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The map label for this gene is pnp [H]

Identifier: 148823971

GI number: 148823971

Start: 3102294

End: 3104552

Strand: Reverse

Name: pnp [H]

Synonym: TBFG_12796

Alternate gene names: 148823971

Gene position: 3104552-3102294 (Counterclockwise)

Preceding gene: 148823972

Following gene: 148823970

Centisome position: 70.17

GC content: 65.47

Gene sequence:

>2259_bases
ATGTCTGCCGCTGAAATTGACGAAGGCGTGTTCGAGACGACCGCCACCATCGACAACGGGAGCTTTGGCACCCGGACCAT
CCGCTTCGAGACCGGCCGATTGGCCTTGCAGGCCGCCGGCGCGGTGGTCGCCTACCTCGACGACGACAACATGCTGCTGT
CGGCGACCACCGCCAGCAAGAACCCCAAAGAACACTTCGACTTCTTCCCCCTCACGGTCGACGTCGAGGAGCGCATGTAT
GCGGCCGGCCGCATCCCCGGTTCGTTCTTCCGTCGCGAGGGCCGACCCTCCACCGACGCGATCCTGACCTGCCGGCTCAT
CGACCGCCCGCTGCGCCCGTCGTTTGTCGACGGGCTGCGCAACGAGATCCAAATCGTGGTGACGATTCTCAGCCTGGATC
CGGGCGATCTCTACGACGTATTGGCGATCAACGCGGCGTCGGCGTCCACCCAGCTGGGCGGTCTGCCGTTCTCCGGGCCC
ATCGGCGGTGTGCGGGTGGCGCTCATCGACGGCACCTGGGTCGGCTTCCCCACCGTCGACCAGATCGAGCGCGCCGTGTT
CGACATGGTCGTGGCCGGCCGGATCGTCGAGGGTGATGTTGCCATCATGATGGTCGAAGCCGAGGCCACCGAAAACGTCG
TCGAGCTCGTCGAAGGTGGTGCCCAAGCGCCGACGGAAAGCGTGGTGGCCGCGGGCCTGGAGGCGGCCAAGCCGTTTATC
GCCGCGCTGTGCACCGCGCAGCAGGAGCTTGCCGATGCCGCTGGAAAGTCGGGCAAACCGACCGTCGACTTCCCGGTGTT
CCCTGACTACGGCGAAGACGTGTACTACTCGGTGTCCTCGGTGGCCACCGACGAGTTGGCCGCCGCGTTGACCATCGGCG
GTAAAGCCGAGCGCGACCAGCGCATCGACGAAATCAAGACCCAGGTTGTGCAGCGGCTCGCCGACACCTACGAGGGTCGC
GAAAAGGAGGTCGGCGCCGCGTTGCGTGCCCTGACCAAAAAGCTGGTTCGGCAGCGCATCCTCACCGACCATTTCCGTAT
CGACGGCCGCGGCATCACCGACATTCGCGCATTGTCGGCCGAGGTGGCCGTGGTTCCGCGCGCGCACGGCAGCGCGCTGT
TCGAACGCGGCGAAACCCAGATCCTGGGTGTGACCACACTCGACATGATCAAGATGGCCCAGCAGATCGACTCGTTGGGG
CCGGAGACATCGAAGCGGTACATGCACCACTACAACTTCCCGCCGTTCTCCACCGGCGAGACCGGTCGGGTCGGTTCGCC
CAAGCGGCGTGAGATCGGGCACGGCGCACTGGCCGAGCGGGCCCTGGTGCCGGTGTTGCCGAGCGTCGAGGAATTCCCGT
ATGCCATTCGCCAGGTGTCGGAGGCTCTGGGCTCCAACGGGTCGACCTCGATGGGGTCGGTGTGCGCGTCGACGCTGGCG
CTGCTCAACGCCGGGGTGCCGCTCAAGGCGCCGGTGGCCGGCATCGCGATGGGCCTGGTCTCCGACGACATTCAAGTAGA
AGGGGCGGTCGACGGCGTTGTGGAGCGTCGCTTCGTCACCCTCACCGACATCCTCGGCGCCGAAGACGCGTTCGGTGACA
TGGACTTCAAGGTCGCCGGGACCAAGGACTTCGTCACCGCGCTGCAGCTGGACACCAAGCTCGACGGGATCCCTTCGCAG
GTGCTTGCCGGAGCACTCGAGCAGGCCAAGGACGCCCGCCTCACGATCTTGGAGGTGATGGCTGAGGCCATCGATAGACC
CGACGAAATGAGTCCCTACGCCCCGCGGGTGACCACCATCAAGGTTCCGGTGGACAAGATCGGGGAGGTCATCGGACCCA
AGGGCAAGGTCATCAACGCCATCACCGAGGAGACCGGCGCGCAGATCTCCATCGAAGACGACGGCACCGTGTTCGTCGGC
GCCACCGACGGGCCATCGGCACAGGCCGCGATCGACAAGATCAACGCCATCGCCAACCCGCAGCTGCCGACGGTGGGCGA
ACGGTTCCTCGGAACCGTGGTCAAGACCACCGATTTCGGTGCCTTTGTATCGTTGCTGCCTGGCCGCGACGGTCTGGTGC
ACATTTCCAAACTCGGCAAGGGCAAGCGCATCGCGAAGGTCGAGGACGTTGTCAATGTCGGTGACAAGCTGCGGGTGGAG
ATCGCCGACATCGACAAACGGGGCAAGATCTCCCTGATCCTGGTCGCCGACGAGGACAGCACCGCCGCCGCTACCGATGC
CGCGACGGTCACCAGCTGA

Upstream 100 bases:

>100_bases
GACCTTTTCTCCTGGGAAGCTCTCGGGGTTCGGCGAACCCGCTGGATTGCGCGTGACGACGCGAAACAGCTCAATAATCC
AGAGAGGCCGCACGGACGTC

Downstream 100 bases:

>100_bases
CCCCGCGGCGGCGCTGGCGCCGCGGCGCACCACCCTGCCGGGCGGGCTGCGAGTGGTCACCGAATTCCTGCCCGCGGTGC
ACTCCGCGTCGGTCGGGGTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSATTASKNPKEHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDVAIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFI
AALCTAQQELADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQRIDEIKTQVVQRLADTYEGR
EKEVGAALRALTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLG
PETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCASTLA
LLNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQ
VLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVG
ATDGPSAQAAIDKINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVE
IADIDKRGKISLILVADEDSTAAATDAATVTS

Sequences:

>Translated_752_residues
MSAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSATTASKNPKEHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDVAIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFI
AALCTAQQELADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQRIDEIKTQVVQRLADTYEGR
EKEVGAALRALTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLG
PETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCASTLA
LLNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQ
VLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVG
ATDGPSAQAAIDKINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVE
IADIDKRGKISLILVADEDSTAAATDAATVTS
>Mature_751_residues
SAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSATTASKNPKEHFDFFPLTVDVEERMYA
AGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASASTQLGGLPFSGPI
GGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDVAIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFIA
ALCTAQQELADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQRIDEIKTQVVQRLADTYEGRE
KEVGAALRALTKKLVRQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGP
ETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCASTLAL
LNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQV
LAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVGA
TDGPSAQAAIDKINAIANPQLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVEI
ADIDKRGKISLILVADEDSTAAATDAATVTS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=695, Percent_Identity=35.5395683453237, Blast_Score=379, Evalue=1e-105,
Organism=Escherichia coli, GI145693187, Length=714, Percent_Identity=45.2380952380952, Blast_Score=569, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI115534063, Length=728, Percent_Identity=32.5549450549451, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=35.9440559440559, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=35.9440559440559, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=35.9440559440559, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI161079377, Length=659, Percent_Identity=35.9635811836115, Blast_Score=349, Evalue=4e-96,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79735; Mature: 79604

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSATTASK
CCCHHHCCCHHHEEEEECCCCCCCEEEEEECCCEEEEECCEEEEEECCCCEEEEEECCCC
NPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEEECCCCCCCHHHHHHH
NEIQIVVTILSLDPGDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVGFPTVD
HCEEEEEEEEECCCCCCEEHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
QIERAVFDMVVAGRIVEGDVAIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFI
HHHHHHHHHHHHCEEEECCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AALCTAQQELADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQ
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHHHHHEEECCCCHHHH
RIDEIKTQVVQRLADTYEGREKEVGAALRALTKKLVRQRILTDHFRIDGRGITDIRALSA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCC
EVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGPETSKRYMHHYNFPPFSTGE
EEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
TGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCASTLA
CCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAG
HHHCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
TKDFVTALQLDTKLDGIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
KVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVGATDGPSAQAAIDKINAIANP
ECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
QLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVE
CCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEE
IADIDKRGKISLILVADEDSTAAATDAATVTS
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSATTASK
CCHHHCCCHHHEEEEECCCCCCCEEEEEECCCEEEEECCEEEEEECCCCEEEEEECCCC
NPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEEECCCCCCCHHHHHHH
NEIQIVVTILSLDPGDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVGFPTVD
HCEEEEEEEEECCCCCCEEHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
QIERAVFDMVVAGRIVEGDVAIMMVEAEATENVVELVEGGAQAPTESVVAAGLEAAKPFI
HHHHHHHHHHHHCEEEECCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AALCTAQQELADAAGKSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQ
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHHHHHEEECCCCHHHH
RIDEIKTQVVQRLADTYEGREKEVGAALRALTKKLVRQRILTDHFRIDGRGITDIRALSA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCC
EVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGPETSKRYMHHYNFPPFSTGE
EEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
TGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCASTLA
CCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAG
HHHCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
TKDFVTALQLDTKLDGIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
KVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVGATDGPSAQAAIDKINAIANP
ECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCC
QLPTVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLRVE
CCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEE
IADIDKRGKISLILVADEDSTAAATDAATVTS
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA