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The map label for this gene is pqqL [C]

Identifier: 148823970

GI number: 148823970

Start: 3101000

End: 3102316

Strand: Reverse

Name: pqqL [C]

Synonym: TBFG_12795

Alternate gene names: 148823970

Gene position: 3102316-3101000 (Counterclockwise)

Preceding gene: 148823971

Following gene: 148823969

Centisome position: 70.12

GC content: 68.56

Gene sequence:

>1317_bases
ATGCCGCGACGGTCACCAGCTGACCCCGCGGCGGCGCTGGCGCCGCGGCGCACCACCCTGCCGGGCGGGCTGCGAGTGGT
CACCGAATTCCTGCCCGCGGTGCACTCCGCGTCGGTCGGGGTGTGGGTCGGCGTCGGATCGCGCGACGAAGGCGCCACGG
TGGCCGGGGCGGCGCACTTCCTTGAGCATTTGCTGTTCAAGTCGACGCCCACCCGCTCTGCCGTGGACATTGCGCAGGCG
ATGGACGCGGTGGGCGGGGAACTGAACGCATTCACCGCCAAGGAGCACACCTGCTACTACGCCCACGTGCTCGGCAGCGA
CTTGCCGTTGGCCGTCGACCTGGTCGCCGATGTGGTGCTCAACGGCCGCTGTGCCGCCGACGATGTCGAGGTGGAACGTG
ACGTCGTCCTCGAGGAGATCGCGATGCGCGACGACGACCCCGAGGACGCCTTGGCGGACATGTTCCTGGCGGCGTTGTTC
GGCGACCACCCGGTCGGTCGCCCGGTGATCGGCAGCGCGCAATCCGTGTCGGTGATGACGCGGGCTCAACTGCAATCGTT
TCACCTGCGGCGCTATACCCCGGAGCGGATGGTCGTCGCGGCCGCCGGCAATGTGGATCACGACGGGCTGGTTGCGTTGG
TCCGCGAGCACTTCGGGTCCCGGTTGGTCCGGGGGAGACGGCCAGTTGCGCCGCGCAAGGGTACCGGCCGGGTCAACGGC
AGCCCCCGGTTGACACTGGTTAGCCGCGACGCCGAACAGACGCATGTGTCGCTGGGCATCCGCACACCCGGGCGCGGCTG
GGAGCATCGTTGGGCACTGTCGGTGCTGCACACCGCGCTGGGCGGTGGCTTGAGTTCCCGGCTGTTCCAGGAGGTCCGCG
AGACCCGCGGGCTGGCCTACTCGGTCTACTCCGCGCTGGATCTCTTCGCCGACAGCGGCGCGCTTTCGGTGTACGCGGCC
TGCCTGCCCGAACGCTTCGCCGACGTGATGCGGGTGACCGCCGATGTGCTGGAAAGCGTGGCACGCGACGGCATCACCGA
GGCGGAATGCGGCATCGCCAAGGGATCGCTGCGGGGTGGGCTGGTGCTAGGGCTGGAGGATTCCAGCTCCCGGATGAGCC
GGCTCGGCCGCAGCGAGTTGAACTACGGCAAGCACCGCAGCATCGAACACACCTTGCGGCAAATCGAGCAGGTCACCGTG
GAGGAGGTCAACGCGGTGGCCCGCCACCTGCTGAGCAGGCGCTACGGTGCTGCCGTTCTTGGCCCACACGGATCGAAACG
ATCACTGCCGCAACAACTTCGAGCGATGGTAGGGTAG

Upstream 100 bases:

>100_bases
GTCGGTGACAAGCTGCGGGTGGAGATCGCCGACATCGACAAACGGGGCAAGATCTCCCTGATCCTGGTCGCCGACGAGGA
CAGCACCGCCGCCGCTACCG

Downstream 100 bases:

>100_bases
CTCAAATGTCCGCTTCCTCACTCGCGCCGTGCCGGCGCTCGCCGTCGGCGAATTAGCCCGATGGTGTTGGGCTTCTGGGA
CATCGCGGTGCCGATCGTCG

Product: zinc protease pepR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPRRSPADPAAALAPRRTTLPGGLRVVTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHFLEHLLFKSTPTRSAVDIAQA
MDAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADVVLNGRCAADDVEVERDVVLEEIAMRDDDPEDALADMFLAALF
GDHPVGRPVIGSAQSVSVMTRAQLQSFHLRRYTPERMVVAAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNG
SPRLTLVSRDAEQTHVSLGIRTPGRGWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAYSVYSALDLFADSGALSVYAA
CLPERFADVMRVTADVLESVARDGITEAECGIAKGSLRGGLVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVTV
EEVNAVARHLLSRRYGAAVLGPHGSKRSLPQQLRAMVG

