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The map label for this gene is lysS

Identifier: 148663463

GI number: 148663463

Start: 4050088

End: 4051605

Strand: Reverse

Name: lysS

Synonym: MRA_3638

Alternate gene names: 148663463

Gene position: 4051605-4050088 (Counterclockwise)

Preceding gene: 148663464

Following gene: 148663462

Centisome position: 91.67

GC content: 60.54

Gene sequence:

>1518_bases
GTGAGTGCCGCTGACACAGCAGAAGACCTTCCTGAGCAGTTCCGGATTCGCCGGGACAAGCGCGCTCGCTTGCTGGCCCA
GGGGCGCGATCCCTATCCCGTCGCGGTGCCGCGCACTCACACGTTGGCCGAGGTTCGCGCCGCCCACCCTGACTTGCCGA
TCGATACCGCGACCGAAGACATCGTCGGCGTCGCGGGCCGAGTGATCTTTGCGCGCAACTCGGGAAAGCTATGCTTTGCG
ACACTTCAGGACGGCGATGGTACCCAGCTGCAAGTGATGATCAGCCTCGACAAGGTCGGCCAGGCTGCTCTCGACGCATG
GAAAGCCGATGTCGACCTGGGCGACATCGTCTACGTGCATGGCGCGGTGATCAGTTCGCGCCGCGGCGAGCTGTCCGTCC
TGGCGGATTGCTGGCGGATCGCCGCCAAGTCGCTGCGGCCGCTTCCCGTCGCGCACAAAGAGATGAGTGAAGAGTCGCGG
GTTCGTCAGCGCTATGTTGACCTCATAGTTCGACCGGAAGCGCGCGCGGTGGCTCGACTACGGATCGCCGTCGTCCGCGC
GATCCGGACGGCGCTTCAACGTCGTGGGTTCCTGGAAGTCGAGACGCCCGTCTTGCAGACGTTAGCCGGTGGTGCGGCGG
CCCGTCCGTTCGCCACTCATTCCAATGCCCTAGACATCGATCTGTACCTGCGGATCGCGCCGGAACTGTTCCTCAAGCGC
TGCATCGTGGGTGGTTTCGACAAGGTCTTCGAACTTAATCGAGTGTTCCGAAACGAAGGAGCCGATTCCACGCATTCTCC
GGAATTCTCCATGCTGGAGACCTACCAGACCTACGGAACCTATGACGATTCGGCAGTCGTCACCCGGGAGCTTATTCAAG
AGGTGGCCGATGAGGCGATCGGAACCAGACAACTGCCGTTGCCCGACGGCAGTGTCTATGACATCGACGGAGAATGGGCG
ACTATACAAATGTACCCGTCGCTGTCTGTGGCGCTCGGTGAAGAGATCACACCGCAGACGACGGTCGATCGCTTACGTGG
GATCGCCGATAGCCTTGGCCTGGAGAAAGACCCAGCGATTCATGACAACCGTGGCTTCGGCCACGGCAAACTCATCGAGG
AACTCTGGGAGCGCACAGTGGGCAAGAGCTTGAGCGCACCCACATTTGTCAAGGATTTTCCGGTTCAGACAACGCCTTTG
ACCCGTCAGCACCGCAGTATCCCCGGCGTAACCGAGAAGTGGGACCTCTATCTGCGCGGAATCGAACTTGCCACCGGCTA
CTCGGAATTAAGCGACCCGGTAGTCCAGCGGGAGAGATTCGCCGACCAGGCCCGTGCCGCGGCCGCTGGCGATGACGAAG
CGATGGTGCTTGACGAGGATTTTCTGGCCGCTCTGGAGTACGGCATGCCACCGTGCACCGGAACCGGAATGGGTATCGAT
CGGTTGTTGATGTCTTTGACTGGGTTGTCAATTAGGGAGACAGTTTTGTTCCCGATTGTTCGACCACACTCCAACTGA

Upstream 100 bases:

>100_bases
CGTCGATGCCGGCATCGAGTCTGGGTACCGGGTCGCCCGCCGCCGACAGGCTCGACGCCACCCACGAGCGTCGGCGTGAG
GTCATTTAAGCTGGCACGTC

Downstream 100 bases:

>100_bases
ACTGTGGGTGTTGTGTCTGGATTGAGTATTCTGCGTTCTTATGGCAGATTGGTGACCGGGGAGTCTATCCGTACTGCACA
GGAAGAAACGCGAGGGTAAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase 1; LysRS 1

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATEDIVGVAGRVIFARNSGKLCFA
TLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVHGAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESR
VRQRYVDLIVRPEARAVARLRIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKR
CIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWA
TIQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAIHDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPL
TRQHRSIPGVTEKWDLYLRGIELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGID
RLLMSLTGLSIRETVLFPIVRPHSN

Sequences:

>Translated_505_residues
MSAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATEDIVGVAGRVIFARNSGKLCFA
TLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVHGAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESR
VRQRYVDLIVRPEARAVARLRIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKR
CIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWA
TIQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAIHDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPL
TRQHRSIPGVTEKWDLYLRGIELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGID
RLLMSLTGLSIRETVLFPIVRPHSN
>Mature_504_residues
SAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATEDIVGVAGRVIFARNSGKLCFAT
LQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVHGAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESRV
RQRYVDLIVRPEARAVARLRIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKRC
IVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWAT
IQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAIHDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPLT
RQHRSIPGVTEKWDLYLRGIELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDR
LLMSLTGLSIRETVLFPIVRPHSN

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=37.4015748031496, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=37.4015748031496, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI45439306, Length=482, Percent_Identity=24.2738589211618, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1789256, Length=507, Percent_Identity=39.0532544378698, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1790571, Length=507, Percent_Identity=38.8560157790927, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87082379, Length=336, Percent_Identity=32.4404761904762, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=28.0632411067194, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=501, Percent_Identity=37.3253493013972, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17535927, Length=490, Percent_Identity=37.7551020408163, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=490, Percent_Identity=37.7551020408163, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI32566633, Length=520, Percent_Identity=22.1153846153846, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=37.1868978805395, Blast_Score=300, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6324256, Length=461, Percent_Identity=29.7180043383948, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24640849, Length=518, Percent_Identity=37.0656370656371, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24640851, Length=518, Percent_Identity=37.0656370656371, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK1_MYCBO (P67608)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857268.1
- ProteinModelPortal:   P67608
- SMR:   P67608
- EnsemblBacteria:   EBMYCT00000017163
- GeneID:   1093140
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3629c
- GeneTree:   EBGT00050000017004
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   MBOV233413:MB3629C-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 55710; Mature: 55579

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATED
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHH
IVGVAGRVIFARNSGKLCFATLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVH
HHHHCCEEEEEECCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEE
GAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESRVRQRYVDLIVRPEARAVARL
CHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
RIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKR
HHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
CIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAI
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GTRQLPLPDGSVYDIDGEWATIQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAI
CCCCCCCCCCCEEECCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPLTRQHRSIPGVTEKWDLYLRG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
IELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGID
EHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHH
RLLMSLTGLSIRETVLFPIVRPHSN
HHHHHHCCCCHHHHHEEEEECCCCC
>Mature Secondary Structure 
SAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATED
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHH
IVGVAGRVIFARNSGKLCFATLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVH
HHHHCCEEEEEECCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEE
GAVISSRRGELSVLADCWRIAAKSLRPLPVAHKEMSEESRVRQRYVDLIVRPEARAVARL
CHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
RIAVVRAIRTALQRRGFLEVETPVLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKR
HHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
CIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTRELIQEVADEAI
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GTRQLPLPDGSVYDIDGEWATIQMYPSLSVALGEEITPQTTVDRLRGIADSLGLEKDPAI
CCCCCCCCCCCEEECCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
HDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPLTRQHRSIPGVTEKWDLYLRG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
IELATGYSELSDPVVQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGID
EHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHH
RLLMSLTGLSIRETVLFPIVRPHSN
HHHHHHCCCCHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972