Click here to switch to the map view.

The map label for this gene is efpA

Identifier: 148824036

GI number: 148824036

Start: 3164677

End: 3166269

Strand: Reverse

Name: efpA

Synonym: TBFG_12861

Alternate gene names: 148824036

Gene position: 3166269-3164677 (Counterclockwise)

Preceding gene: 148824037

Following gene: 148824035

Centisome position: 71.56

GC content: 64.91

Gene sequence:

>1593_bases
ATGACGGCTCTCAACGACACAGAGCGGGCGGTCCGTAACTGGACAGCCGGACGCCCACACCGTCCGGCCCCGATGCGCCC
GCCGCGCTCGGAGGAGACCGCTTCAGAGCGCCCCAGCAGGTACTACCCGACTTGGCTGCCCTCGCGCAGCTTTATCGCTG
CGGTTATTGCTATCGGCGGGATGCAGCTGCTGGCGACCATGGACAGCACCGTCGCCATCGTCGCGCTACCTAAGATTCAA
AACGAGCTGAGCTTGTCTGATGCCGGCCGCAGCTGGGTGATCACCGCCTACGTGCTGACCTTCGGCGGGCTGATGCTGCT
CGGCGGCCGGCTTGGCGACACCATCGGGCGCAAACGCACCTTCATTGTTGGCGTTGCGCTATTCACCATCTCGTCGGTGC
TGTGCGCGGTCGCCTGGGACGAGGCGACGTTGGTGATCGCCCGGTTGTCCCAGGGTGTGGGGTCGGCCATCGCATCTCCG
ACCGGTCTGGCGCTGGTGGCGACCACGTTCCCCAAGGGACCTGCCCGCAACGCCGCGACGGCGGTGTTCGCCGCGATGAC
CGCGATCGGGTCGGTGATGGGGCTGGTGGTCGGCGGAGCACTGACCGAGGTGTCATGGCGGTGGGCGTTCCTGGTGAACG
TGCCGATCGGGCTGGTGATGATCTACCTGGCCCGCACCGCCCTACGGGAAACCAACAAAGAACGGATGAAGCTCGACGCC
ACCGGGGCCATACTGGCCACGCTGGCATGCACCGCGGCGGTTTTCGCCTTCTCGATCGGTCCTGAAAAGGGCTGGATGTC
AGGCATTACCATCGGTTCGGGCCTGGTGGCCTTGGCGGCCGCTGTCGCGTTTGTCATCGTGGAGCGCACTGCCGAGAACC
CCGTCGTGCCGTTCCACTTGTTCCGCGACCGCAACCGGTTGGTCACGTTCAGCGCGATCCTGTTGGCCGGCGGCGTCATG
TTCAGCCTGACCGTCTGCATCGGCCTGTACGTGCAGGACATCTTGGGCTACAGCGCGCTACGCGCGGGCGTAGGTTTCAT
CCCGTTCGTCATCGCGATGGGAATCGGCCTAGGTGTGTCCTCGCAGCTGGTGTCCCGGTTTTCGCCACGGGTGTTGACCA
TCGGCGGCGGATATCTGCTATTCGGCGCCATGCTGTACGGCTCATTTTTCATGCACCGTGGTGTGCCCTACTTCCCCAAC
CTGGTCATGCCGATCGTCGTCGGCGGGATTGGCATCGGCATGGCCGTCGTCCCGCTGACTCTGTCGGCGATCGCTGGCGT
CGGCTTCGACCAGATCGGTCCGGTATCGGCAATTGCGCTGATGCTGCAGAGCCTGGGCGGTCCGCTGGTGCTCGCCGTCA
TCCAGGCTGTGATCACGTCGCGCACGCTGTACCTGGGCGGTACCACCGGTCCGGTGAAGTTCATGAACGACGTGCAGTTG
GCCGCGCTTGACCACGCCTACACCTACGGCCTGCTGTGGGTGGCCGGAGCGGCCATCATCGTCGGCGGTATGGCGCTGTT
TATCGGGTATACGCCGCAGCAGGTTGCCCATGCGCAGGAGGTCAAGGAAGCGATCGACGCCGGCGAGCTGTAA

Upstream 100 bases:

>100_bases
GGGATCCGACCTCCCGCGATCATCGTGATCGGGGCTGTAGTCGGCCTGAGCGGCGTTCGGGGTTTAAACAATTCTTAAGA
ATACTGTAAGGTAACCCGCT

Downstream 100 bases:

>100_bases
CCCTTCCCGCCGAGCAGTCATAAAAGCTCCCCTTTTCGGGCAGACCTGGGCGCTTTTGCGTCTGCTCGGCGAATTAGGCT
TGCCGCCTGTGATCACCCGG

Product: integral membrane efflux protein efpA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTALNDTERAVRNWTAGRPHRPAPMRPPRSEETASERPSRYYPTWLPSRSFIAAVIAIGGMQLLATMDSTVAIVALPKIQ
NELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATLVIARLSQGVGSAIASP
TGLALVATTFPKGPARNAATAVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPIGLVMIYLARTALRETNKERMKLDA
TGAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPFHLFRDRNRLVTFSAILLAGGVM
FSLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPN
LVMPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMNDVQL
AALDHAYTYGLLWVAGAAIIVGGMALFIGYTPQQVAHAQEVKEAIDAGEL

