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The map label for this gene is folE

Identifier: 148663473

GI number: 148663473

Start: 4058642

End: 4059250

Strand: Reverse

Name: folE

Synonym: MRA_3648

Alternate gene names: 148663473

Gene position: 4059250-4058642 (Counterclockwise)

Preceding gene: 148663474

Following gene: 148663472

Centisome position: 91.84

GC content: 61.9

Gene sequence:

>609_bases
ATGTCGCAGCTGGATTCGCGCAGCGCATCTGCTCGTATCCGTGTGTTCGACCAGCAACGTGCCGAGGCCGCGGTGCGCGA
ATTGCTGTACGCGATCGGCGAGGATCCGGATAGGGACGGCTTGGTAGCCACCCCGTCCCGGGTTGCCCGGTCATACCGCG
AAATGTTCGCCGGGCTCTACACCGACCCCGACTCGGTGTTGAACACCATGTTCGACGAAGACCACGACGAGCTGGTGTTG
GTCAAGGAAATCCCTATGTACTCCACCTGCGAACACCACCTGGTGGCGTTCCACGGTGTGGCCCACGTCGGCTACATCCC
GGGCGACGACGGCAGGGTGACCGGCTTGTCAAAGATCGCGCGACTGGTCGATCTGTACGCCAAGCGACCTCAGGTCCAGG
AGCGGCTCACCAGTCAGATCGCCGATGCCCTGATGAAAAAACTCGATCCACGCGGGGTAATCGTGGTGATCGAGGCTGAG
CATCTGTGCATGGCGATGCGCGGGGTTCGCAAGCCCGGCTCGGTCACCACTACGTCGGCGGTGCGCGGACTGTTCAAAAC
CAATGCCGCTTCTCGAGCCGAAGCGCTCGACCTCATTTTGCGGAAGTGA

Upstream 100 bases:

>100_bases
GACCGGTCAAGCCGATCCGGGTTCCGATGAGTCCTCGGCGGAGCAGGATGACGAGGTCAGTCGGACCAAGCCGGCCCACG
GCTGACGAACGGAGCATTCG

Downstream 100 bases:

>100_bases
GTCCGGCGCCCGTGCAGGTGATGGGGGTTCTAAACGTCACGGACGACTCTTTCTCGGACGGCGGGTGTTATCTCGATCTC
GACGATGCGGTGAAGCACGG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLYTDPDSVLNTMFDEDHDELVL
VKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAE
HLCMAMRGVRKPGSVTTTSAVRGLFKTNAASRAEALDLILRK

Sequences:

>Translated_202_residues
MSQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLYTDPDSVLNTMFDEDHDELVL
VKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAE
HLCMAMRGVRKPGSVTTTSAVRGLFKTNAASRAEALDLILRK
>Mature_201_residues
SQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLYTDPDSVLNTMFDEDHDELVLV
KEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAEH
LCMAMRGVRKPGSVTTTSAVRGLFKTNAASRAEALDLILRK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=178, Percent_Identity=50.561797752809, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI4503949, Length=178, Percent_Identity=50.561797752809, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI66932970, Length=138, Percent_Identity=51.4492753623188, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=51.4492753623188, Blast_Score=137, Evalue=5e-33,
Organism=Escherichia coli, GI1788476, Length=172, Percent_Identity=44.1860465116279, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=48.8888888888889, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=48.9247311827957, Blast_Score=176, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=47.7777777777778, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=47.7777777777778, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=47.7777777777778, Blast_Score=162, Evalue=1e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_MYCBO (P64208)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857278.1
- ProteinModelPortal:   P64208
- SMR:   P64208
- EnsemblBacteria:   EBMYCT00000016062
- GeneID:   1093567
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3639c
- GeneTree:   EBGT00050000016683
- HOGENOM:   HBG370191
- OMA:   MAPIIGR
- ProtClustDB:   PRK09347
- BioCyc:   MBOV233413:MB3639C-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22395; Mature: 22264

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLY
CCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHCC
TDPDSVLNTMFDEDHDELVLVKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAEHLCMAMRGVRKPGSVTTTSA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCCHHHHH
VRGLFKTNAASRAEALDLILRK
HHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLY
CCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHCC
TDPDSVLNTMFDEDHDELVLVKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIA
CCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAEHLCMAMRGVRKPGSVTTTSA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCCHHHHH
VRGLFKTNAASRAEALDLILRK
HHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972