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The map label for this gene is plcA

Identifier: 148823552

GI number: 148823552

Start: 2641334

End: 2642896

Strand: Reverse

Name: plcA

Synonym: TBFG_12375

Alternate gene names: 148823552

Gene position: 2642896-2641334 (Counterclockwise)

Preceding gene: 148823553

Following gene: 148823551

Centisome position: 59.73

GC content: 62.96

Gene sequence:

>1563_bases
GTGAGCGCAAGCCCACTCCTCGGAATGTCACGTCGAGAGTTTTTGACAAAGCTCACTGGCGCAGGCGCAGCGGCATTCCT
GATGGACTGGGCTGCACCGGTGATTGAAAAGGCCTACGGCGCCGGGCCTTGTCCCGGACATTTGACCGACATCGAGCATA
TCGTGTTGCTGATGCAGGAGAACCGGTCATTCGACCACTATTTCGGAACGCTTTCCAGCACCAATGGGTTCAACGCCGCG
TCGCCGGCATTCCAACAAATGGGTTGGAACCCCATGACGCAGGCGTTGGACCCCGCCGGGGTCACCATTCCGTTCCGCTT
GGACACCACCCGAGGCCCCTTCCTGGACGGCGAGTGCGTCAACGACCCCGAGCACCAGTGGGTGGGGATGCACCTGGCCT
GGAACGGTGGTGCCAACGACAACTGGCTGCCGGCGCAGGCGACCACCCGCGCAGGACCATATGTCCCTTTGACCATGGGT
TACTACACGCGCCAAGACATCCCGATCCACTATCTGCTGGCCGACACGTTCACCATCTGCGACGGCTACCATTGCTCGCT
GCTGACGGGCACCCTGCCCAACCGGCTCTACTGGTTGAGCGCCAACATCGACCCCGCCGGCACCGACGGGGGACCCCAAT
TGGTAGAGCCGGGCTTCCTGCCGCTGCAGCAATTCAGTTGGCGCATCATGCCGGAAAACCTCGAAGATGCCGGGGTCAGC
TGGAAGGTGTACCAGAACAAGGGCCTCGGGCGATTCATCAACACGCCCATCAGCAATAACGGGCTGGTGCAGGCCTTCCG
CCAGGCAGCTGATCCGAGGTCGAACTTGGCCCGCTACGGTATCGCCCCGACCTACCCTGGGGACTTCGCTGCCGACGTCA
GGGCCAACCGGCTACCCAAGGTCTCCTGGTTAGTTCCCAACATCCTGCAGTCCGAACACCCCGCCCTGCCGGTAGCGCTT
GGCGCGGTGTCCATGGTGACCGCGCTGCGGATCTTGCTGTCCAATCCCGCGGTGTGGGAAAAGACCGCACTTATCGTCAG
CTATGACGAGAACGGCGGCTTCTTCGACCACGTCACGCCCCCCACGGCACCGCCCGGGACACCCGGCGAATTCGTCACGG
TGCCCAACATCGACGCAGTACCCGGGTCCGGTGGCATTCGTGGTCCGCTCGGTCTGGGTTTTCGCGTTCCCTGCATTGTC
ATTTCGCCGTACAGCCGCGGCCCGCTGATGGTCTCCGACACGTTCGACCACACCTCGCAATTGAAGTTGATTCGCGCCCG
GTTCGGCGTGCCGGTTCCCAACATGACCGCCTGGCGCGACGGCGTGGTTGGCGACATGACCTCAGCGTTCAACTTTGCGA
CTCCACCGAATTCGACCAGACCCAACTTGAGCCACCCGTTGCTGGGAGCGCTGCCGAAGCTGCCGCAGTGCATCCCTAAC
GTGGTGTTGGGAACCACCGACGGCGCGTTGCCGAGCATTCCCTATCGGGTGCCCTATCCGCAGGTGATGCCAACTCAGGA
AACCACACCCGTCCGCGGGACTCCCAGCGGGCTGTGCAGCTGA

Upstream 100 bases:

>100_bases
GAACGCGAAAATCCGCGTGCGACACGCCGGTTGGCGTATCGCGATGGACCGCTGTCGTCACTATAACGTGATCTGAGCTG
AGTGCAGGAAGGCAGGGCAA

Downstream 100 bases:

>100_bases
AATTTCCCGGTAATCTCCCGGCGTTCGATTTGCTTGCATGTGCGCGAGCAAATCGCCCGCACCAATCCCGCGCCCGCCCC
GGCAGGCAGGCGGAATCAGA

Product: membrane-associated phospholipase C 1 plcA

Products: NA

Alternate protein names: MTP40 antigen

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSASPLLGMSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAA
SPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMG
YYTRQDIPIHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVS
WKVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVAL
GAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIV
ISPYSRGPLMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPN
VVLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS

Sequences:

>Translated_520_residues
MSASPLLGMSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAA
SPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMG
YYTRQDIPIHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVS
WKVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVAL
GAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIV
ISPYSRGPLMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPN
VVLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
>Mature_519_residues
SASPLLGMSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAAS
PAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGY
YTRQDIPIHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSW
KVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVALG
AVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVI
SPYSRGPLMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNV
VLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHLA_MYCTU (Q04001)

Other databases:

- EMBL:   L11868
- EMBL:   L11868
- EMBL:   U49511
- EMBL:   BX842579
- EMBL:   AE000516
- EMBL:   M57952
- EMBL:   S69737
- PIR:   H70662
- RefSeq:   NP_216867.1
- RefSeq:   NP_336895.1
- EnsemblBacteria:   EBMYCT00000002239
- EnsemblBacteria:   EBMYCT00000070413
- GeneID:   885995
- GeneID:   925941
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2416
- KEGG:   mtu:Rv2351c
- TIGR:   MT2416
- TubercuList:   Rv2351c
- GeneTree:   EBGT00050000017446
- HOGENOM:   HBG565180
- OMA:   FINTPIS
- ProtClustDB:   CLSK791798
- BRENDA:   3.1.4.3
- GO:   GO:0009405
- InterPro:   IPR007312
- InterPro:   IPR006311
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04185 Phosphoesterase

EC number: =3.1.4.3

Molecular weight: Translated: 56139; Mature: 56007

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPLLGMSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQE
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEEC
NRSFDHYFGTLSSTNGFNAASPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECV
CCCCHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
NDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPIHYLLADTFTIC
CCCCCEEEEEEEEECCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCEEEEEECHHHHC
DGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVS
CCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCE
WKVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPK
EEEECCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCC
VSWLVPNILQSEHPALPVALGAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTP
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCC
PTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPLMVSDTFDHTSQ
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEECCCCCHHH
LKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPN
HHHHHHHHCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
VVLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SASPLLGMSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQE
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEEC
NRSFDHYFGTLSSTNGFNAASPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECV
CCCCHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
NDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPIHYLLADTFTIC
CCCCCEEEEEEEEECCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCEEEEEECHHHHC
DGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVS
CCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCE
WKVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPK
EEEECCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCC
VSWLVPNILQSEHPALPVALGAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTP
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCC
PTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPLMVSDTFDHTSQ
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEECCCCCHHH
LKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPN
HHHHHHHHCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
VVLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7591062; 8757862; 9634230; 12218036; 1909999; 8298518; 9114405