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The map label for this gene is miaB [H]

Identifier: 148823920

GI number: 148823920

Start: 3056330

End: 3056944

Strand: Reverse

Name: miaB [H]

Synonym: TBFG_12745

Alternate gene names: 148823920

Gene position: 3056944-3056330 (Counterclockwise)

Preceding gene: 148823921

Following gene: 148823917

Centisome position: 69.09

GC content: 68.46

Gene sequence:

>615_bases
ATGATGAGCCACGAACACGACGCCGGGGATCTCGACGCCCTCCGCGCTGAGATCGAGGCGGCAGAACGCCGCGTTGCGCG
CGAAATCGAGCCAGGCGCGAGGGCTTTGGTTGTCGCAATCCTGGTGTTCGTGCTGTTGGGGTCGTTTATCCTGCCGCATA
CCGGAAGCGTGCGCGGCTGGGATGTGTTGTTCAGCAGCCACGGCGCCGGCAGGGCCGCCGTCGCGCTGCCCTCGCGGGTA
TTCGCCTGGTTGGCGCTGGTGTTCGGCGTTGGCTTCTCGATGCTGGCGTTGCTCACCCGGCGGTGGGCGCTGGCTTGGGT
GGCGCTGGCGGGTTCGGCGATGGCCAGCGGTACCGGGCTGCTGGCCGTATGGTCGCGTCAGACGGTCGCCGCCGGTCATC
CCGGCCCCGGTATCGGGCTCATCGTCGCCTGGATCACCGCGATCGTGTTGACATTCCACTGGGCCCAGGTGGTGTGGTCG
CGCACCATCGTGCAGCTCGCAGCCGAGGAGCGGCGTCGCCGCGTCGTCGCGCAACAACAGTGCAAGACGCTGCTGGACCA
CGTGCAAACGGACTCCGAGGCCGGGACAACGCCCGACCGCGGCACCGACCGTTAG

Upstream 100 bases:

>100_bases
CCGGCGACGCGCACACCGCCGGGCAGCCGGGGCGCGCCGTCGGCCTGGGCATGCCTGGGGTCGGGCTGCCGGTCAGCGCG
GCCAAGCCCGGAGGATGCAG

Downstream 100 bases:

>100_bases
GGGCGTCGGGTCAACGCGTCGGCGGCTGCCTCGGCCCACTGCCGCCATTGTTCGGCGTTCGCCTTCGCCTCGTCGGCTTC
CTTGGTGCGACCGGCCGCTG

Product: transmembrane protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MMSHEHDAGDLDALRAEIEAAERRVAREIEPGARALVVAILVFVLLGSFILPHTGSVRGWDVLFSSHGAGRAAVALPSRV
FAWLALVFGVGFSMLALLTRRWALAWVALAGSAMASGTGLLAVWSRQTVAAGHPGPGIGLIVAWITAIVLTFHWAQVVWS
RTIVQLAAEERRRRVVAQQQCKTLLDHVQTDSEAGTTPDRGTDR

Sequences:

>Translated_204_residues
MMSHEHDAGDLDALRAEIEAAERRVAREIEPGARALVVAILVFVLLGSFILPHTGSVRGWDVLFSSHGAGRAAVALPSRV
FAWLALVFGVGFSMLALLTRRWALAWVALAGSAMASGTGLLAVWSRQTVAAGHPGPGIGLIVAWITAIVLTFHWAQVVWS
RTIVQLAAEERRRRVVAQQQCKTLLDHVQTDSEAGTTPDRGTDR
>Mature_204_residues
MMSHEHDAGDLDALRAEIEAAERRVAREIEPGARALVVAILVFVLLGSFILPHTGSVRGWDVLFSSHGAGRAAVALPSRV
FAWLALVFGVGFSMLALLTRRWALAWVALAGSAMASGTGLLAVWSRQTVAAGHPGPGIGLIVAWITAIVLTFHWAQVVWS
RTIVQLAAEERRRRVVAQQQCKTLLDHVQTDSEAGTTPDRGTDR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 64 in tRNAs that read codons beginning with uridine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 21990; Mature: 21990

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSHEHDAGDLDALRAEIEAAERRVAREIEPGARALVVAILVFVLLGSFILPHTGSVRGW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DVLFSSHGAGRAAVALPSRVFAWLALVFGVGFSMLALLTRRWALAWVALAGSAMASGTGL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LAVWSRQTVAAGHPGPGIGLIVAWITAIVLTFHWAQVVWSRTIVQLAAEERRRRVVAQQQ
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CKTLLDHVQTDSEAGTTPDRGTDR
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMSHEHDAGDLDALRAEIEAAERRVAREIEPGARALVVAILVFVLLGSFILPHTGSVRGW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DVLFSSHGAGRAAVALPSRVFAWLALVFGVGFSMLALLTRRWALAWVALAGSAMASGTGL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LAVWSRQTVAAGHPGPGIGLIVAWITAIVLTFHWAQVVWSRTIVQLAAEERRRRVVAQQQ
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CKTLLDHVQTDSEAGTTPDRGTDR
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA