Click here to switch to the map view.

The map label for this gene is plcB

Identifier: 148823551

GI number: 148823551

Start: 2639578

End: 2641143

Strand: Reverse

Name: plcB

Synonym: TBFG_12374

Alternate gene names: 148823551

Gene position: 2641143-2639578 (Counterclockwise)

Preceding gene: 148823552

Following gene: 148823550

Centisome position: 59.69

GC content: 62.84

Gene sequence:

>1566_bases
GTGGGGAGCGAACACCCCGTCGACGGAATGACCCGCCGACAATTTTTTGCCAAAGCCGCCGCCGCTACCACCGCCGGGGC
CTTCATGTCCTTGGCTGGTCCGATTATCGAAAAAGCCTACGGAGCGGGGCCTTGCCCCGGACATTTGACCGACATCGAGC
ACATCGTGTTGTTGATGCAGGAGAATCGGTCATTCGATCACTACTTCGGCACTCTTTCTGACACCCGCGGGTTCGATGAC
ACCACCCCGCCGGTGGTATTCGCGCAGTCGGGCTGGAACCCGATGACACAGGCGGTTGACCCCGCCGGCGTCACCCTTCC
ATATCGCTTCGACACCACCCGGGGCCCGCTGGTCGCCGGCGAATGCGTCAACGACCCGGACCACAGCTGGATCGGCATGC
ACAACTCGTGGAACGGCGGCGCCAACGACAACTGGCTGCCGGCGCAGGTCCCGTTCAGTCCGTTGCAGGGCAACGTGCCG
GTCACGATGGGTTTCTACACGCGTCGTGACCTGCCCATTCACTACCTGCTAGCCGACACGTTCACGGTCTGCGACGGCTA
TTTTTGCTCGCTGCTGGGCGGGACCACGCCCAACCGGCTCTACTGGATGAGCGCCTGGATCGACCCCGACGGCACTGACG
GCGGGCCGGTGCTGATCGAGCCCAATATCCAACCTCTGCAGCACTACAGCTGGCGCATCATGCCGGAGAACCTCGAAGAT
GCCGGGGTCAGCTGGAAGGTGTACCAAAACAAATTGCTGGGGGCTCTCAACAACACCGTCGTCGGCTACAACGGGCTGGT
CAATGACTTCAAGCAGGCCGCGGATCCGAGGTCGAATCTGGCCCGTTTCGGCATCTCACCGACCTACCCGCTGGACTTCG
CCGCCGACGTCAGAAACAACCGATTACCCAAGGTCTCCTGGGTGCTGCCCGGGTTTCTGCTGTCCGAACATCCCGCATTC
CCGGTGAACGTCGGCGCCGTTGCAATTGTCGATGCACTGCGGATCTTGCTGTCCAACCCTGCGGTGTGGGAAAAGACCGC
GCTGATCGTCAACTACGACGAGAACGGCGGGTTCTTCGACCACGTCGTGCCGCCGACACCGCCGCCGGGGACGCCCGGCG
AATTCGTCACGGTCCCCGACATCGACTCGGTGCCCGGCTCGGGTGGCATTCGTGGGCCGATCGGTCTGGGGTTTCGCGTC
CCTTGCCTCGTCATCTCTCCCTATAGCCGCGGCCCACTGATGGTCCACGACACCTTCGATCACACGTCAACGCTGAAGCT
CATCCGCGCGCGGTTCGGCGTCCCGGTTCCCAATCTCACCGCTTGGCGGGACGCGACGGTCGGCGATATGACTTCGACGT
TCAACTTCGCCGCTCCGCCGAACCCGTCGAAACCCAATCTGGACCACCCGCGGCTCAATGCGCTTCCGAAGTTGCCGCAG
TGCGTGCCCAACGCAGTACTGGGAACGGTGACCAAGACGGCAATTCCCTATCGGGTGCCATTCCCACAGTCGATGCCCAC
CCAAGAAACCGCACCCACTCGGGGGATTCCCAGCGGTCTCTGTTGA

Upstream 100 bases:

>100_bases
CGGAATCAGAACACGACACGCCGGTCGGCAAGGCATAGCCGATCGCGGCCGCAACTATACCGTGATCAGCAATGGCCGTG
TGGGCCGTCAAGGAGGGCGC

Downstream 100 bases:

>100_bases
CCCACAGTCGGCGATGGCCGCGACGGTAAGCCGAAATACACGAGGGAGAGCCGATGTGGTGAGCCAAGGTGCATTCGCCG
GAATGTCACGCCGAGCATTC

Product: membrane-associated phospholipase C 2 plcB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MGSEHPVDGMTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDD
TTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVP
VTMGFYTRRDLPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLED
AGVSWKVYQNKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAF
PVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRV
PCLVISPYSRGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQ
CVPNAVLGTVTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC

Sequences:

>Translated_521_residues
MGSEHPVDGMTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDD
TTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVP
VTMGFYTRRDLPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLED
AGVSWKVYQNKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAF
PVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRV
PCLVISPYSRGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQ
CVPNAVLGTVTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
>Mature_520_residues
GSEHPVDGMTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDT
TPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPV
TMGFYTRRDLPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDA
GVSWKVYQNKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAFP
VNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVP
CLVISPYSRGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQC
VPNAVLGTVTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHLB_MYCTU (P95246)

Other databases:

- EMBL:   U49511
- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   G70662
- RefSeq:   NP_216866.1
- RefSeq:   NP_336894.1
- ProteinModelPortal:   P95246
- EnsemblBacteria:   EBMYCT00000000881
- EnsemblBacteria:   EBMYCT00000069287
- GeneID:   885999
- GeneID:   925938
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2415
- KEGG:   mtu:Rv2350c
- TIGR:   MT2415
- TubercuList:   Rv2350c
- GeneTree:   EBGT00050000017446
- HOGENOM:   HBG565180
- OMA:   KAGISWQ
- ProtClustDB:   CLSK791798
- BRENDA:   3.1.4.3
- GO:   GO:0009405
- InterPro:   IPR007312
- InterPro:   IPR006311
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04185 Phosphoesterase

EC number: =3.1.4.3

Molecular weight: Translated: 56534; Mature: 56403

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEHPVDGMTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEE
ENRSFDHYFGTLSDTRGFDDTTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAG
CCCCHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCEEEC
ECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRRDLPIHYLLADT
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHH
FTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLED
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHCCCEEEECCCCCCC
AGVSWKVYQNKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNN
CCCEEEEHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCC
RLPKVSWVLPGFLLSEHPAFPVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFD
CCCEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEC
HVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYSRGPLMVHDTFD
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEEECCC
HTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQ
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CVPNAVLGTVTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GSEHPVDGMTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEE
ENRSFDHYFGTLSDTRGFDDTTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAG
CCCCHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCEEEC
ECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRRDLPIHYLLADT
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHH
FTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLED
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHCCCEEEECCCCCCC
AGVSWKVYQNKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNN
CCCEEEEHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCC
RLPKVSWVLPGFLLSEHPAFPVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFD
CCCEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEC
HVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYSRGPLMVHDTFD
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEEECCC
HTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQ
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CVPNAVLGTVTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8757862; 9634230; 12218036