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The map label for this gene is proS [H]

Identifier: 148824035

GI number: 148824035

Start: 3162840

End: 3164588

Strand: Reverse

Name: proS [H]

Synonym: TBFG_12860

Alternate gene names: 148824035

Gene position: 3164588-3162840 (Counterclockwise)

Preceding gene: 148824036

Following gene: 148824032

Centisome position: 71.53

GC content: 65.87

Gene sequence:

>1749_bases
GTGATCACCCGGATGTCCGAGTTGTTCTTGCGCACCCTGCGCGACGATCCCGCCGACGCCGAAGTGGCCAGCCACAAACT
GCTGATCCGGGCCGGCTACATCCGGCCCGTCGCGCCCGGGCTGTACAGCTGGTTACCGCTCGGCCTGCGAGTGCTGCGCA
ACATAGAACGGGTCATCCGCGACGAGATGAACGCCATCGGTGGACAGGAGATCCTATTTCCTGCCCTGCTGCCGCGGGCA
CCGTACGAGACGACCAACCGGTGGACCCAATACGGCGACAGCGTGTTTCGGCTCAAGGACCGCCGCGGCAACGACTACCT
GCTGGGCCCCACCCACGAAGAGCTGTTCACCCTGACCGTGAAGGGCGAGTACAGCTCCTACAAGGACTTCCCGCTCACGC
TGTACCAAATCCAGACCAAGTACCGCGACGAGGCGCGGCCGCGGGCCGGCATCCTGCGCGCTCGGGAGTTCGTCATGAAG
GACTCCTACTCCTTTGACATCGACGCCGCCGGGCTGAAGGCGGCCTACCACGCTCACCGGGAGGCCTACCAGCGCATATT
TGACCGACTTCAGGTGCGCTATGTCATCGTTTCGGCAGTGTCCGGGGCGATGGGCGGCAGTGCGTCCGAAGAGTTCTTGG
CTGAGAGTCCGTCCGGGGAGGACGCATTTGTCCGGTGCCTCGAGTCGGGTTATGCGGCCAACGTCGAGGCGGTTGTCACC
GCCCGCCCGGACACCCTGCCCATCGATGGGCTGCCCGAGGCGGTGGTCCACGACACCGGCGACACCCCAACCATCGCCAG
CCTGGTGGCCTGGGCCAACGAGGCCGATCTCGGCCGCACGGTGACCGCCGCGGACACCCTGAAGAACGTCTTAATCAAAG
TCCGACAGCCGGGAGGGGACACCGAACTGCTGGCAATCGGCGTGCCCGGCGACCGTGAGGTCGATGACAAAAGGTTGGGC
GCAGCGCTGGAACCGGCTGACTACGCGTTGCTCGACGACGATGATTTCGCCAAGCACCCGTTCCTGGTTAAGGGTTATAT
CGGGCCAAAGGCGTTGCGGGAGAACAATGTCCGCTATCTCGTCGACCCGCGGATAGTCGACGGCACCAGCTGGATCACCG
GGGCGGACCAACCCGGCCGCCATGTTGTCGGTTTGGTGGCCGGCCGCGACTTCACCGCAGACGGCACCATTGAGGCCGCC
GAGGTGCGCGAAGGCGATCCATCTCCCGACGGCGCCGGTCCGCTGGTCATGGCGCGCGGTATCGAAATTGGGCACATCTT
CCAGCTGGGGAGCAAATACACCGACGCCTTCACCGCCGACGTGCTCGGCGAGGATGGCAAGCCTGTGCGGTTGACCATGG
GTTCCTACGGCATCGGTGTGTCTCGGTTGGTTGCCGTCGTTGCCGAGCAGCACCACGACGAGCTGGGCCTTCGCTGGCCG
TCGACGGTCGCGCCGTTCGATGTTCACCTGGTGATCGCGAACAAGGACGCGCAGGCTCGCGCCGGGGCGACCGCGCTGGC
CGCCGATCTGGATCGGCTGGGGGTTGAGGTGTTGCTGGACGACCGCCAGGCATCGCCCGGCGTCAAGTTCAAAGATGCCG
AGCTGTTGGGTATGCCCTGGATCGTTGTCGTGGGGCGCGGCTGGGCGGACGGCGTGGTCGAGCTGCGCGACCGGTTCAGC
GGTCAGACTCGCGAGCTGGTTGCCGGCGCCTCGCTGGCCACCGATATCGCAGCGGCCGTCACCGGGTAG

