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The map label for this gene is truA [H]

Identifier: 148663320

GI number: 148663320

Start: 3884729

End: 3885499

Strand: Reverse

Name: truA [H]

Synonym: MRA_3496

Alternate gene names: 148663320

Gene position: 3885499-3884729 (Counterclockwise)

Preceding gene: 148663321

Following gene: 148663315

Centisome position: 87.91

GC content: 68.09

Gene sequence:

>771_bases
GTGGGGCAGCGCACGGTCGCGGGCGATCTCGATGCGGCGCTGACTACCATCTTCCGCACCCCGGTGCGGCTGCGGGCGGC
CGGACGCACTGATGCGGGCGTGCACGCCAGCGGCCAGGTCGCCCACGTTGACGTGCCCGCCGATGCCTTGCCGAACGCTT
ACCCGCGCGCCGGCCACGTCGGTGATCCGGAATTCCTGCCGCTGTTGCGGAGGCTGGGTCGATTTCTGCCCGCCGACGTT
CGAATCCTCGATATCACGCGCGCACCAGCGGGTTTCGACGCCAGATTCTCGGCGCTGCGGCGGCATTACGTGTACCGGCT
GTCGACAGCACCCTACGGTGTCGAGCCGCAGCAGGCGCGCTACATCACCGCTTGGCCACGCGAGCTTGATCTGGATGCGA
TGACGGCCGCGTCGCGAGATTTGATGGGGCTGCACGACTTCGCGGCGTTCTGCCGTCATCGCGAGGGCGCCACCACGATT
CGTGATCTGCAGCGGCTGGACTGGTCGCGTGCCGGCACCCTGGTCACCGCGCACGTCACCGCCGACGCGTTCTGTTGGTC
GATGGTGCGGTCGTTGGTGGGGGCGCTACTGGCGGTCGGCGAGCACCGTCGCGCGACAACTTGGTGTCGTGAGCTGCTCA
CCGCGACAGGACGATCCAGCGACTTCGCGGTCGCGCCGGCGCACGGGTTGACGTTGATCCAGGTGGACTATCCGCCCGAT
GACCAGCTCGCGTCACGGAACTTGGTCACCCGGGATGTGCGCTCGGGCTAA

Upstream 100 bases:

>100_bases
GAAGAGCCCCCCGCAGAGGCCCCCGAGAATTAGCGGCGTTGTCCGTCTTCGGCTCGATATCGCCTACGACGGAACCGATT
TCGCGGGCTGGGCTGCACAA

Downstream 100 bases:

>100_bases
CCCAGGAAGCACATGCTCGGCCTGCTTGTCCGTGGACAAGCACAGCGAACAGACATGCGCACCATGGGTCTGGCAGGCCA
GCATGTCGGGCCGCTCGAAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADV
RILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTI
RDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAVGEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPD
DQLASRNLVTRDVRSG

Sequences:

>Translated_256_residues
MGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADV
RILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTI
RDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAVGEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPD
DQLASRNLVTRDVRSG
>Mature_255_residues
GQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADVR
ILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIR
DLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAVGEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDD
QLASRNLVTRDVRSG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=244, Percent_Identity=33.6065573770492, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321112, Length=209, Percent_Identity=27.7511961722488, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28092; Mature: 27961

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHV
CCCCCCCCHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCC
GDPEFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQAR
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCE
YITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHCCCEEEEEHH
ADAFCWSMVRSLVGALLAVGEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCC
DQLASRNLVTRDVRSG
HHHHHCCHHHHHCCCC
>Mature Secondary Structure 
GQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHV
CCCCCCCHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCC
GDPEFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQAR
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCE
YITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHCCCEEEEEHH
ADAFCWSMVRSLVGALLAVGEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCC
DQLASRNLVTRDVRSG
HHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]