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The map label for this gene is tgs3

Identifier: 148663097

GI number: 148663097

Start: 3621157

End: 3621972

Strand: Reverse

Name: tgs3

Synonym: MRA_3275

Alternate gene names: 148663097

Gene position: 3621972-3621157 (Counterclockwise)

Preceding gene: 148663099

Following gene: 148663096

Centisome position: 81.95

GC content: 64.34

Gene sequence:

>816_bases
GTGGTCACCCGGTTGTCTGCATCGGACGCCTCCTTCTATCAGCTGGAGAACACAGCCACCCCAATGTATGTCGGGTTGCT
GTTGATCCTGCGCCGTCCGCGTGCCGGATTGAGCTACGAGGCACTGCTGGAAACCGTCGAACAGCGGCTGCCGCAGATAC
CGCGCTATCGTCAGAAAGTCCAGGAAGTGAAATTGGGCCTGGCCAGGCCGGTGTGGATCGACGATCGCGACTTCGACATC
ACCTACCACGTCCGGCGGTCGGCCCTGCCGTCGCCGGGTAGTGACGAGCAACTGCACGAGCTGATCGCACGGCTGGCCGC
TCGGCCGCTGGACAAATCGCGGCCATTGTGGGAGATGTATCTCGTCGAGGGTCTGGAAAAGAATCGCATTGCCCTCTACA
CCAAGTCGCACCAAGCCCTGATCAACGGGGTGACCGCGCTGGCGATAGGCCACGTGATCGCCGACCGGACGCGGCGTCCA
CCGGCGTTTCCGGAAGACATCTGGGTCCCGGAACGGGACCCCGGCACCACGCGGTTGCTGCTGCGCGCGGTCGGGGACTG
GCTGGTGCGGCCGGGCGCACAACTACAGGCCGTCGGATCCGCGGTCGCCGGGTTGGTAACGAATTCCGGCCAACTCGTCG
AGACGGGCCGCAAGGTTCTCGATATCGCCCGCACGGTGGCACGGGGCACCGCGCCCAGTAGCCCGCTCAATGCCACCGTG
TCGCGTAATCGGCGATTTACGGTTGCCCGTGCAAGTCTTGATGACTATCGCACGGTGCGGGCTCGCTACGACTGTGACTC
CACGACGTGGTGCTGA

Upstream 100 bases:

>100_bases
GGGCTTGCGTGTACCCGCGCCGTGAAGTGGTCGGGCTAACCGTTAGCGTGGGCGACGACGTGACTCGTCGCGACGAATTG
ACAAGGAGGACAGGGCCGTA

Downstream 100 bases:

>100_bases
CGGTGATTGCCGGTGCCTTGGGCAACTGGCTGATGTCACGCGGTGAAGCTGTGGCACCGACCGCGACTGTTCGGGCGATG
GCGCCGCTGTCGGTCTATGC

Product: hypothetical protein

Products: NA

Alternate protein names: TGS3; Probable triacylglycerol synthase tgs3

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVARASLDDYRTVRARYDCDSTTWC

Sequences:

>Translated_271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVARASLDDYRTVRARYDCDSTTWC
>Mature_271_residues
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDI
TYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRP
PAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
SRNRRFTVARASLDDYRTVRARYDCDSTTWC

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGS3_MYCTU (O05879)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   D70591
- RefSeq:   NP_217751.1
- RefSeq:   NP_337860.1
- ProteinModelPortal:   O05879
- EnsemblBacteria:   EBMYCT00000003074
- EnsemblBacteria:   EBMYCT00000072985
- GeneID:   888767
- GeneID:   922712
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3331
- KEGG:   mtu:Rv3234c
- TIGR:   MT3331
- TubercuList:   Rv3234c
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   HASLDDY
- ProtClustDB:   CLSK792366
- InterPro:   IPR004255

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 30379; Mature: 30379

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMY
HHHHHCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLL
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SRNRRFTVARASLDDYRTVRARYDCDSTTWC
CCCCEEEEEECCHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
QEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMY
HHHHHCCCCCEEECCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLL
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SRNRRFTVARASLDDYRTVRARYDCDSTTWC
CCCCEEEEEECCHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036