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The map label for this gene is plcA

Identifier: 148662179

GI number: 148662179

Start: 2640441

End: 2641979

Strand: Reverse

Name: plcA

Synonym: MRA_2371

Alternate gene names: 148662179

Gene position: 2641979-2640441 (Counterclockwise)

Preceding gene: 148662181

Following gene: 148662178

Centisome position: 59.77

GC content: 62.9

Gene sequence:

>1539_bases
ATGTCACGTCGAGAGTTTTTGACAAAGCTCACTGGCGCAGGCGCAGCGGCATTCCTGATGGACTGGGCTGCACCGGTGAT
TGAAAAGGCCTACGGCGCCGGGCCTTGTCCCGGACATTTGACCGACATCGAGCATATCGTGTTGCTGATGCAGGAGAACC
GGTCATTCGACCACTATTTCGGAACGCTTTCCAGCACCAATGGGTTCAACGCCGCGTCGCCGGCATTCCAACAAATGGGT
TGGAACCCCATGACGCAGGCGTTGGACCCCGCCGGGGTCACCATTCCGTTCCGCTTGGACACCACCCGAGGCCCCTTCCT
GGACGGCGAGTGCGTCAACGACCCCGAGCACCAGTGGGTGGGGATGCACCTGGCCTGGAACGGTGGTGCCAACGACAACT
GGCTGCCGGCGCAGGCGACCACCCGCGCAGGACCATATGTCCCTTTGACCATGGGTTACTACACGCGCCAAGACATCCCG
ATCCACTATCTGCTGGCCGACACGTTCACCATCTGCGACGGCTACCATTGCTCGCTGCTGACGGGCACCCTGCCCAACCG
GCTCTACTGGTTGAGCGCCAACATCGACCCCGCCGGCACCGACGGGGGACCCCAATTGGTAGAGCCGGGCTTCCTGCCGC
TGCAGCAATTCAGTTGGCGCATCATGCCGGAAAACCTCGAAGATGCCGGGGTCAGCTGGAAGGTGTACCAGAACAAGGGC
CTCGGGCGATTCATCAACACGCCCATCAGCAATAACGGGCTGGTGCAGGCCTTCCGCCAGGCAGCTGATCCGAGGTCGAA
CTTGGCCCGCTACGGTATCGCCCCGACCTACCCTGGGGACTTCGCTGCCGACGTCAGGGCCAACCGGCTACCCAAGGTCT
CCTGGTTAGTTCCCAACATCCTGCAGTCCGAACACCCCGCCCTGCCGGTAGCGCTTGGCGCGGTGTCCATGGTGACCGCG
CTGCGGATCTTGCTGTCCAATCCCGCGGTGTGGGAAAAGACCGCACTTATCGTCAGCTATGACGAGAACGGCGGCTTCTT
CGACCACGTCACGCCCCCCACGGCACCGCCCGGGACACCCGGCGAATTCGTCACGGTGCCCAACATCGACGCAGTACCCG
GGTCCGGTGGCATTCGTGGTCCGCTCGGTCTGGGTTTTCGCGTTCCCTGCATTGTCATTTCGCCGTACAGCCGCGGCCCG
CTGATGGTCTCCGACACGTTCGACCACACCTCGCAATTGAAGTTGATTCGCGCCCGGTTCGGCGTGCCGGTTCCCAACAT
GACCGCCTGGCGCGACGGCGTGGTTGGCGACATGACCTCAGCGTTCAACTTTGCGACTCCACCGAATTCGACCAGACCCA
ACTTGAGCCACCCGTTGCTGGGAGCGCTGCCGAAGCTGCCGCAGTGCATCCCTAACGTGGTGTTGGGAACCACCGACGGC
GCGTTGCCGAGCATTCCCTATCGGGTGCCCTATCCGCAGGTGATGCCAACTCAGGAAACCACACCCGTCCGCGGGACTCC
CAGCGGGCTGTGCAGCTGA

Upstream 100 bases:

>100_bases
CGCCGGTTGGCGTATCGCGATGGACCGCTGTCGTCACTATAACGTGATCTGAGCTGAGTGCAGGAAGGCAGGGCAAGTGA
GCGCAAGCCCACTCCTCGGA

Downstream 100 bases:

>100_bases
AATTTCCCGGTAATCTCCCGGCGTTCGATTTGCTTGCATGTGCGCGAGCAAATCGCCCGCACCAATCCCGCGCCCGCCCC
GGCAGGCAGGCGGAATCAGA

Product: membrane-associated phospholipase C 1

Products: NA

Alternate protein names: MTP40 antigen

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAASPAFQQMG
WNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIP
IHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKG
LGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVALGAVSMVTA
LRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGP
LMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDG
ALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS

Sequences:

>Translated_512_residues
MSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAASPAFQQMG
WNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIP
IHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKG
LGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVALGAVSMVTA
LRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGP
LMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDG
ALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
>Mature_511_residues
SRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAASPAFQQMGW
NPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPI
HYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKGL
GRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVALGAVSMVTAL
RILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPL
MVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDGA
LPSIPYRVPYPQVMPTQETTPVRGTPSGLCS

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHLA_MYCTU (Q04001)

Other databases:

- EMBL:   L11868
- EMBL:   L11868
- EMBL:   U49511
- EMBL:   BX842579
- EMBL:   AE000516
- EMBL:   M57952
- EMBL:   S69737
- PIR:   H70662
- RefSeq:   NP_216867.1
- RefSeq:   NP_336895.1
- EnsemblBacteria:   EBMYCT00000002239
- EnsemblBacteria:   EBMYCT00000070413
- GeneID:   885995
- GeneID:   925941
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2416
- KEGG:   mtu:Rv2351c
- TIGR:   MT2416
- TubercuList:   Rv2351c
- GeneTree:   EBGT00050000017446
- HOGENOM:   HBG565180
- OMA:   FINTPIS
- ProtClustDB:   CLSK791798
- BRENDA:   3.1.4.3
- GO:   GO:0009405
- InterPro:   IPR007312
- InterPro:   IPR006311
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04185 Phosphoesterase

EC number: =3.1.4.3

Molecular weight: Translated: 55382; Mature: 55250

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYF
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEECCCCCHHHC
GTLSSTNGFNAASPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWV
CCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
GMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPIHYLLADTFTICDGYHCSLL
EEEEEECCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCEEEEEECHHHHCCCCEEEEE
TGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKG
ECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCEEEEECCCC
LGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNI
CCCEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCEEHHHHHHH
LQSEHPALPVALGAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTP
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCCC
GEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPLMVSDTFDHTSQLKLIRARF
CCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
GVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDG
CCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCC
ALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYF
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHEEEEEECCCCCHHHC
GTLSSTNGFNAASPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWV
CCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
GMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPIHYLLADTFTICDGYHCSLL
EEEEEECCCCCCCEECCCCCCCCCCCEEEEECCEECCCCCEEEEEECHHHHCCCCEEEEE
TGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKG
ECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCEEEEECCCC
LGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNI
CCCEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCEEHHHHHHH
LQSEHPALPVALGAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTP
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCCC
GEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPLMVSDTFDHTSQLKLIRARF
CCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
GVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDG
CCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCC
ALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7591062; 8757862; 9634230; 12218036; 1909999; 8298518; 9114405