Sequences:

>Translated_438_residues
MPRRSPADPAAALAPRRTTLPGGLRVVTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHFLEHLLFKSTPTRSAVDIAQA
MDAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADVVLNGRCAADDVEVERDVVLEEIAMRDDDPEDALADMFLAALF
GDHPVGRPVIGSAQSVSVMTRAQLQSFHLRRYTPERMVVAAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNG
SPRLTLVSRDAEQTHVSLGIRTPGRGWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAYSVYSALDLFADSGALSVYAA
CLPERFADVMRVTADVLESVARDGITEAECGIAKGSLRGGLVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVTV
EEVNAVARHLLSRRYGAAVLGPHGSKRSLPQQLRAMVG
>Mature_437_residues
PRRSPADPAAALAPRRTTLPGGLRVVTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHFLEHLLFKSTPTRSAVDIAQAM
DAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADVVLNGRCAADDVEVERDVVLEEIAMRDDDPEDALADMFLAALFG
DHPVGRPVIGSAQSVSVMTRAQLQSFHLRRYTPERMVVAAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNGS
PRLTLVSRDAEQTHVSLGIRTPGRGWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAYSVYSALDLFADSGALSVYAAC
LPERFADVMRVTADVLESVARDGITEAECGIAKGSLRGGLVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVTVE
EVNAVARHLLSRRYGAAVLGPHGSKRSLPQQLRAMVG

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Homo sapiens, GI94538354, Length=437, Percent_Identity=30.6636155606407, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI46593007, Length=415, Percent_Identity=30.6024096385542, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI24308013, Length=449, Percent_Identity=28.0623608017817, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI50592988, Length=424, Percent_Identity=21.4622641509434, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1787770, Length=425, Percent_Identity=22.8235294117647, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71999683, Length=441, Percent_Identity=28.5714285714286, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17553678, Length=423, Percent_Identity=29.0780141843972, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323192, Length=423, Percent_Identity=30.4964539007092, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321813, Length=419, Percent_Identity=23.1503579952267, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319426, Length=236, Percent_Identity=23.3050847457627, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357875, Length=422, Percent_Identity=29.6208530805687, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24646943, Length=422, Percent_Identity=29.6208530805687, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI19921772, Length=428, Percent_Identity=28.0373831775701, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2782_MYCTU (P0A5S8)

Other databases:

- EMBL:   BX842580
- EMBL:   AE000516
- PIR:   E70883
- RefSeq:   NP_217298.1
- RefSeq:   NP_337357.1
- ProteinModelPortal:   P0A5S8
- SMR:   P0A5S8
- MEROPS:   M16.016
- EnsemblBacteria:   EBMYCT00000003639
- EnsemblBacteria:   EBMYCT00000069387
- GeneID:   888470
- GeneID:   925431
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2852
- KEGG:   mtu:Rv2782c
- TIGR:   MT2852
- TubercuList:   Rv2782c
- GeneTree:   EBGT00050000017176
- HOGENOM:   HBG476859
- OMA:   KHLASAT
- ProtClustDB:   CLSK792074
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C; SSF63411 Metalloenz_metal-bd

EC number: 3.4.99.- [C]

Molecular weight: Translated: 47073; Mature: 46942

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00143 INSULINASE

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRSPADPAAALAPRRTTLPGGLRVVTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHF
CCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LEHLLFKSTPTRSAVDIAQAMDAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADVVL
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
NGRCAADDVEVERDVVLEEIAMRDDDPEDALADMFLAALFGDHPVGRPVIGSAQSVSVMT
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RAQLQSFHLRRYTPERMVVAAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNG
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPRLTLVSRDAEQTHVSLGIRTPGRGWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAY
CCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
SVYSALDLFADSGALSVYAACLPERFADVMRVTADVLESVARDGITEAECGIAKGSLRGG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
LVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVTVEEVNAVARHLLSRRYGAAVL
EEEEECCCHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GPHGSKRSLPQQLRAMVG
CCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PRRSPADPAAALAPRRTTLPGGLRVVTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHF
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LEHLLFKSTPTRSAVDIAQAMDAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADVVL
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
NGRCAADDVEVERDVVLEEIAMRDDDPEDALADMFLAALFGDHPVGRPVIGSAQSVSVMT
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RAQLQSFHLRRYTPERMVVAAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNG
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPRLTLVSRDAEQTHVSLGIRTPGRGWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAY
CCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
SVYSALDLFADSGALSVYAACLPERFADVMRVTADVLESVARDGITEAECGIAKGSLRGG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
LVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVTVEEVNAVARHLLSRRYGAAVL
EEEEECCCHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GPHGSKRSLPQQLRAMVG
CCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036