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The map label for this gene is rlmN [H]

Identifier: 148662724

GI number: 148662724

Start: 3201843

End: 3202670

Strand: Reverse

Name: rlmN [H]

Synonym: MRA_2905

Alternate gene names: 148662724

Gene position: 3202670-3201843 (Counterclockwise)

Preceding gene: 148662725

Following gene: 148662723

Centisome position: 72.46

GC content: 67.39

Gene sequence:

>828_bases
ATGGTCCCCGAGTTGATGTTCGATGAGCCGCGTCCGGGTCGGCCGCCGCGGCACCTGGCCGATCTCGACGCGGCGGGCCG
AGCGTCTGCCGTCGCGGAGCTGGGGCTGCCGGCGTTTCGGGCCAAGCAGCTTGCACACCAGTACTACGGTCGGCTGATCG
CCGATCCGCGTCAGATGACCGACCTTCCGGCGGCCGTTCGCGACCGGATCGCCGGAGCGATGTTCCCGAACTTGCTTACC
GCGTCCGCCGACATCACCTGCGATGCCGGCCAGACTCGAAAGACGTTGTGGCGGGCCGTCGACGGTACCATGTTCGAATC
GGTGCTGATGCGCTATCCGCGGCGCAACACGGTCTGCATTTCCTCGCAGGCCGGCTGCGGCATGGCTTGCCCGTTCTGTG
CCACTGGCCAGGGCGGATTGACTCGCAACCTATCGACGGCGGAGATCCTCGAGCAGGTGCGTGCCGGCGCCGCGGCGTTG
CGCGACGACTTCGGCGATCGGTTGTCGAATGTGGTGTTCATGGGTATGGGGGGAGCCGCTGGCCAACTACGCCAGGGTGT
TGGCCGCAGTTCAGCGCATTACCGCGCGGCCGCCGTCCGGTTTCGGGATTTCGGCCCGCGCGGTGACGGTGTCGACGGTG
GGTCTGGCCCCTGCTATCCGCAACCTTGCCGACGCGCGGCTCGGCGTGACCCTGGCGCTGTCGCTGCACGCCCCCGACGA
CGGGTTGCGCGATACACTAGTTCCGGTCAACAACCGGTGGAGGATCAGCGAAGCGCTCGATGCGGCCCGGTACTACGCCA
ATGTGACCGGGCGACGGGTGTCTATTGA

Upstream 100 bases:

>100_bases
GGCGGTCTGATGGACCGGCTCGACGGCATACTGCCTTCCGCGGTGGCGGCCTGGATAGTCCTCACACTGCTGCCCTGACA
ACGGCGATACTGGACAGGTC

Downstream 100 bases:

>100_bases
GTACGCGCTGATCCGTGATGTCAACGACCAACCGTGGCGGGCCGACTTGCTGGGAAAGCGACTGCATCGTGTTCTCGGGC
CGTTGGCGCACGTGAACCTG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGGAAGQLRQGVGRSSAHYRAAAVRFRDFGPRGDGVDGGSGPCYPQPCRRAARRDPGAVAARPRR
RVARYTSSGQQPVEDQRSARCGPVLRQCDRATGVY

Sequences:

>Translated_275_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGGAAGQLRQGVGRSSAHYRAAAVRFRDFGPRGDGVDGGSGPCYPQPCRRAARRDPGAVAARPRR
RVARYTSSGQQPVEDQRSARCGPVLRQCDRATGVY
>Mature_275_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGGAAGQLRQGVGRSSAHYRAAAVRFRDFGPRGDGVDGGSGPCYPQPCRRAARRDPGAVAARPRR
RVARYTSSGQQPVEDQRSARCGPVLRQCDRATGVY

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 29599; Mature: 29599

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAAVRDRIAGAMFPNLLTASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCI
HCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGGAA
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCH
GQLRQGVGRSSAHYRAAAVRFRDFGPRGDGVDGGSGPCYPQPCRRAARRDPGAVAARPRR
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHH
RVARYTSSGQQPVEDQRSARCGPVLRQCDRATGVY
HHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAAVRDRIAGAMFPNLLTASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCI
HCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGGAA
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCH
GQLRQGVGRSSAHYRAAAVRFRDFGPRGDGVDGGSGPCYPQPCRRAARRDPGAVAARPRR
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHH
RVARYTSSGQQPVEDQRSARCGPVLRQCDRATGVY
HHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]