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The map label for this gene is 148822645

Identifier: 148822645

GI number: 148822645

Start: 1603999

End: 1605378

Strand: Direct

Name: 148822645

Synonym: TBFG_11454

Alternate gene names: NA

Gene position: 1603999-1605378 (Clockwise)

Preceding gene: 148822643

Following gene: 148822649

Centisome position: 36.25

GC content: 65.36

Gene sequence:

>1380_bases
ATGAAGCGGCTTTCGAGCGTTGATGCTGCGTTTTGGTCCGCGGAAACCGCAGGCTGGCATATGCACGTGGGCGCACTGGC
GATCTGCGATCCCAGCGACGCGCCCGAATACAGCTTTCAGCGGCTCCGCGAGTTGATCATCGAACGGCTGCCGGAGATCC
CGCAGTTGCGGTGGCGGGTCACCGGCGCCCCGCTCGGACTGGACCGGCCGTGGTTCGTCGAGGACGAGGAACTCGACATC
GACTTTCACATCCGCCGCATCGGTGTTCCGGCTCCCGGTGGGCGGCGCGAACTCGAGGAGCTCGTCGGACGGCTGATGTC
CTACAAACTGGACCGTTCCCGGCCGCTGTGGGAACTGTGGGTCATCGAGGGCGTCGAGGGCGGCCGCATCGCCACGCTGA
CCAAGATGCATCACGCCATCGTCGACGGTGTCTCCGGTGCCGGGCTGGGCGAAATCCTGTTGGACATCACACCAGAACCA
CGACCACCGCAACAGGAAACGGTCGGCTTCGTGGGATTCCAGATTCCGGGCCTGGAACGCCGGGCGATAGGTGCGCTGAT
CAACGTGGGCATCATGACGCCCTTCCGCATCGTCAGGCTGCTGGAGCAAACCGTGCGTCAACAGATCGCGGCATTGGGTG
TGGCCGGCAAACCGGCGCGATACTTCGAAGCGCCCAAGACGCGGTTCAATGCGCCGGTGTCGCCGCACCGGCGGGTTACC
GGCACACGCGTCGAGCTGGCTAGGGCCAAAGCGGTCAAGGACGCGTTCGGCGTCAAGCTCAACGACGTCGTCTTGGCGCT
GGTGGCCGGGGCGGCCCGGCAATACCTACAGAAGCGTGACGAGCTGCCCGCCAAGCCGTTGATCGCGCAGATTCCGGTCT
CCACCCGCAGCGAGGAAACGAAGGCCGACGTCGGGAACCAGGTCAGCTCGATGACCGCGTCGCTGGCAACCCATATCGAG
GATCCGGCCAAGCGCCTGGCGGCCATCCACGAGAGCACCCTCAGCGCCAAGGAAATGGCTAAGGCGCCCTCCGCGCACCA
GATCATGGGGCTGACCGAGACCACGCCACCGGGTCTGCTGCAGCTGGCCGCCCGGGCCTATACGGCCAGCGGGCTGTCAC
ACAACCTGGCCCCAATCAACCTCGTCGTCTCCAATGTCCCCGGTCCACCCTTCCCGCTATATATGGCCGGCGCGCGGCTG
GATTCGCTGGTGCCCCTGGGGCCGCCGGTGATGGACGTGGCGCTGAACATCACCTGCTTCTCCTACCAGGATTATCTGGA
TTTCGGCCTGGTGACCACACCCGAGGTGGCCAACGACATCGACGAGATGGCCGATGCCATCGAACCGGCACTGGCCGAGC
TGGAGCGTGCCGCGGAATAG

Upstream 100 bases:

>100_bases
TCGCCGATGTTTGGGCCGAAGCTTGCACACATTGACGATAACTTGGTCGCGAGACCGCAGAAGCTGGCCTCGACGGCGCG
CCGGGGACTACGGTCATACC

Downstream 100 bases:

>100_bases
CAATAGCTGGCCTATAGCTGACTACGTGGCCGGCGGGTTGGTCGCGTACACCCAAGACAGGAAGCGGGCCACGGCCTCGG
CGGTGTGATGCGCCCGCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKAPSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE

Sequences:

>Translated_459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKAPSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
>Mature_459_residues
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRVTGAPLGLDRPWFVEDEELDI
DFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELWVIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEP
RPPQQETVGFVGFQIPGLERRAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEETKADVGNQVSSMTASLATHIE
DPAKRLAAIHESTLSAKEMAKAPSAHQIMGLTETTPPGLLQLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARL
DSLVPLGPPVMDVALNITCFSYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1425_MYCTU (P71694)

Other databases:

- EMBL:   BX842576
- EMBL:   AE000516
- PIR:   D70914
- RefSeq:   NP_215941.1
- RefSeq:   NP_335919.1
- ProteinModelPortal:   P71694
- EnsemblBacteria:   EBMYCT00000002510
- EnsemblBacteria:   EBMYCT00000072948
- GeneID:   886668
- GeneID:   924503
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1468
- KEGG:   mtu:Rv1425
- TIGR:   MT1468
- TubercuList:   Rv1425
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   RNLAPIN
- ProtClustDB:   CLSK791155
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 50063; Mature: 50063

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRV
CCCCHHHHHHHHCCCCCCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
TGAPLGLDRPWFVEDEELDIDFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELW
ECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
VIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEPRPPQQETVGFVGFQIPGLER
EEECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCHHHHCEEEEEECCCCHH
RAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCCCCCCCCCC
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEET
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
KADVGNQVSSMTASLATHIEDPAKRLAAIHESTLSAKEMAKAPSAHQIMGLTETTPPGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHH
QLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARLDSLVPLGPPVMDVALNITCF
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCHHHHEEEEEEE
SYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLSSVDAAFWSAETAGWHMHVGALAICDPSDAPEYSFQRLRELIIERLPEIPQLRWRV
CCCCHHHHHHHHCCCCCCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
TGAPLGLDRPWFVEDEELDIDFHIRRIGVPAPGGRRELEELVGRLMSYKLDRSRPLWELW
ECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
VIEGVEGGRIATLTKMHHAIVDGVSGAGLGEILLDITPEPRPPQQETVGFVGFQIPGLER
EEECCCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCHHHHCEEEEEECCCCHH
RAIGALINVGIMTPFRIVRLLEQTVRQQIAALGVAGKPARYFEAPKTRFNAPVSPHRRVT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCCCCCCCCCC
GTRVELARAKAVKDAFGVKLNDVVLALVAGAARQYLQKRDELPAKPLIAQIPVSTRSEET
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHH
KADVGNQVSSMTASLATHIEDPAKRLAAIHESTLSAKEMAKAPSAHQIMGLTETTPPGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCHHHH
QLAARAYTASGLSHNLAPINLVVSNVPGPPFPLYMAGARLDSLVPLGPPVMDVALNITCF
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCHHHHEEEEEEE
SYQDYLDFGLVTTPEVANDIDEMADAIEPALAELERAAE
EHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036