Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 148662723

GI number: 148662723

Start: 3201575

End: 3202162

Strand: Reverse

Name: rlmN [H]

Synonym: MRA_2904

Alternate gene names: 148662723

Gene position: 3202162-3201575 (Counterclockwise)

Preceding gene: 148662724

Following gene: 148662722

Centisome position: 72.45

GC content: 65.82

Gene sequence:

>588_bases
ATGTGGTGTTCATGGGTATGGGGGGAGCCGCTGGCCAACTACGCCAGGGTGTTGGCCGCAGTTCAGCGCATTACCGCGCG
GCCGCCGTCCGGTTTCGGGATTTCGGCCCGCGCGGTGACGGTGTCGACGGTGGGTCTGGCCCCTGCTATCCGCAACCTTG
CCGACGCGCGGCTCGGCGTGACCCTGGCGCTGTCGCTGCACGCCCCCGACGACGGGTTGCGCGATACACTAGTTCCGGTC
AACAACCGGTGGAGGATCAGCGAAGCGCTCGATGCGGCCCGGTACTACGCCAATGTGACCGGGCGACGGGTGTCTATTGA
GTACGCGCTGATCCGTGATGTCAACGACCAACCGTGGCGGGCCGACTTGCTGGGAAAGCGACTGCATCGTGTTCTCGGGC
CGTTGGCGCACGTGAACCTGATCCCGCTGAACCCGACTCCGGGCAGTGATTGGGATGCCAGCCCCAAGCCGGTTGAGCGT
GAATTCGTCAAACGGGTTCGGGCGAAAGGAGTTTCCTGCACGGTACGAGACACCCGTGGGCGGGAGATCAGCGCCGCCTG
CGGACAGCTGGCCGCCGTAGGCGGGTAG

Upstream 100 bases:

>100_bases
CAGGGCGGATTGACTCGCAACCTATCGACGGCGGAGATCCTCGAGCAGGTGCGTGCCGGCGCCGCGGCGTTGCGCGACGA
CTTCGGCGATCGGTTGTCGA

Downstream 100 bases:

>100_bases
CTAGAGCGCAGGCGAATCAGCCAGCGCTGCGGACAGCTGGCCGCCGTAGGCGGGTAGCTGGAGCGCAGGCGAATCAGCCA
GCGCTGCGGACAGCTGGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MWCSWVWGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDDGLRDTLVPV
NNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDWDASPKPVER
EFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG

Sequences:

>Translated_195_residues
MWCSWVWGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDDGLRDTLVPV
NNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDWDASPKPVER
EFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG
>Mature_195_residues
MWCSWVWGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDDGLRDTLVPV
NNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDWDASPKPVER
EFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=189, Percent_Identity=41.2698412698413, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 21207; Mature: 21207

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWCSWVWGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCE
TLALSLHAPDDGLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWR
EEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCH
ADLLGKRLHRVLGPLAHVNLIPLNPTPGSDWDASPKPVEREFVKRVRAKGVSCTVRDTRG
HHHHHHHHHHHHCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCC
REISAACGQLAAVGG
CCHHHHHHHHHHCCC
>Mature Secondary Structure
MWCSWVWGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCE
TLALSLHAPDDGLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWR
EEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCH
ADLLGKRLHRVLGPLAHVNLIPLNPTPGSDWDASPKPVEREFVKRVRAKGVSCTVRDTRG
HHHHHHHHHHHHCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCC
REISAACGQLAAVGG
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]