Upstream 100 bases:

>100_bases
GGCGAGCTGTAACCCTTCCCGCCGAGCAGTCATAAAAGCTCCCCTTTTCGGGCAGACCTGGGCGCTTTTGCGTCTGCTCG
GCGAATTAGGCTTGCCGCCT

Downstream 100 bases:

>100_bases
CTATTCGTCCCCGCCCGGAAAGGCCGCGGTGATGGGCCAGGCGCCCAGCACCCTGTTCCAGCGGGTGGCCATCACCGCGC
TCTCGGTCAGAGCCGTCGAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MITRMSELFLRTLRDDPADAEVASHKLLIRAGYIRPVAPGLYSWLPLGLRVLRNIERVIRDEMNAIGGQEILFPALLPRA
PYETTNRWTQYGDSVFRLKDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPLTLYQIQTKYRDEARPRAGILRAREFVMK
DSYSFDIDAAGLKAAYHAHREAYQRIFDRLQVRYVIVSAVSGAMGGSASEEFLAESPSGEDAFVRCLESGYAANVEAVVT
ARPDTLPIDGLPEAVVHDTGDTPTIASLVAWANEADLGRTVTAADTLKNVLIKVRQPGGDTELLAIGVPGDREVDDKRLG
AALEPADYALLDDDDFAKHPFLVKGYIGPKALRENNVRYLVDPRIVDGTSWITGADQPGRHVVGLVAGRDFTADGTIEAA
EVREGDPSPDGAGPLVMARGIEIGHIFQLGSKYTDAFTADVLGEDGKPVRLTMGSYGIGVSRLVAVVAEQHHDELGLRWP
STVAPFDVHLVIANKDAQARAGATALAADLDRLGVEVLLDDRQASPGVKFKDAELLGMPWIVVVGRGWADGVVELRDRFS
GQTRELVAGASLATDIAAAVTG

Sequences:

>Translated_582_residues
MITRMSELFLRTLRDDPADAEVASHKLLIRAGYIRPVAPGLYSWLPLGLRVLRNIERVIRDEMNAIGGQEILFPALLPRA
PYETTNRWTQYGDSVFRLKDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPLTLYQIQTKYRDEARPRAGILRAREFVMK
DSYSFDIDAAGLKAAYHAHREAYQRIFDRLQVRYVIVSAVSGAMGGSASEEFLAESPSGEDAFVRCLESGYAANVEAVVT
ARPDTLPIDGLPEAVVHDTGDTPTIASLVAWANEADLGRTVTAADTLKNVLIKVRQPGGDTELLAIGVPGDREVDDKRLG
AALEPADYALLDDDDFAKHPFLVKGYIGPKALRENNVRYLVDPRIVDGTSWITGADQPGRHVVGLVAGRDFTADGTIEAA
EVREGDPSPDGAGPLVMARGIEIGHIFQLGSKYTDAFTADVLGEDGKPVRLTMGSYGIGVSRLVAVVAEQHHDELGLRWP
STVAPFDVHLVIANKDAQARAGATALAADLDRLGVEVLLDDRQASPGVKFKDAELLGMPWIVVVGRGWADGVVELRDRFS
GQTRELVAGASLATDIAAAVTG
>Mature_582_residues
MITRMSELFLRTLRDDPADAEVASHKLLIRAGYIRPVAPGLYSWLPLGLRVLRNIERVIRDEMNAIGGQEILFPALLPRA
PYETTNRWTQYGDSVFRLKDRRGNDYLLGPTHEELFTLTVKGEYSSYKDFPLTLYQIQTKYRDEARPRAGILRAREFVMK
DSYSFDIDAAGLKAAYHAHREAYQRIFDRLQVRYVIVSAVSGAMGGSASEEFLAESPSGEDAFVRCLESGYAANVEAVVT
ARPDTLPIDGLPEAVVHDTGDTPTIASLVAWANEADLGRTVTAADTLKNVLIKVRQPGGDTELLAIGVPGDREVDDKRLG
AALEPADYALLDDDDFAKHPFLVKGYIGPKALRENNVRYLVDPRIVDGTSWITGADQPGRHVVGLVAGRDFTADGTIEAA
EVREGDPSPDGAGPLVMARGIEIGHIFQLGSKYTDAFTADVLGEDGKPVRLTMGSYGIGVSRLVAVVAEQHHDELGLRWP
STVAPFDVHLVIANKDAQARAGATALAADLDRLGVEVLLDDRQASPGVKFKDAELLGMPWIVVVGRGWADGVVELRDRFS
GQTRELVAGASLATDIAAAVTG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=40.7894736842105, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1786392, Length=579, Percent_Identity=40.4145077720207, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=38.4259259259259, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=567, Percent_Identity=28.9241622574956, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24656200, Length=216, Percent_Identity=37.5, Blast_Score=159, Evalue=6e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63302; Mature: 63302

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRMSELFLRTLRDDPADAEVASHKLLIRAGYIRPVAPGLYSWLPLGLRVLRNIERVIR
CCCCHHHHHHHHHCCCCCCHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DEMNAIGGQEILFPALLPRAPYETTNRWTQYGDSVFRLKDRRGNDYLLGPTHEELFTLTV
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEE
KGEYSSYKDFPLTLYQIQTKYRDEARPRAGILRAREFVMKDSYSFDIDAAGLKAAYHAHR
ECCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EAYQRIFDRLQVRYVIVSAVSGAMGGSASEEFLAESPSGEDAFVRCLESGYAANVEAVVT
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEE
ARPDTLPIDGLPEAVVHDTGDTPTIASLVAWANEADLGRTVTAADTLKNVLIKVRQPGGD
ECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEECCCCC
TELLAIGVPGDREVDDKRLGAALEPADYALLDDDDFAKHPFLVKGYIGPKALRENNVRYL
CEEEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHCCCCEEE
VDPRIVDGTSWITGADQPGRHVVGLVAGRDFTADGTIEAAEVREGDPSPDGAGPLVMARG
ECCEEECCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEHHCCCCCCCCCCCCCEEEECC
IEIGHIFQLGSKYTDAFTADVLGEDGKPVRLTMGSYGIGVSRLVAVVAEQHHDELGLRWP
CCEEHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
STVAPFDVHLVIANKDAQARAGATALAADLDRLGVEVLLDDRQASPGVKFKDAELLGMPW
CCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCEECCCE
IVVVGRGWADGVVELRDRFSGQTRELVAGASLATDIAAAVTG
EEEEECCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MITRMSELFLRTLRDDPADAEVASHKLLIRAGYIRPVAPGLYSWLPLGLRVLRNIERVIR
CCCCHHHHHHHHHCCCCCCHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
DEMNAIGGQEILFPALLPRAPYETTNRWTQYGDSVFRLKDRRGNDYLLGPTHEELFTLTV
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEEEEE
KGEYSSYKDFPLTLYQIQTKYRDEARPRAGILRAREFVMKDSYSFDIDAAGLKAAYHAHR
ECCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EAYQRIFDRLQVRYVIVSAVSGAMGGSASEEFLAESPSGEDAFVRCLESGYAANVEAVVT
HHHHHHHHHHHHHHHEEEHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEE
ARPDTLPIDGLPEAVVHDTGDTPTIASLVAWANEADLGRTVTAADTLKNVLIKVRQPGGD
ECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEECCCCC
TELLAIGVPGDREVDDKRLGAALEPADYALLDDDDFAKHPFLVKGYIGPKALRENNVRYL
CEEEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHCCCCEEE
VDPRIVDGTSWITGADQPGRHVVGLVAGRDFTADGTIEAAEVREGDPSPDGAGPLVMARG
ECCEEECCCCEECCCCCCCCEEEEEEECCCCCCCCCEEEHHCCCCCCCCCCCCCEEEECC
IEIGHIFQLGSKYTDAFTADVLGEDGKPVRLTMGSYGIGVSRLVAVVAEQHHDELGLRWP
CCEEHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
STVAPFDVHLVIANKDAQARAGATALAADLDRLGVEVLLDDRQASPGVKFKDAELLGMPW
CCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCEECCCE
IVVVGRGWADGVVELRDRFSGQTRELVAGASLATDIAAAVTG
EEEEECCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]