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The map label for this gene is 148663096

Identifier: 148663096

GI number: 148663096

Start: 3620564

End: 3621154

Strand: Reverse

Name: 148663096

Synonym: MRA_3274

Alternate gene names: NA

Gene position: 3621154-3620564 (Counterclockwise)

Preceding gene: 148663097

Following gene: 148663095

Centisome position: 81.93

GC content: 62.44

Gene sequence:

>591_bases
GTGATTGCCGGTGCCTTGGGCAACTGGCTGATGTCACGCGGTGAAGCTGTGGCACCGACCGCGACTGTTCGGGCGATGGC
GCCGCTGTCGGTCTATGCCGACGACCAACTCGACTCAACCGGCCCTGGTCAGGCGATCAGCCAGGTGACACCGTTCTTGG
TTGACCTCCCGGTGGGGGAGGGCAATGCCGTAGTGCGGTTGTCGCAGATCGCGCACGCCACCGAATCCAACCCGACCGCC
GCCAGCCTGGTCGATGCCAGGACTATCGTCACGTTGTCGGGGTTAGCACCGGCCACTTTGCACGCCATGGGGGTGCGGGT
CGCCACTAGCTTTTCGGCACGGTTGTTCAATCTGTTGATCACCAATGCCCCCGGAACCCAGTCGCAGATGTACATTGCGG
GTACCAAGCTGCTGGAGACCTACTCCGTGCCGCCGCTACTGCACAACCAGGCGCTGGCCATCAGCGTGACGTCGTATAAC
GGGATGTTGTATTTCGGCATCAATGCCGACCGCGACGCGATGAGCGATGTCGATCTGCTGCCGGGGCTGTTGAGCCAAGC
ACTGGACGAGCTTCTGGAGGCTTCGCGGTAG

Upstream 100 bases:

>100_bases
TGTCGCGTAATCGGCGATTTACGGTTGCCCGTGCAAGTCTTGATGACTATCGCACGGTGCGGGCTCGCTACGACTGTGAC
TCCACGACGTGGTGCTGACG

Downstream 100 bases:

>100_bases
TTGTTAGGGGTCGATGGTGATGTGTGGATATACCATCCGTTGATGTGAGCACCGCGACCAACGACGGCGCGTCGTCGAGA
GCGAAGGGACACAGATCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1760/MT1809 [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MIAGALGNWLMSRGEAVAPTATVRAMAPLSVYADDQLDSTGPGQAISQVTPFLVDLPVGEGNAVVRLSQIAHATESNPTA
ASLVDARTIVTLSGLAPATLHAMGVRVATSFSARLFNLLITNAPGTQSQMYIAGTKLLETYSVPPLLHNQALAISVTSYN
GMLYFGINADRDAMSDVDLLPGLLSQALDELLEASR

Sequences:

>Translated_196_residues
MIAGALGNWLMSRGEAVAPTATVRAMAPLSVYADDQLDSTGPGQAISQVTPFLVDLPVGEGNAVVRLSQIAHATESNPTA
ASLVDARTIVTLSGLAPATLHAMGVRVATSFSARLFNLLITNAPGTQSQMYIAGTKLLETYSVPPLLHNQALAISVTSYN
GMLYFGINADRDAMSDVDLLPGLLSQALDELLEASR
>Mature_196_residues
MIAGALGNWLMSRGEAVAPTATVRAMAPLSVYADDQLDSTGPGQAISQVTPFLVDLPVGEGNAVVRLSQIAHATESNPTA
ASLVDARTIVTLSGLAPATLHAMGVRVATSFSARLFNLLITNAPGTQSQMYIAGTKLLETYSVPPLLHNQALAISVTSYN
GMLYFGINADRDAMSDVDLLPGLLSQALDELLEASR

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 20482; Mature: 20482

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGALGNWLMSRGEAVAPTATVRAMAPLSVYADDQLDSTGPGQAISQVTPFLVDLPVGE
CCCCHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCC
GNAVVRLSQIAHATESNPTAASLVDARTIVTLSGLAPATLHAMGVRVATSFSARLFNLLI
CCEEEEEHHHHHHCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEE
TNAPGTQSQMYIAGTKLLETYSVPPLLHNQALAISVTSYNGMLYFGINADRDAMSDVDLL
ECCCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHH
PGLLSQALDELLEASR
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAGALGNWLMSRGEAVAPTATVRAMAPLSVYADDQLDSTGPGQAISQVTPFLVDLPVGE
CCCCHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCC
GNAVVRLSQIAHATESNPTAASLVDARTIVTLSGLAPATLHAMGVRVATSFSARLFNLLI
CCEEEEEHHHHHHCCCCCCHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEE
TNAPGTQSQMYIAGTKLLETYSVPPLLHNQALAISVTSYNGMLYFGINADRDAMSDVDLL
ECCCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHH
PGLLSQALDELLEASR
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]