Sequences:

>Translated_530_residues
MTALNDTERAVRNWTAGRPHRPAPMRPPRSEETASERPSRYYPTWLPSRSFIAAVIAIGGMQLLATMDSTVAIVALPKIQ
NELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATLVIARLSQGVGSAIASP
TGLALVATTFPKGPARNAATAVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPIGLVMIYLARTALRETNKERMKLDA
TGAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPFHLFRDRNRLVTFSAILLAGGVM
FSLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPN
LVMPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMNDVQL
AALDHAYTYGLLWVAGAAIIVGGMALFIGYTPQQVAHAQEVKEAIDAGEL
>Mature_529_residues
TALNDTERAVRNWTAGRPHRPAPMRPPRSEETASERPSRYYPTWLPSRSFIAAVIAIGGMQLLATMDSTVAIVALPKIQN
ELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEATLVIARLSQGVGSAIASPT
GLALVATTFPKGPARNAATAVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPIGLVMIYLARTALRETNKERMKLDAT
GAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPFHLFRDRNRLVTFSAILLAGGVMF
SLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPNL
VMPIVVGGIGIGMAVVPLTLSAIAGVGFDQIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMNDVQLA
ALDHAYTYGLLWVAGAAIIVGGMALFIGYTPQQVAHAQEVKEAIDAGEL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=26.3546798029557, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789042, Length=420, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=27.0935960591133, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=24.1895261845387, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=318, Percent_Identity=29.2452830188679, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081723, Length=157, Percent_Identity=35.031847133758, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790146, Length=318, Percent_Identity=25.1572327044025, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=366, Percent_Identity=24.3169398907104, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=308, Percent_Identity=25.6493506493506, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323935, Length=460, Percent_Identity=25, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=312, Percent_Identity=24.6794871794872, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=35.2517985611511, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=310, Percent_Identity=24.8387096774194, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=34.8148148148148, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EFPA_MYCTU (O05813)

Other databases:

- EMBL:   L39922
- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   A70589
- RefSeq:   NP_217362.1
- RefSeq:   NP_337424.1
- ProteinModelPortal:   O05813
- EnsemblBacteria:   EBMYCT00000001073
- EnsemblBacteria:   EBMYCT00000071483
- GeneID:   888575
- GeneID:   925363
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2912
- KEGG:   mtu:Rv2846c
- TIGR:   MT2912
- TubercuList:   Rv2846c
- GeneTree:   EBGT00050000014781
- HOGENOM:   HBG679341
- OMA:   TRETETA
- ProtClustDB:   CLSK792121
- GO:   GO:0040007
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 55580; Mature: 55449

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c6254f8)-; HASH(0x1cb1bc9c)-; HASH(0x1c1168b0)-; HASH(0x1ccfe794)-; HASH(0x1ce10f10)-; HASH(0x1cc67ac0)-; HASH(0x1c59ca20)-; HASH(0x1ac352a8)-; HASH(0x1c5357d8)-; HASH(0x1c94aee8)-; HASH(0x1ce96610)-; HASH(0x1cd9f74c)-; HASH(0x1c7f7600)-; HASH(0x1cd9696c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNDTERAVRNWTAGRPHRPAPMRPPRSEETASERPSRYYPTWLPSRSFIAAVIAIGG
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MQLLATMDSTVAIVALPKIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRT
HHHHHHCCCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHH
FIVGVALFTISSVLCAVAWDEATLVIARLSQGVGSAIASPTGLALVATTFPKGPARNAAT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
AVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPIGLVMIYLARTALRETNKERMKLDA
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCHHHEEECH
TGAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPFHL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEHE
FRDRNRLVTFSAILLAGGVMFSLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVS
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCH
SQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPNLVMPIVVGGIGIGMAVVPLT
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSAIAGVGFDQIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMNDVQL
HHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHCCHHH
AALDHAYTYGLLWVAGAAIIVGGMALFIGYTPQQVAHAQEVKEAIDAGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
TALNDTERAVRNWTAGRPHRPAPMRPPRSEETASERPSRYYPTWLPSRSFIAAVIAIGG
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MQLLATMDSTVAIVALPKIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRT
HHHHHHCCCCEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHH
FIVGVALFTISSVLCAVAWDEATLVIARLSQGVGSAIASPTGLALVATTFPKGPARNAAT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
AVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPIGLVMIYLARTALRETNKERMKLDA
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCHHHEEECH
TGAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPFHL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEHE
FRDRNRLVTFSAILLAGGVMFSLTVCIGLYVQDILGYSALRAGVGFIPFVIAMGIGLGVS
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCH
SQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPNLVMPIVVGGIGIGMAVVPLT
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSAIAGVGFDQIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMNDVQL
HHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHCCHHH
AALDHAYTYGLLWVAGAAIIVGGMALFIGYTPQQVAHAQEVKEAIDAGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036