATD
DARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAGLSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSG
GVALVGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFV
VRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVVSGHLAPGHPESLVNWDALAA
LTGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVR
GLNNS
>Mature_404_residues
TENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLSVRDYRDGDLDGAWYAIAATDD
ARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAGLSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSGG
VALVGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFVV
RLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVVSGHLAPGHPESLVNWDALAAL
TGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVRG
LNNS

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=454, Percent_Identity=31.057268722467, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322922, Length=265, Percent_Identity=33.2075471698113, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 41971; Mature: 41840

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLS
CCCCCEEEEEEECCCEEEEECCCCCHHCCCCEEEECCCCEEEECCCCCHHHHHHHHEEEE
VRDYRDGDLDGAWYAIAATDDARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAG
EECCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCEEEEEEECEEEECCEECCCCCCCCC
LSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSGGVALVGGGPGDPELITVRGR
EEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCEEEECCC
RLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFV
EEEECCCEEEECCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVV
EEEECCCCEEEECCHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHCCEEEEEE
SGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTT
ECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCH
AAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVRGLNNS
HHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCCCCC
>Mature Secondary Structure 
TENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLS
CCCCEEEEEEECCCEEEEECCCCCHHCCCCEEEECCCCEEEECCCCCHHHHHHHHEEEE
VRDYRDGDLDGAWYAIAATDDARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAG
EECCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCEEEEEEECEEEECCEECCCCCCCCC
LSVGVLAGGEHRRSAAIRSAIREALQQGVITAQSSDVLSGGVALVGGGPGDPELITVRGR
EEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCCCCCCEEEECCC
RLLAQADVVVADRLAPPELLAELPPHVEVIDAAKIPYGRAMAQDAINAVLIERARSGNFV
EEEECCCEEEECCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVPAMAGVPVTHRAMTHEFVVV
EEEECCCCEEEECCHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHCCEEEEEE
SGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGGRTADTPVLVVQHGTT
ECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCH
AAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVRGLNNS
HHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA